Spatial Evolution of Tourism-Oriented Rural Residential Space: A Case Study of Xiaochuanfangyu Village,Dongshuichang Village and Maojiayu Village in Jizhou District,Tianjin

Currently, rural residential space can't continue to meet the growing demand for tourism receptions because of the development of rural tourism, resulting in the evolution and reconstruction of residential space in rural areas. In the past decade, rural tourism has become the main driving force for industrial transformation and economic growth in rural areas of China. Taking three tourism-oriented villages in Jizhou District, Tianjin, namely Xiaochuanfangyu Village, Dongshuichang Village and Maojiayu Village, as the objects of the research, this paper analyzed the influence of rural tourism on the evolution of rural residential space from the perspectives of the spatial pattern and architectural style at the microscopic scale.     

微波杀青对茶在制品物理特性影响的初探

以一芽一叶的福鼎大白茶鲜叶为原料,设定6个微波杀青的时间梯度(0、30、60、90、120、150 s),按时间点取样,测定在制品的含水率、容重、色差Lab值、长度、宽度等,研究微波杀青时间对茶在制品物理特性的影响。结果表明,随着微波杀青的进行,在制品的含水率在前90 s匀速缓慢下降,而后急速下降,容重在微波30 s后显著下降;茶在制品的平均相对长度差、相对宽度差和面积差呈现先降后升的趋势,在微波60~90 s时相对最小;在制品色差L值、|a|值、b值随着微波杀青的进行,呈先升后降的趋势,微波前90 s均较鲜叶有显著提升,而后显著下降。相关性分析表明,微波杀青时间和含水率均对L值、|a|值、b值、相对长度差等呈显著影响,总体上看以微波杀青60~90 s最有利于获得较优的外形属性。     

大豆对大豆胞囊线虫侵染的应答机制研究

大豆胞囊线虫病(Heterodera glycines,soybean cyst nematode,SCN)是大豆生产上的重要病害,其特点为危害重、分布广、难防治,每年对大豆生产造成极大的损失。种植大豆抗性新品种是防治SCN目前最为有效的措施,研究大豆对SCN侵染的应答机制,是培育大豆持久抗病品种的前提,对加快抗线虫品种选育及SCN的防控具有重要的意义。本文综述了大豆对SCN侵染的组织细胞学应答机制;介绍了大豆在SCN侵染后酶系变化及酚类代谢的生理生化应答机制;从分子水平阐明了SCN侵染后大豆的基因转录变化,差异蛋白及DNA甲基化的应答机制,以期为大豆胞囊线虫病害的进一步研究与防治提供参考。     

Protective effect of curcumin analogue L6H4 on diaphragm of type 2 diabetic rats

AIM: To investigate the protective effect of curcumin analogue L6H4 on diaphragm of type 2 diabetic rats.METHODS: SPF male Sprague-Dawley rats (n=40) were randomly divided into 5 groups: normal control (NC) group, high fat (HF) group, high fat+L6H4 treatment (FT) group, diabetes mellitus (DM) group and DM+L6H4 treatment (DT) group. The rats in the later 4 groups were fed with high-fat diet. After 4 weeks of high-fat diet fee-ding, the rats in DM and DT groups were intraperitoneally injected with streptozotocin to induce type 2 diabetes melliutus. The rats in FT and DT groups were given L6H4 by gavage for 8 weeks. Blood glucose and blood lipid levels were detected biochemically. Fasting serum insulin (FINS) level was measured by radioimmunoassay and insulin resistance index (HOMA-IR) was calculated. Serum adiponectin (APN) level was measured by ELISA. The morphological changes of the diaphragm were observed under light and transmission electron microscopes. Lipid deposition and the activity of succinate dehydrogenase (SDH) and NADH-tetrazolium reductase (NADH-TR) were observed by enzyme histochemical staining. The content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in the diaphragm were measured by thiobarbituric acid method and hydroxylamine method, respectively. The protein expression of adiponectin receptor 1 (AdipoR1) in the diaphragm was determined by immunohistochemistry and Western blot. RESULTS: The levels of blood lipids, blood glucose, FINS and HOMA-IR in HF and DM groups were higher than those in NC group, but decreased after L6H4 treatment. The serum APN level in HF and DM groups was lower than that in NC group, but increased after treatment with L6H4. The muscle fibers of the diaphragm were shrunk, fat particles accumulated in the muscle fibers, and the mitochondria were slightly swollen in HF and DM groups. The diaphragmatic fibrosis was obvious in DM group. These lesions were relieved after L6H4 treatment. Compared with NC group, the level of MDA and the activity of SDH and NADH-TR in the diaphragm were increased in HF and DM groups, but decreased after treatment with L6H4. The activity of SOD and the expression of AdipoR1 in the diaphragm were lower than those in NC group, but increased after L6H4 treatment.CONCLUSION: The curcumin analogue L6H4 exerts a protective effect on diaphragm in type 2 diabetic rats. The strengthened protein expression of AdipoR1, the increased serum level of APN, and anti-lipid peroxidation may be involved in the process.     

辣椒GRAS家族全基因组鉴定与表达分析

基于辣椒全基因组数据信息,利用生物信息学方法对CaGRAS基因家族进行了系统鉴定和进化分析,并通过Real-time PCR方法检测了CaGRAS家族组织表达模式和对PEG6000、盐胁迫的应答。结果表明:在辣椒中存在54个CaGRAS基因,除11号染色体外其余染色体均有分布,外显子数1~3,等电点4.97~9.13;系统进化分析显示CaGRAS基因可分为8个进化群;CaGRAS基因具有不同的表达模式,在根、茎、叶片和茎尖中优势表达的基因分别有34、8、3和8个;多数CaGRAS基因能响应PEG6000和盐胁迫,其中CaGRAS11、CaGRAS30、CaGRAS40、CaGRAS44和CaGRAS50受到PEG6000和盐胁迫的强烈诱导。本研究为深入解析CaGRAS家族基因的功能奠定了一定基础。     

海拔高度对茅莓果实品质的影响

对梵净山同一山体、同一坡面上800~2 000m不同海拔高度2年生茅莓果实品质进行了分析研究,以期为高海拔地区茅莓栽培选择适宜的生态区提供参考依据。结果表明:可滴定酸含量与海拔高度呈负相关;单果质量、可溶性固形物含量、固酸比、维生素C含量、可溶性蛋白质含量在海拔800~1 800 m区段,随着海拔的升高逐渐增加;而在1 800~2 000m区段,呈下降趋势;在海拔1 600~1 800m区段茅莓的单果质量、可溶性固形物含量、固酸比、维生素C含量、可溶性蛋白质含量与其它海拔区段差异显著(P0.05),表现出很好的品质特性,是适合茅莓优质高产栽培的海拔地带。     

供钾水平对小白菜同化物及钾素分配的影响

以"苏州青"和"矮脚黄"2个小白菜品种为试材,采用室内模拟砂培培养法,研究了供钾水平(0.0、2.5、5.0、7.5、10.0mmol·L~(-1))对其根、叶柄和叶片同化物及钾素分配的影响,以期为采用钾高效利用技术提高小白菜产投比的钾营养诊断相关研究奠定基础。结果表明:供钾水平对小白菜生物量的影响随其生育期的延长而增大。其中,"苏州青"的同化物分配对供钾水平的响应较"矮脚黄"相对敏感,其可食部分生物量(地上部生物量、产量)最大时的供钾水平相对低于"矮脚黄",而植株不同部位钾含量最高时的供钾水平却相对高于"矮脚黄"。小白菜植株不同部位钾含量的高低顺序依次为叶柄、叶片和根系,且不同部位钾含量最高时所需要的外源供钾水平及钾在小白菜植株不同部位的分配百分比均表现为叶片叶柄根系,说明小白菜植株不同部位对供钾水平的敏感性存在明显差异。适宜的供钾水平可能通过协调同化物在植株不同部位的分配,并维持分配比例的稳定性,达到促进小白菜生长的效果。与正常供钾水平比,供试的2个品种小白菜对过量钾的响应也存在一定的差异:分别通过明显降低"苏州青"的根系钾含量及矮脚黄的根系同化物的分配,而减少了钾在其各自根系中的分配。缺钾通过降低同化物,特别是钾在小白菜叶柄中的分配而明显增加了其向叶片和根系的分配,进而提高了小白菜的根冠比。随着供钾水平的升高,只有叶柄的同化物和钾分配的百分比均有所升高,且以钾分配百分比的升高幅度远大于同化物。综上所述,叶柄钾含量和叶柄钾分配百分比对小白菜钾营养状况存在较好的指示作用。     

广8优673试种表现及高产栽培技术要点

广8优673是用福建省农业科学院水稻研究所选育的福恢673与广东省农业科学院水稻所育成的优质抗病三系不育系广8A配组而成的三系晚籼杂交稻新品种,具有群体整齐、穗大粒多、丰产性好、适应性广、米质较优等特点,2015年通过福建省农作物品种审定。总结其特征特性及高产栽培技术要点。     

禅茶馆的定位与室内设计初探

以禅茶馆——茶馆领域新业态为研究对象,在探讨了茶文化与禅文化相互交融形成"茶禅一味"理念的基础上,对禅茶馆进行了定义,围绕着茶馆"禅意"的呈现,提出了"六根共识"——即"眼、鼻、耳、舌、身、意"的全方位感受的室内设计方案,以期为禅茶馆的设计提供有益参考,传承和弘扬中华优秀传统文化。     

番茄青枯病抑病土壤根际微生物群落特征及其抑制性传递机制

根际微生物在宿主植物抵御土传病害发生过程中发挥着重要作用。为探究抑病土壤与感病土壤根际微生物群落特征及其微生物群落构建机制,分别采集抑病土和感病土中的番茄根际土壤,采用实时荧光定量PCR技术检测两种根际土壤中的病原菌含量,并利用16SrRNA基因扩增子高通量测序技术分析抑病和感病土中番茄根际土壤微生物群落多样性、组成、结构以及基于零模型的微生物群落构建机制的差异。结果表明,与感病土相比,抑病土壤中番茄的青枯病病情指数明显降低（病情指数分别为47.5和22.5）,其根际中细菌群落具有更高的alpha多样性、更丰富的放线菌门、厚壁菌门以及芽孢杆菌科和链霉菌科等有益微生物,较低的青枯病菌丰度（病原菌丰度降低了12.22倍）并伴随着较高的随机性过程,因此抑病土壤受病害胁迫的适应性较强。将感病土壤与抑病土壤按照一定比例进行混合,形成了仅有感病土D10H0、感病土与抑病土质量比为1︰1的D5H5以及仅有抑病土D0H10这三种处理以进一步检测抑病土壤抑病特性的可传递性。结果表明,随着抑病土比例的增加,番茄青枯病病情指数逐步降低,D10H0、D5H5和D0H10的病情指数分别为41.67、29.17与...