Reproductive cycle of the south American scallop Amusium laurenti (Gmelin, 1791) (Bivalvia, Pectinidae)

Amusium laurenti is a functional hermaphroditewith well differentiated male and female gonadalareas. The gonadal index varies throughout the yearwith maximum values occurring in January, March andJuly, and minimum ones in August and September. Partial spawning (with respect to the individual andthe population) occurred from January to February andMarch to May. The major spawning event coincided withthe end of the period during which bottom watertemperature increased to temperatures above 26°C(July–August). Subsequently temperature decreased to22°C, with a period of gonadal restoration andincreasing gonadal index. Histological analysis ofthe gonads correlated with the gonadal index.     

Evaluation of estimated weather data for calculating Penman-Monteith reference crop evapotranspiration

Utilizing the weather generator ClimGen, daily solar radiation (R_s) and vapor pressure deficit (VPD) were estimated from temperature data and used to calculate evapotranspiration at five locations, representing tropical, temperate, semi-arid, and arid climates. ClimGen was calibrated for each location using the most recent 2 or 5 years of complete daily weather records. Actual and estimated values were compared on a daily and weekly (7-day running average) basis. Error indices were defined to indicate excellent to poor performance of the estimation methods. Overall in all locations, the ClimGen estimates for both daily R_s and VPD were poor to acceptable. The weekly analyses showed significant improvement in performance for both R_s and VPD estimations in arid and semi-arid locations. Daily reference crop evapotranspiration values using the FAO Penman-Monteith equation (PM ET_o) were calculated using complete daily weather records. These values were compared with (1) ET_o calculated with the PM model, actual temperature data, and ClimGen estimates of daily R_s, VPD, and generated wind speed (PM_Est ET_o), and (2) ET_o calculated solely from actual daily temperature data using a calibrated version of the Hargreaves method (HG_Adj ET_o). The daily PM_Est ET_o results were poor to acceptable in all locations, but analyses for weekly periods showed improved performance to acceptable and good levels for arid and semi-arid locations. The performance of the HG_Adj ET_o method was also poor to acceptable for daily ET estimates in all locations, while weekly analyses showed improvement. A non-calibrated version of the Hargreaves method did not work for either daily or weekly periods. The PM_Est ET_o and HG_adj ET_o methods appeared suitable for weekly periods in arid and semi-arid locations provided that at least 2 years of complete weather records were available to calibrate the parameters required. There was no advantage in using 5 years of weather records for calibration.Communicated by E. Fereres     

The soils of the eastern hilly area and their suitability to vineyard in Friuli (Italy)

Typic Eutrochrepts (from “Flysch”), Typic Udifluvents (from alluvial deposits), Dystric Eutrochrepts and Plinthic Paleudults on old alluvial deposits and terraces were characterized. All soils investigated fall in the suitability class S2 or S3 for grape cultivation. Imperfect drainage and acidic reaction are most prominent soil limitations. Fertilization with organic matter, N and some microelements such as Zn, as well as drainage improvement, are the suggestions proposed in order to increase the soil fertility and the wine production.     

Stable isotope characterization of the ortho-oxygenated phenylpropanoids: coumarin and melilotol

The natural abundance 2H NMR spectra of extractive coumarin 10 and of its dihydroderivative melilotol 11 produced by baker's yeast reduction has been compared with synthetic materials. Diagnostic for the differentiation of 10 are the (D/H)beta values, which are in the 128.1-133.6 ppm interval for the natural compounds but 258.5 and 189.8 ppm for the synthetic materials. Such a dramatic difference is also found for methyl cinnamate 12, which shows (D/H)beta values of 127.2 and 515.8 ppm, respectively. In extractive 10, the ratio (D/H)4para/(D/H)6ortho = 1.24 is similar to that observed in structurally related salicin and methyl salicylate. Coumarin 10 is transformed in salicyl alcohol 9, providing diacetate 14, showing in the natural series the trend (D/H)3meta > (D/H)4para > (D/H)5meta approximately (D/H)6ortho. A similar trend is shown also by the synthetic 10. A clear distinction between extractive and synthetic 10 is obtained through delta18O determinations on 10 and on chroman 13. The bulk delta18O values in the extractive series of 10 are 20.3, 23.6, and 22.6 per thousand, while those of the aromatic oxygen are 2.3, 0.5, and -0.5 per thousand. In the synthetic sample, the values are 12.6 and 5.6 per thousand, respectively. As a final product, the reduction of 10 leads to the dihydroderivative 11. Both the baker's yeast reduction and the catalytic hydrogenation lead to a marked decrease of the deuterium content of 11, which is stronger for the beta-position than for the alpha-position.     

Stable isotope labeling pattern of resveratrol and related natural stilbenes

The stable isotope characterization of resveratrol 1 from Polygonum cuspidatum and of related natural stilbenes 11 and 12 obtained by hydrolysis of the corresponding glucosides 2 and 3 from Rheum is reported. The C(6)-C(2)-C(6) framework of suitably protected derivatives of 1, 2, and 3 has been degraded with ozone to the C(6)-C(1) aldehydes 4, 5, 9, and 10, retaining all hydrogen atoms of the precursors. The natural and synthetic derivatives are characterized and distinguished by natural abundance deuterium nuclear magnetic resonance studies. In the case of anisaldehyde 4 the two series show, as expected, the characteristic difference of the aromatic labeling. The formyl deuterium contents of 4 and 5 from resveratrol are remarkably different, seemingly reflecting the different enrichments existing between positions 3 and 2, respectively, of the phenylpropanoid precursor. The positional delta(18)O values of the extractive materials 1-3 were also determined. In this instance a selective deoxygenation procedure was adopted, leading from 1 to the products 6, 7, and 8. The delta(18)O values of the latter compounds reveal, respectively, those at position 4' and positions 3 and 5 of 1. Similarly, the phenolic products 11 and 12 were converted into 13 and 14. From the delta(18)O values of the single components it is possible to design a detailed map of the oxygen fractionations which characterizes the stilbenes 1-3. In particular, the oxygen present at position 4' of the phenylpropanoid moiety of 1-3 shows delta(18)O values of +11.5, +1.8, and +6.7 per thousand, respectively. Moreover, the phenolic oxygen atom at position 3' of rhapontin 3 shows a value of +11.7 per thousand. The data are compared with those previously obtained on structurally related compounds. These results show the utility of simple chemical degradations in the stable isotope characterization of structurally complex food components.     

CropSyst, a cropping systems simulation model

CropSyst is a multi-year, multi-crop, daily time step cropping systems simulation model developed to serve as an analytical tool to study the effect of climate, soils, and management on cropping systems productivity and the environment. CropSyst simulates the soil water and nitrogen budgets, crop growth and development, crop yield, residue production and decomposition, soil erosion by water, and salinity. The development of CropSyst started in the early 1990s, evolving to a suite of programs including a cropping systems simulator (CropSyst), a weather generator (ClimGen), GIS-CropSyst cooperator program (ArcCS), a watershed model (CropSyst Watershed), and several miscellaneous utility programs. CropSyst and associated programs can be downloaded free of charge over the Internet. One key feature of CropSyst is the implementation of a generic crop simulator that enables the simulation of both yearly and multi-year crops and crop rotations via a single set of parameters. Simulations can last a fraction of a year to hundreds of years. The model has been evaluated in many world locations by comparing model estimates to data collected in field experiments. CropSyst has been applied to perform risk and economic analyses of scenarios involving different cropping systems, management options, and soil and climatic conditions. An extensive list of references related to model development, evaluation, and application is provided.     

Feeding of yeast(Candida spp.) improves in vitro ruminal fermentation of fibrous substrates

In vitro gas production technique(IVGPT) was used with the objective of determining the inclusion effect of live cells of two strains of Candida yeast on in vitro ruminal fermentation of two fibrous substrates. In order to achieve this, two experiments were performed: A) using oat straw(Avena sativa) as substrate; B) using alfalfa hay(Medicabo sativa) as substrate, comparing the effect of two different strains of Candida genre, both isolated from the rumen, on the mentioned substrates. Levica 25(Candida tropicalis) yeast belongs to the culture collection of the Institute of Animal Science, Cuba, and Levazoot 15(Candida norvegensis) yeast is part of the collection of the Faculty of Zootechnology and Ecology of the Autonomous University of Chihuahua, Mexico. Both strains demonstrated their potential in activating the ruminal fermentation. They stimulated(P<0.0001) the ruminal fermentation of the substrates under study. However, the Levazoot strain stimulated the dry matter(DM) fermentation of alfalfa in 21.43%, more than Levica 25. It is concluded that there is an influence of yeast strain and diet on the rumen environment and, therefore, it is important to select the appropriate strain in every production condition.     

Effect of water recirculation rate and initial stocking densities on competent larvae and survival of the Pacific oyster Crassostrea gigas in a recirculation aquaculture system

The present study evaluated the effect of initial stocking density and water recirculation rate on larval yield (percent of initially stocked larvae alive at the end of the experiment) and production of competent larvae (percent of initially stocked larvae alive at the end of the experiment retained on a sieve with mesh size 239 μm) of Crassostrea gigas in a recirculation aquaculture system (RAS). Different initial larval stocking densities (80, 160 and 320 larvae mL^?1) and water flow rates (100, 200 and 300 mL min^?1, totalling renewal rates of 60, 120 and 180 times day^?1 of water volume in culture tanks, respectively) were evaluated in 2.4-L tanks using a completely randomized design in a factorial scheme. The physicochemical parameters of the water (temperature, salinity, pH, dissolved oxygen and conductivity) were stable in all treatments during the experimental period. Our results demonstrated that the production of C. gigas larvae was feasible at the proposed densities. However, the water renewal rate affected both yield and competent larvae in the recirculation aquaculture system. Oyster cultures with densities of 160 larvae mL^?1 and flow of water of 300 mL min^?1 showed the best yield (89.34 ± 18.43%) and rate of competent larvae (84.09 ± 16.38%) and are therefore recommended with the aim of optimizing larvae cultivation.