皮下包埋两性霉素B对兔真菌性皮肤病治疗效果研究

兔真菌性皮肤病是一类传染性极强的人畜共患接触性皮肤病。试验选取体重相近,已感染真菌性皮肤病的吉戎兔15只,分为3组,每组5只,试验组Ⅰ为对照组,注射生理盐水,组Ⅱ和组Ⅲ皮下分别包埋5mg和10mg的两性霉素颗粒剂。给药前和给药后第10天、第20天和第30天分别称重,并观察测定兔皮毛生长及秃斑恢复情况。结果表明,试验组兔增重均高于对照组,差异显著(P<0.05),试验末期组II和组Ⅲ秃斑恢复分别达95%和90%。因此,采用皮下包埋的给药方式有效地延长了两性霉素B的药效时间,可有效治愈兔真菌性皮肤病。     

瘤胃液移植在反刍动物中的研究进展

瘤胃液移植是一种将健康供体动物的瘤胃液转移给受体动物的技术。目前,针对瘤胃液移植的报道还相对有限,但越来越多的研究结果显示,瘤胃液移植可以影响反刍动物的干物质采食量、产奶量等,还具有重建瘤胃菌群结构的潜力。本文综述了瘤胃液移植的方法及实践中的应用研究,分析其对反刍动物生长性能、瘤胃发酵、瘤胃微生物区系的影响以及在治疗某些疾病方面的作用,为反刍动物瘤胃液移植的研究提供参考。     

对处理一起市场上投诉的黄膘猪肉的体会

1事件回顾
　　2010年7月某日,某市某农贸市场有肉商向市场管理部门投诉,称其雇人在该市某县屠宰厂屠宰的猪为黄膘猪,请市场管理部门协助处理,并把猪胴体、内脏丢弃在市场管理办公室前的地上,引来群众围观。市场管理方当即向辖区的城区政府汇报;也有群众分别致电当地媒体和市政府热线,政府热线要求市水产畜牧兽医局处理,市水产畜牧兽医局安排市动物卫生监督所派员处理。     

牛胚胎移植技术的产业化研究

本文探讨了引进国外优质奶、肉牛冷冻胚胎建立核心群，以及供体牛选择、超数排卵、胚胎冷冻保存和分割等相关技术，为牛胚胎移植产业化奠定基础。     

Characterization of antibody response to porcine reproductive and respiratory syndrome virus ORF5 product following infection and evaluation of its diagnostic use in pigs.

The sensitivity and specificity of recombinant open reading frame 5 products used in the Western blotting assay for confirmation of porcine reproductive and respiratory syndrome virus (PRRSV) serologic status were evaluated. The recombinant antigen-based assays were specifically compared with a commercial enzyme-linked immunosorbent assay (ELISA) for PRRSV antibodies using 1) PRRSV antibody-negative reference sera (n = 30), 2) naturally infected pig sera (n = 40), 3) sequential sera obtained from 24 experimentally infected pigs, and 4) sera submitted to 3 state diagnostic laboratories (n = 200). The recombinant antigen assay yielded an average increased sensitivity of 10% over the commercial PRRSV ELISA. The negative controls (group 1 sera) showed no difference between the 2 assays. This comparison confirmed that the recombinant antigen-specific assay was more sensitive than the commercial ELISA and is well suited for routine confirmation of the presence of PRRSV antibodies.     

通过器官病变诊断鸭病

一发病情况本县一养鹅专业户葛某饲养有220只浙东大白鹅的种鹅,2009年1月8日从邻近乡一鹅场购入苗鹅600只。购入一周后用禽流感疫苗注射0．5mL,     

萨福克公羊早期利用的研究

采用萨福克与湖羊杂交的方式,与配湖羊第一次使用萨福克公羔(6月龄),结果受胎率为18%(配38只母羊,产羔6胎);第二次使用萨福克公羔年龄为7月龄,精液品质同6月龄相比较有所提高;第三次试验在萨福克公羔8月龄以后进行,结果显示8月龄后公羔的精液品质有明显提高,精液品质已接近正常,同时给80只湖羊进行人工授精,受胎60只,受胎率80%,精液质量和受胎率都有较大的提高,同6月龄和7月龄相比,受胎羊数及受胎率差异极显著(P<0.01),240天其平均日增重250g。     

巴马小型猪在辽宁地区的适应性观察及血液生理生化指标检测

从广西巴马瑶族自治县引进巴马小型猪20头,进行饲养繁殖,观察其在东北环境下的适应性及血液生理生化指标的变化.经过2年的观察与检验,证实引进的巴马小型猪适应性良好,繁殖性能正常,性成熟期为2～3月龄,妊娠期为114～120 d,初产仔猪数为3～7头,经产仔猪数为5～10头,产仔猪数与母猪体重呈正相关(R²=0.96).血液生理生化指标检测结果显示,与巴马原种厂提供的数据相比,白细胞数(WBC)、血红蛋白(HGB)、天门冬氨酸氨基转移酶(AST)、尿素(Urea)、肌酐(Cr)、磷酸肌酸激酶(CK)、乳酸脱氢酶(LDH)、葡萄糖定量试验(GLU)、总胆固醇(T-Ch)、甘油三酯(TG)、胆碱酯酶(CHS)、Na、Cl、淀粉酶(AMS)和二氧化碳结合力(CO₂CP)均有显著差异(P<0.05),其余指标差异均不显著(P>0.05).结果表明由辽宁省饲养并繁殖的巴马小型猪的血液生理生化指标与人类具有一定的相似性,可以为辽宁地区试验应用巴马小型猪提供参考依据.     

浅谈高尔夫球场建造的成本控制

高尔夫球场建造项目经济利润是高尔夫施工单位存在和长期发展的基础,也是施工单位追求的最终目标.高尔夫施工单位项目利润主要来源于建造工程成本的控制.随着市场经济的深入发展,高尔夫项目建造竞争越来越激烈,施工单位的利润空间越来越低.通过有效措施来控制高尔夫球场建造的成本,成为施工单位获得最大盈利的重点.本研究通过阐述高尔夫球...     

Comparative pharmacokinetics and tissue distribution of quinocetone in crucian carp (Carassius auratus), common carp (Cyprinus carpio L.), and grass carp (Ctenopharyngodon idella) following the same experimental conditions

The pharmacokinetics and tissue distribution of quinocetone (QCT) in crucian carp (Carassius auratus), common carp (Cyprinus carpio L.), and grass carp (Ctenopharyngodon idella) were compared after oral administration of QCT (50 mg/kg body weight) at water temperature of 24 ± 1 °C. Similar QCT plasma concentration–time profiles were found in the three species of cyprinid fish at the same dosage regimen and water temperature, which were all fitted two‐compartment open pharmacokinetic model. However, different pharmacokinetic parameters were observed in crucian carp, common carp, and grass carp. The absorption rate constants (K_a) of QCT were 1.65, 1.40 and 1.74/h, respectively and absorption half‐lives (t_1/2kα) were 0.42, 0.49, and 0.40/h, respectively. The distribution half‐life (t_1/2α) was 2.83, 0.67, and 0.88 h, respectively, and elimination half‐lives (t_1/2β) of QCT were 133.97, 63.55, and 40.76 h, respectively. The maximum concentrations (C_max) of QCT in plasma were 0.315, 0.182, and 0.139 μg/mL and the time to peak concentrations (T_p) were 1.45, 0.96, and 1.08 h, respectively. The area under the plasma concentration‐time curves (AUC) were 12.35, 5.99, and 4.52 μg·h/mL, respectively. The distribution volumes (V_d/F) of QCT were calculated as 117.81, 128.71, and 220.10 L/kg, respectively. The tissue analysis showed that a similar regularity was obtained in the three species of cyprinids with a single dose of 50 mg/kg body weight after oral administration at the same water temperature. The tissue concentration of QCT in each fish was in order of liver>kidney>muscle, while the residues of QCT in the three species of cyprinid fish were in order of crucian carp>common carp>grass carp.