Observation of polymer conformation hysteresis in extensional flow

Highly extensible Escherichia coli DNA molecules in planar extensional flow were visualized in dilute solution by fluorescence microscopy. For a narrow range of flow strengths, the molecules were found in either a coiled or highly extended conformation, depending on the deformation history of the polymer. This conformation hysteresis persists for many polymer relaxation times and is due to conformation-dependent hydrodynamic forces. Polymer conformational free-energy landscapes were calculated from computer simulations and show two free-energy minima for flow strengths near the coil-stretch transition. Hysteresis cycles may directly influence bulk-solution stresses and the development of stress-strain relations for dilute polymer flows.     

角形区域的Teichmuler极值映照

采用二次微分的方法，得到了角形区域Ω１的Ａｆｆｉｎｅ变换关于其边界值不是极值映照．并明确给出在边界同伦下唯一极值的Ｔｅｉｃｈｍｕｌｅｒ映照．     

Isoflavone profile and biological activity of soy bread

The present study examines the ability of isoflavone extracts from whole soy bread and two soy bread fractions, crumb and crust, to modulate the proliferation of human prostate cancer PC-3 cells. Total isoflavone content in the two fractions of soy bread were similar (3.17 micromol/g dry basis). However, their conjugate patterns were altered. Both fractions of soy bread contained a similar level of isoflavone aglycones ( approximately 24%). Low concentrations of soy bread extracts increased PC-3 cell proliferation as much as 47% compared to untreated control. This proliferative effect in cell growth was reduced at higher extract concentration. Soy bread crust extract (10 mg/mL) reduced PC-3 cell proliferation by 15% compared to untreated control. Interestingly, wheat bread extracts increased cell proliferation at all concentrations tested. Although extracts from both breads possessed biological activity, only soy bread crust extract reduced PC-3 cell proliferation. This observation may be related to the presence of soy in this bread.     

西洋参韧皮坏死病与类菌原体

 西洋参(Panax quinquefolium)罹病表现为花变叶和韧皮坏死症状.根部韧皮组织超薄切片观察,发现大量具单位膜而无细胞壁,形态各异的类菌原体。存在于筛管分子及其周围伴胞中.类菌原体大小为90—950nm,外膜厚度8—9nm.以50单位/ml四环素三次喷洒植株地上部,能显著降低根部块死的病情指数.初步认为西洋参韧皮组织坏死,是由类菌原体引起的病害。     

水稻品种苗期阶段对白叶枯病的抗性研究

 根据水稻品种各叶期对白叶枯病的抗性表现和强弱程度可分为四类:(1)全期抗病型;(2)全期中抗型;(3)苗感成株抗病型;和(4)全期感病型。可以看出,水稻品种除少数苗感成抗型外,其余三种抗性类型苗期与成株期的抗性反应是一致的。由于中抗、成抗和感病品种各叶期的抗性有随着叶位的升高而抗性增强的趋势,不同抗性品种在苗期阶段抗感对比均较成株期明显。因此,水稻品种对白叶枯病的抗性鉴定采用苗期鉴定方法是可行的。影响水稻品种苗期抗性的因素是:苗龄(叶期)、观察时期、品种与菌株的组合等。     

The effect of cyclopiazonic acid on the development of pale, soft, and exudative pork from pigs of defined malignant hyperthermia genotype

Malignant hyperthermia (MH) and the mycotoxin cyclopiazonic acid (CPA) are each associated with abnormal calcium homeostasis in skeletal muscle, a key underlying factor in the development of pale, soft, and exudative (PSE) pork. To determine whether the natural presence of CPA in livestock feed ingredients contributes to the varying incidence of PSE in the pork industry, various levels of CPA (.1 to 50 mg/kg of diet) were included in the diets of market weight hogs (n = 52) of defined malignant hyperthermia genotype (NN = normal, Nn = a MH carrier, and nn = MH-positive). Animals with two copies of the MH mutation (nn) displayed improved live animal performance compared with NN and Nn animals (increased feed intake, average daily gain, and feed efficiency) but yielded lower quality loin chops as indicated by lower 45-min pH (P<.01), higher Commission Internationale de l'Eclairage (CIE) L* color coordinate values (P<.05), and higher drip losses (P<.01). The effects of CPA varied. In the first feeding trial, conducted under normal outside temperatures (2 degrees C), CPA had no effect (P> .2) on either live animal performance or meat quality. During the second trial, conducted under extreme outside temperatures (-18 degrees C), CPA-dependent reductions (P<.05) in feed intake, average daily gain, and 45-min pH in nn hogs support the possibility of interactions between malignant hyperthermia and dietary CPA on skeletal muscle calcium homeostasis and the development of PSE pork. These results suggest that this interaction may require stressful environmental conditions or the ingestion of CPA doses much higher than occur under natural conditions.     

北京栽培白蘑菇病害的研究

 1979~1980年进行箱式栽培白蘑菇的试验,并同时调查对比病害发生情况,和采用各种措施的效果。     

Effect of tumor infiltrating lymphocytes on the expression of MHC molecules in canine transmissible venereal tumor cells

Canine transmissible venereal tumor (CTVT) can be allo-transplanted across major histocompatibility complex barriers. The expression of MHC molecules is usually low in the progression (P) stage and then greatly increases during tumor regression (R). We investigated the effects of tumor infiltrating lymphocytes (TIL) on the expression of MHC molecules of CTVT cells. Isolated, viable CTVT cells were inoculated at each of 12 sites (1 x 10(8) CTVT cells per site) on the back of six, mixed-breed dogs. Tumor masses were collected every 2-3 weeks and prepared for histopathologic, immunocytochemistry, flow cytometry and immunoblotting studies. The level of MHC expression on tumor cells from different stages of growth was measured. Initially, expression of MHC I and II molecules in P phase CTVT was low. Twelve weeks post-inoculation (PI), expression increased dramatically and it continued to increase during R phase. Tumor growth slowed after 12 weeks PI and tumors entered R phase around 17 weeks PI. We hypothesize that CTVT evades host immunosurveillance and grows progressively for 12 weeks, when it becomes vulnerable and subject to the host's anti-tumor immune responses. We further demonstrated that R phase, but not P phase, TIL were closely associated with the over-expression of MHC I and II molecules by CTVT cells. The number and proportion of TIL were higher in R phase tumors. Supernatants, from R phase co-cultures (CTVT+TIL) and TIL only, promoted MHC I and II expression on P phase CTVT cells. After culturing alone for 1 month, expression of MHC classes I and II molecules in R phase CTVT cells decreased to the level of P phase CTVT cells. However, the above-mentioned supernatants restored their expression of MHC I and II molecules. In contrast, supernatants from P phase TIL or CTVT cells increased expression slightly or had no effect. Therefore, TIL, not CTVT cells, produce the effective substance (s) to promote the expression of MHC molecules by the tumor cells. Heat treated supernatant was unable to promote the expression of MHC I and II molecules by CTVT cells. In conclusion, TIL isolated from R phase CTVT secreted a heat-sensitive, soluble substance(s) that triggered over-expression of MHC I and II after 12 weeks PI. This caused the tumor to enter R phase and helped stop CTVT growth. Our findings will facilitate the understanding and further investigation of the mechanisms that initiate host immune surveillance against tumors.     

烟草气孔导度对光强的响应

气孔导度模型是评价植物叶片气孔调节的重要工具。为比较几种常用的气孔导度模型对烟草的适用性, 本研究采用Li-6400光合测定系统, 测定了大田条件下, 烟草叶片在控CO₂(390 μmol·mol^-1)和控温(20 ℃、25 ℃、30 ℃和35 ℃)情况下的气孔导度光响应曲线。以Ball-Berry模型(BB模型)、Leuning修正模型(BBL模型)、叶子飘和于强推导的机理模型(BBY模型), 对各温度下烟草的气孔导度进行拟合和比较, 将气孔导度模型与净光合速率的光响应修正模型进行耦合(耦合模型), 研究了烟草气孔导度的光响应特征, 并与Jarvis模型进行比较。拟合结果表明, 较之BB模型和BBL模型, BBY模型能更好地描述各温度下烟草气孔导度与净光合速率之间的关系。耦合模型和Jarvis模型都能较好地拟合烟草气孔导度对光强的响应曲线, 但耦合模型的拟合效果更好, 且可以直接估算最大气孔导度和对应的饱和光强, 同时可以研究最大气孔导度与最大净光合速率是否同步的问题。结果表明, 各温度下, 烟草最大气孔导度与最大净光合速率并不同步。20 ℃下气孔导度早于净光合速率达到最大值, 而其余温度下气孔导度晚于净光合速率达最大值。     

中国食蚜蝇亚科昆虫的区系分析（双翅目：食蚜蝇科）

本文对我国食蚜亚科昆虫的区系进行了分析。我国该亚科昆虫的区系具备以下特点：东洋区与古北区种类所占比重较大，两者分别占总种数的３０．１０％和２８．０６％，几乎相等；而特有种和广布种较为丰富；以食蚜蝇族的区系最具多样性。