3品种工作犬幼犬警用行为测试

通过测试3品种工作犬幼犬的警用行为表现,为深入研究工作犬的品种特性,改进受训犬考评机制、提高训练的技术手段及有效建设种犬群提供数据支撑和理论依据.随机选取德国牧羊犬、昆明犬、马里努阿犬的幼犬,共计254头,测定其胆量、兴奋性及衔取等警用行为参数,以品种、品种间公母及本品种公母分组进行对比分析.结果显示,德国牧羊犬、昆明...     

EOS/MODIS遥感监测在甘肃迭部重大森林火灾中的应用

森林生态系统是陆地自然生态系统中最大的生态系统,它在整个自然界的动态平衡中具有重要的作用.森林火灾是生态环境的一种重大自然灾害,全世界每年发生森林火灾几十万次.特别是20世纪80年代以来,全球气候持续变暖,林火有上升的趋势(舒立福等,1997).     

沙棘木蠹蛾综合控制技术研究

沙棘木蠹蛾是近几年在我国内蒙古、辽宁、山西、宁夏、陕西和甘肃等地大面积成灾的一种钻蛀性害虫,主要以幼虫危害沙棘的根部和干部.由于该虫以前从未大面积发生危害,相关的研究报道较少.笔者从沙棘木蠹蛾的成灾原因、生物生态学特性、可供监测和防治的性引诱剂,以及天敌种类和自然控制效果等方面进行了较全面的研究,取得了重要研究成果.     

野生花卉多花胡枝子种子萌发特性

多花胡枝子(Lespedeza floribunda Bunge.)属豆科胡枝子属(Lespedeza)多年生落叶小灌木,花冠紫色、紫红色或蓝紫色,花色艳丽,花期(6-9月)较长,且花期正值春花凋零,夏花不多之际,可作为良好的晚春及夏季观花灌木[1].除观赏价值外,多花胡枝子枝条细软,适口性良好,具有良好的饲用价值[2].     

A combined application of molecular docking technology and indirect ELISA for the serodiagnosis of bovine tuberculosis

BackgroundThere is an urgent need to find reliable and rapid bovine tuberculosis (bTB) diagnostics in response to the rising prevalence of bTB worldwide. Toll-like receptor 2 (TLR2) recognizes components of bTB and initiates antigen-presenting cells to mediate humoral immunity. Evaluating the affinity of antigens with TLR2 can form the basis of a new method for the diagnosis of bTB based on humoral immunity.ObjectivesTo develop a reliable and rapid strategy to improve diagnostic tools for bTB.MethodsIn this study, we expressed and purified the sixteen bTB-specific recombinant proteins in Escherichia coli. The two antigenic proteins, MPT70 and MPT83, which were most valuable for serological diagnosis of bTB were screened. Molecular docking technology was used to analyze the affinity of MPT70, MPT83, dominant epitope peptide of MPT70 (M1), and dominant epitope peptide MPT83 (M2) with TLR2, combined with the detection results of enzyme-linked immunosorbent assay to evaluate the molecular docking effect.ResultsThe results showed that interaction surface Cα-atom root mean square deviation of proteins (M1, M2, MPT70, MPT83)-TLR2 protein are less than 2.5 A, showing a high affinity. It is verified by clinical serum samples that MPT70, MPT83, MPT70-MPT83 showed good diagnostic potential for the detection of anti-bTB IgG and M1, M2 can replace the whole protein as the detection antigen.ConclusionsMolecular docking to evaluate the affinity of bTB protein and TLR2 combined with ELISA provides new insights for the diagnosis of bTB.     

日本北海道北部寒温带森林流域林冠层凋落物及其养分归还量的景观格局(英文)

Within a forested watershed at the Uryu Experimental Forest of Hokkaido University in northern Hokkaido, overstory litterfall and related nutrient fluxes were measured at different landscape zones over two years. The wetland zone covered with Picea glehnii pure stand. The riparian zone was deciduous broad-leaved stand dominated by Alnus hirsuta and Salix spp., while the mixture of deciduous broadleaf and evergreen conifer dominated by Betula platyphylla, Quercus crispula and Abies sachalinensis distributed on the upland zone. Annual litterfall averaged 1444, 5122, and 4123 kg.hm^-2·a^-1 in the wetland, riparian and upland zones, respectively. Litterfall production peaked in September-October, and foliage litter contributed the greatest amount （73.4%-87.6 %） of the annual total litterfall. Concentrations of nutrients analyzed in foliage litter of the dominant species showed a similar seasonal variation over the year except for N in P glehnii and A. hirsuta. The nutrient fluxes for all elements analyzed were greatest on riparian zone and lowest in wetland zone. Nutrient fluxes via litterfall followed the decreasing sequence： N （11-129 kg.hm-2.aq） 〉 Ca （9-69） 〉 K （5-20） 〉 Mg （3-15） 〉 P （0.4-4.7） for all stands. Significant differences were found in litterfall production and nutrient fluxes among the different landscape components. There existed significant differences in soil chemistry between the different landscape zones. The consistently low soil C：N ratios at the riparian zone might be due to the higher-quality litter inputs （largely N-fixing alder）.     

大麦开颖性状的遗传分析Ⅰ.大麦开颖性状的遗传效应和遗传模型

为了研究大麦开颖性状的遗传规律,以5个开颖品种（系）（黄长芒大麦、沪1154、1430R、99-7122、扬0187）和1个闭颖品种（91单2）为亲本,采用6^2完全双列设计,配制杂种F1．开花期利用图象处理软件Adobe photoshop测量亲本及杂种F1的开颖角度,并对所测的数据进行方差分析．同时计算公共亲本的系列方差Vr和系列协方差Wr,以测验开颖性状的遗传模型．推断其显（隐）性基因频率分布特点。结果表明：（1）亲本间和杂种间的F值分别为237．71^＊＊和162．48^＊＊,表明开颖角度在亲本间和杂种间的差异均这到极显著水平;（2）Wr依Vr的回归方程为Wr=4,5128＋0．8968Vr,r=0＋9339^＊＊,回归系数与0的差异（t=5．23^＊＊,df=4）达到极显著水平,与1的差异（t=-0．60,df=4）不显著,即开颖性状的遗传符合加性-显性模型;回归截距与0的差异（t=0．86,df=4）也不显著,即开颖性状的遗传属完全显性类型;加性、显性和环境方差的估计数依次为45．922、20．321和1．210,说明加性遗传方差比非加性遗传方差更重要;开颖显性方向和程度随亲本和组合而有所不同,平均而言．开颖性状是减效基因表现为显性,即闭颖对开颖表现为显性;（3）15对正反交杂种F1中有3对的开颖角度存在正反交差异,表明大麦开颖性状有时还受到细胞质基因的影响。     

应用热扩散技术对油松栓皮栎比导率的研究

2001年4-10月,应用热扩散技术研究北京西山地区31年生油松栓皮栎混交林标准木的比导率并对13个环境因子的变化规律进行同步观测.结果表明,在野外自然条件下,油松和栓皮栎的比导率在标准日内均呈明显的凸形变化规律,栓皮栎的比导率始终高于油松.影响树木比导率的因素基本上可以分为2类,即生物学结构的遗传特性和环境因素.在林分生长期内的不同时段,环境因子对2个树种比导率的作用各不相同,即使是同一树种,在各个时段内对其比导率产生显著影响的环境因子也不是一成不变的,但一般可用少数几个环境影响因子来比较准确地模拟出比导率的变化.     

臭椿、新疆杨混交林对光肩星天牛选择寄主行为的影响

利用3年生臭椿、新疆杨在室内建立混交林进行光肩星天牛成虫放飞试验.结果表明:臭椿、新疆杨混交林对天牛成虫的寄主选择有显著影响,天牛成虫的交尾行为只在新疆杨植株上进行;天牛成虫在林内植株上着落的总虫次随新疆杨株数的减少而下降,交尾次数也减少;臭椿、新疆杨植株数量在林分中所占比例在一定范围时,天牛成虫在新疆杨和臭椿上着落的虫次比均趋于稳定;新疆杨与臭椿以行状3:2和2:3混交时林分的抗虫效果较好.     

Computationally Assisted Structural Elucidation of Cembranoids from the Soft Coral Sarcophyton tortuosum

A persistent study on soft coral Sarcophyton tortuosum resulted in the characterization of two new cembranolides, tortuolides A and B (1 and 2), and a new related diterpene, epi-sarcophytonolide Q. Their structures were determined not only by extensive spectroscopic analysis but also by DFT calculations of ECD and NMR data, the latter of which was combined with statistical analysis methods, e.g., DP4+ and J-DP4 approaches. Anti-inflammatory and cytotoxicity activities were evaluated in this study.