苹果果实表皮总蛋白提取及双向电泳方法的优化

以苹果果实表皮为试材,采用3种方法提取果实表皮总蛋白,并对双向电泳(2-DE)操作体系进行优化。结果表明:改良酚提法适于苹果果实表皮总蛋白的提取,优化后的2-DE分离体系为预制IPG胶条18cm pH 4~7,上样量600μg,改进后的等电聚焦程序,浓度16%的分离胶,适于苹果果实表皮总蛋白的2-DE分离。     

弱光胁迫对七个辣椒组合的生长发育及产量的影响

以驻马店市农业科学院园艺所近年来培育的7个辣椒组合(品种)为试材,利用遮阳网模拟弱光胁迫,调查了在弱光胁迫下辣椒植物学性状及产量性状,以株高、茎粗作为参考指标,以叶面积、比叶干质量作为重要指标,以产量、叶绿素a/b值作为主要指标,对其弱光耐受性进行了综合评价。结果表明:弱光逆境胁迫导致辣椒植株增高、茎秆变细、叶面积增大、比叶干质量降低、产量及叶绿素a/b值均降低;且叶绿素a/b值与产量的变化幅度基本一致,该方法可用来鉴定辣椒不同组合的耐弱光性能。     

利用SSR标记构建贵州山区玉米杂交种的指纹库

对贵州山区目前在生产上正在使用的西山系列玉米杂交种12份进行了SSR分析。从60对引物中筛选到稳定性好、多态性丰富的8对引物作为核心引物。用这8对引物的指纹组合构建了贵州山区西山系列玉米杂交种的DNA指纹数据库。结果表明,利用SSR技术进行玉米杂交种真实性的鉴定是可行、有效的。     

Nonlinear Characteristic Modeling of Servo Value on Hydraulic Bending Roll System''s Dynamic Characteristic

Nonlinear characteristic of servo value is the important factor influencing hydraulic system, the model of electro-hydraulic servo pressure control system is designed. In this model, flux-equation of servo valve is specially applied and some non-linear characteristics are considered. Furthermore, SIMULINK toolbox of MATLAB language software is applied to solve mathematic model and simulation curves of some variables are obtained. The calculation results are in good agreement with the experimental results.     

Reuolution and Research of Green Automobile

The green is as a symbol of no pollution,no public harm and environmental protection. The green automobile is the inevitable results that the automobile technique continuously developes. A new style vehicle--the green automobile is the urgent solicit of environmental protection is developed. The automobile industry can develop continuously. This article mainly discusses the importance of the green auto_mobile's research and development,the development status in quo and prospect of the green auto_mobile technique in the world,and states the relationship between the green automobile technique and environmental protection from green technical system theoretically clarify the study of green automobile,including its design and the system,the manufacture and its technology,the energy,the rebuild engineering and a series of subjects. How to evaluate the cycle and the evaluable mark of the green automobile,the prospect of green automobile are pointed out.     

Third-party Logistics Subcontract Design Based on Bilevel Programming

The research theme of this paper is about how third-party logistics provider design a kind of effective incentive mechanism which can not only avoid the moral hazard behavior of the subcontractor , but also promote subcontractor to raise the serving performance constantly, until obtaining the best TPL performance .Through building bilevel programming model where constructing the Nash equilibrium model this paper, the anthors assume subcontract decision variable as endogenetic variable first, and then analyze the optimal solution with the restraint of Nash equilibrium at the time of decision variable as exogenous variable, push the subcontract relationship research at the principal-agent level into the lay that principal-agent combining with whole contract performance optimal decision.     

Preparation of Electrochromic and Transparent Film of Polyaniline

The preparation method of transparent film of electrochromism polyaniline on the glass supporter is studied by a dipping polymerization. The electric and optic properities of the resulting film are measured as a function of oxidant,dopant,reaction time and temperature.The optimum technology term is obtained in the normal atmospheric temperature.The experimental result is testified by fuzzy set theory.     

The Public Participation in the Urban Planning

The policy of public participation in urban planning was first practiced in Europe in the 1960s. After 40 years of development, the policy has become the essential step of city planning in Western countries where as is just at the elementary step in China. For the purpose of knowing the situation about the policy performing in local cities and discussing the feasibility of public participation in urban planning, several kinds of investigation have been done. From the investigation, it is found that although the public participation has already been the important step of urban planning in some city during just several years,there are still many problems. This paper shows a panorama view of this issue. Th describe the future of public participation in e authors will offer some solutions according to the problems and the urban planning.     

南方涝渍地综合整治开发效果的评价研究

以 2个示范区作为实例构建了一套对南方涝渍地实施以农业工程技术为基础的综合整治与建设后的效果进行评价的评价体系。该体系由工程效果、农业综合开发效果和综合效益等 3部分构成。在工程效果评价中 ,重点对工程实施后的抗灾减灾效果和代表经过改良后农田物理构架改变的“农田综合生产力指数 (IPI)”等二方面进行了分析。研究表明 ,该评价体系能比较全面、有重点地对涝渍地综合改良的实效做出评价。由于该体系实现了评价的数量化 ,使用该评价体系既利于对同一地区改良前后的效果做出对比分析、找出存在的问题 ,也利于对邻近不同地区的改良效果做出比较评价。对 2个示范区进行实际评价的结果表明 ,该地区的涝渍地综合整治开发效果良好 ,其第一期工程取得成功。     

棉田膜内与膜间土壤溶液含盐量的变化

用定期取表层土样测定其电导率和在不同深度埋设盐分传感器探头的方法 ,在作物生长季节内 ,监测了棉田膜内和膜间不同深度的土壤溶液含盐量变化。结果为 :1棉田膜内与膜间表层土壤和 1 0 cm、30 cm深土壤溶液含盐量变化较大 ,且规律相同。未灌水时上升 ,灌水后迅速下降。膜间未灌水期间的含盐量上升幅度大于膜内 ,灌水对膜内和膜间表土洗盐、1 0 cm、30 cm深土壤溶液含盐量的降低作用没有差别。降雨可引起膜内和膜间 1 0 cm深土壤溶液电导率的变化 ,这个影响也是膜间大于膜内。 2 60 cm深的土壤溶液不存在明显的盐分累积现象 ,灌水可使这里的土壤溶液含盐量缓慢降低 ,膜间的降低过程快于膜内