Fleets of robots for environmentally-safe pest control in agriculture

Feeding the growing global population requires an annual increase in food production. This requirement suggests an increase in the use of pesticides, which represents an unsustainable chemical load for the environment. To reduce pesticide input and preserve the environment while maintaining the necessary level of food production, the efficiency of relevant processes must be drastically improved. Within this context, this research strived to design, develop, test and assess a new generation of automatic and robotic systems for effective weed and pest control aimed at diminishing the use of agricultural chemical inputs, increasing crop quality and improving the health and safety of production operators. To achieve this overall objective, a fleet of heterogeneous ground and aerial robots was developed and equipped with innovative sensors, enhanced end-effectors and improved decision control algorithms to cover a large variety of agricultural situations. This article describes the scientific and technical objectives, challenges and outcomes achieved in three common crops.     

Effect of Pseudomonas putida and inorganic fertilizer on growth and productivity of habanero pepper (Capsicum Chinense Jacq.) in greenhouse

The habanero pepper is widely used in a variety of commercial food products. The increase in the cultivation area of the habanero pepper has increased the use of agricultural inputs, mainly inorganic fertilizers, which have high economic costs and cause negative effects to the soil and water. In recent years, the use of beneficial soil microorganisms has been studied as an alternative to inorganic fertilizers. In the present study, the effect of three strains of Pseudomonas putida on the growth and productivity of King Pakal hybrid habanero pepper with limited inorganic fertilizer in a greenhouse. It was found that a reduction of inorganic fertilizer application of 25% with P. putida rhizobacteria strains increased or maintained growth and productivity of King Pakal hybrid habanero pepper. Before these rhizobacteria can be used in the field, results will first need to be confirmed under more natural conditions.     

Model legumes contribute to faba bean breeding

Faba bean is an excellent candidate crop to provide nitrogen input into temperate agricultural systems. However, its growth is hampered by several factors including environmental stresses and the presence of anti-nutritional factors. To solve these limitations, breeding programs have been initiated that were successful for monogenic traits but not so for multigenic traits. The large genome size of faba bean has slowed down breeding processes. Several other legumes have emerged as model legumes including Medicago truncatula, Lotus japonicus, Glycine max and Pisum sativum. The establishment of these models has already boosted our understanding of important processes such as the nitrogen-fixing symbiotic interaction. The high level of synteny and collinearity existing between legumes makes possible the transfer of key knowledge from model legumes to faba bean. Here we review the most recent knowledge gained from model legumes on grain quality, resistance to biotic and abiotic stresses, nitrogen-fixing symbiosis and how this knowledge can be employed for faba bean breeding.     

Bacterial community of pond's water,sediment and in the guts of tilapia (Oreochromis niloticus) juveniles characterized by fluorescent in situ hybridization technique

Information about bacterial community structure and functioning in fish farming ponds remains scarce, mainly due to methodological difficulties in counting and identifying uncultured bacteria. The main objective of this study was to evaluate the degree of similarity between the bacterial community of the digestive tract of tilapia (Oreochromis niloticus) juveniles and that of the test pond's water and sediment, using the Fluorescent In Situ Hybridization (FISH) technique. Samples of water, sediment and gut content of 30 tilapia juveniles from a single nursery ground were collected in January 2010. Potentially probiotic and pathogenic bacteria of the species Bacillus, Lactobacillus brevis, Lactobacillus collinoides, Lactobacillus coryniformis, Lactobacillus farciminis, Vibrio and Pseudomonas fluorescens were found in different samples using specific fluorescent probes. The similarity between bacterial community environments and gastrointestinal tracts was determined using the Morisita‐Horn index. The fish guts presented higher abundances of Pseudomonas fluorescens, Bacillus, Lactobacillus brevis and Lactobacillus collinoides. The bacterial community composition of tilapia gastrointestinal tract was more similar to the water than the sediment of the pond. The results of this study showed that the FISH technique can be easily used for monitoring of probiotics and pathogen detection in aquaculture systems.     

Nitric oxide reduces oxidative stress generated by lactofen in soybean plants

Nitric oxide (NO) is a molecule able to directly scavenge ROS and end chain reactions, which can be generated by some herbicides. This study aimed to evaluate whether the pretreatment of soybean plants with sodium nitroprusside (SNP) solution, a NO-donor substance, provides protection against oxidative stress generated by lactofen. Soybean plants were pretreated with SNP before lactofen application. The levels of lipoperoxides and photosynthetic pigments were quantified, and the activity of the antioxidant enzymes glutathione S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) was assessed. Although lipid peroxidation was not completely prevented, NO was able to scavenge ROS generated by the lactofen action, avoiding the photosynthetic pigment breakdown. Consequently, ROS scavenging by NO leads to a decrease in the available substrate for the antioxidant enzymes SOD, CAT, and POD, which are essential to protect plants under oxidative stress situations such as absence of GST induction by H₂O₂.     

Leaf Traits as Functional Descriptors of the Intensity of Continuous Grazing in Native Grasslands in the South of Brazil

Plant functional types (PFT) have been used to describe the response of native vegetation to environmental factors (i.e., fertility) and to livestock disturbance, but rarely under conditions of continuous grazing. In this work we investigate whether the long-term response of grassland communities submitted to a gradient of continuous grazing pressure can be described with such an approach. After 15 yr of differentiation of the grazing pressure applied to native grasslands we measured leaf dry-matter content (LDMC) and specific leaf area (SLA) of Poaceae populations of the communities. A grazing pressure gradient was created by levels of daily forage allowance: 4, 8, 12, and 16 kg of dry matter per day per 100 kg of animal live weight, monitored monthly. PFTs were defined by numerical analysis, where an algorithm finds the optimal trait subset based on the agreement between matrices of species × traits, paddocks × grass biomass, and environmental variables (levels of forage allowance and soil characteristics). The results show that it is possible to describe a gradient of grazing pressure by means of LDMC and/or SLA measured only on the Poacea contributing at least 80% of the total Poaceae biomass. Four PFTs were differentiated by these leaf traits. PFTs having low LDMC and high SLA are characteristic of high intensity of use and are made up largely of stoloniferous C₄ species typical of rapid resource capture strategies. Conversely, PFTs characterized by high LDMC and low SLA include species that are representative of low grazing pressure. Variations in the aggregate value of traits are due to changes in the species proportions and not to leaf-size adaptation as hypothesized. We conclude than in the absence of a gradient of fertility, plants with strategies of resource capture tend to be more represented under high grazing pressures. This situation results in a loss of functional diversity, but in particular a reduction in forage availability, which is incompatible with high animal production.     

Color preference,seasonality, spatial distribution and species composition of thrips (Thysanoptera: Thripidae) in northern highbush blueberries

We investigated color preference, seasonal abundance, spatial distribution and species composition of thrips in northern highbush blueberries, Vaccinium corymbosum L., in New Jersey (USA). White sticky traps were more attractive to thrips compared with yellow or blue traps. Thrips captures using white sticky traps showed that their flight activity begins 20–30 d after the onset of flowering, with 10, 50 and 90% of trap captures observed at 383, 647 and 1231 degree-day accumulations, respectively (10 °C base temperature). Two methods were used to study thrips distribution within a blueberry bush. First, white sticky traps were placed within the bush canopy at three different heights. The highest numbers of thrips were caught on traps in the middle and top one-third of the canopy while the lowest numbers were caught in the bottom one-third. A second method determined the distribution of thrips on the blueberry plant at different heights and phenological stages. The highest numbers of thrips were found on young leaves at lower parts of the canopy, whereas flowers and fruit had fewer thrips and none were found on buds; these thrips were identified as, Scirtothrips ruthveni (88% of adults) and Frankliniella tritici (12%). The distribution of thrips within a blueberry planting was investigated using an evenly-spaced grid of white sticky traps in combination with on bush beating-tray samples. Thrips counts from traps correlated with direct counts on the bush across the entire blueberry field (macro-scale level); however, within the field (micro-scale level), there was no correlation between the number of thrips on traps and on individual bushes near traps. Early in the season, trap counts were higher on bushes closer to the forest, indicative of movement of thrips from wild hosts into blueberry fields. However, this was not the case for direct on bush counts or trap counts for the later part of the season, where there was no clear forest “edge” effect. Percent fruit injury due to thrips feeding was low, and it correlated with thrips counts on bushes but not from counts on traps. Overall, our data show that thrips counts on sticky traps need to be interpreted with care because these numbers weakly correlated with the numbers of thrips on bushes at the micro-scale level and percent fruit injury; however, they can be useful predictors of thrips activity across entire blueberry fields (macro-scale).     

Improving the screening process for the selection of potato breeding lines with enhanced polyphenolics content

Efficient selection of potato varieties with enhanced nutritional quality requires simple, rapid, accurate, and cost-effective assays to obtain tuber chemical composition information. Our objective was to develop simple protocols to determine phenolics, anthocyanins, and antioxidant capacity in polyphenolic extracts of potatoes using Fourier transform infrared spectroscopy combined with multivariate techniques. Lyophilized potato samples (23) were analyzed. Polyphenolic compounds were extracted from potatoes and applied directly applied onto a three-bounce ZnSe crystal for attenuated total reflectance measurements in the infrared region of 4000 to 700 cm (-1). Robust models were generated (r > or = 0.99) with standard error of cross-validation values of 4.17 mg gallic acid equivalent/100 g (total phenolics), 0.87 mg pelargonidin-3-glucoside/100 g (monomeric anthocyanins), and 130.8 mumol Trolox equivalent/100 g (antioxidant capacity) potato powder. In addition, classification models discriminated potato samples at the species and variety level. Application of a simple infrared spectroscopic protocol allowed simultaneous rapid quantification of specific nutritional components in potatoes and efficient selection of value-added potato varieties.     

Capsicum annuum var. annuum under macronutrients and boron deficiencies: Leaf content and visual symptoms

This study aims to describe macronutrient and boron deficiencies in pepper grown in a hydroponic system in the presence of a substrate during the vegetative and fruiting phases. The experiments were carried out into a greenhouse, and consisted of a randomized block design with eight treatments: complete solution (control) and nitrogen (-N), phosphorus (-P), potassium (-K), calcium (-Ca), magnesium (-Mg), sulfur (-S) and boron (-B) deficient solutions, with four repetitions for each assay. Visual diagnoses of certain macronutrient and boron deficiencies can be applied by farmers to aid in soil management, since they are observed in leaves, while N, P, Ca, S and B deficiencies led to roots symptoms. Pepper fruits showed nutritional deficiency symptoms when grown under N, K, Ca, Mg, S and B deficits. The appearance of nutrient deficiency symptoms in pepper plants occurs in the following order: N, K, P, Mg, S, Ca and B.