Social behaviour of buffalo heifers during the establishment of a dominance hierarchy

This study investigated the social behaviour of buffalo heifers during the establishment of a dominance hierarchy under conditions that differed in space availability (pen, small pasture and large pasture), food competition and in the presence or absence of an intact bull. The herd consisted of 21 Mediterranean–Murrah crossbreed buffalo heifers and one bull. During the establishment of dominance hierarchy, one observer recorded all of agonistic interactions occurring in the pen, in the small pasture and in the large pasture. For the food competition test, the females were first separated into groups (G) of seven animals and the behavioural interactions within these groups were observed. The group levels were determined by the sociometric parameters (h′: Landau linearity index and DCI: directional consistency index). The percentage of unknown, one-way and two-way dyadic relationships; a social index, hierarchical position and correlations among feeding duration, latency, social index and hierarchical position were calculated. In the large pasture, the DCI value was 55.24% of the dyadic relationships were one-way and 36.19% were unknown; whereas in the small pasture and pen most of the dyads were unknown (54–63%) or one-way (33-41%). The social indexes observed were low (0.004 and 0.122). In the food competition test, h′ values were not significant for groups G1, G2, G3 and G6, and significant for Groups G4 and G5. The percentages of two-way dyadic relationships were high in G4 (80.95%) and G6 (100%) groups. Correlations among feeding duration and latency, social position and social index were all negative and significant. There were no significant correlations between body weight and social index or hierarchy position. This study showed that buffalo heifers established social dominance relationships among several of the herd members, but because there were several unknown dyads, the hierarchy is classified as semi-linear hierarchy. Two factors seemed to affect the establishment of social dominance and a hierarchy: adjusting to a new environment and the presence of an adult intact male, and the dominant females had advantage over the subordinate females during feeding in a trough. To reduce agonistic encounters between buffalo heifers suggest provide more space in the trough during feeding.     

Seasonal changes in plasma concentrations of cecum-derived amines in clinically normal ponies and ponies predisposed to laminitis

OBJECTIVE: To measure concentrations of amines formed in the cecum of clinically normal ponies, determine amine concentrations in plasma samples collected in spring and winter, and compare concentrations of amines and serotonin in plasma samples obtained from clinically normal ponies and ponies predisposed to laminitis. SAMPLE POPULATION: Cecal contents obtained from 10 ponies euthanatized at an abattoir and blood samples obtained from 42 adult ponies. PROCEDURE: Cecal contents were assayed for amines by high-performance liquid chromatography (HPLC). Blood samples were collected at various times of the year from 20 ponies predisposed to acute laminitis and 22 clinically normal ponies. Plasma serotonin concentration was measured by HPLC, and tryptamine (TRP), tyramine (TYR), phenylethylamine (PEA), and isoamylamine (IAA) were measured by liquid chromatography-mass spectrometry. RESULTS: 15 amines were identified in cecal contents. Plasma TRP, TYR, PEA, and IAA concentrations ranged from 10pM to 100nM in both groups of ponies. Plasma concentrations of serotonin or other amines did not differ between clinically normal ponies and those predisposed to laminitis; however, significantly higher concentrations of TRP, PEA, and IAA were found in samples obtained in the spring, compared with winter samples. CONCLUSIONS AND CLINICAL RELEVANCE: Various amines are found in the cecum of ponies, several of which can be detected in the plasma. Concentrations increase significantly in the spring and may reach concentrations close to the threshold for causing vasoconstriction. Release of amines from the cecum into the systemic circulation may contribute to hemodynamic disturbances in horses and ponies with acute laminitis.     

Digital blood flow and plasma endothelin concentration in clinically endotoxemic horses

OBJECTIVE: To measure plasma endothelin-1 (ET-1) concentrations and digital blood flow in clinically endotoxemic horses. ANIMALS: 36 adult horses that underwent emergency celiotomy for primary gastrointestinal tract disease. PROCEDURE: On days 2 and 5 following surgery, Doppler ultrasonographic digital arterial blood flow measurements were obtained. Hematologic and biochemical analyses were performed, and plasma concentrations of ET-1 and endotoxin (lipopolysaccharide) were determined. A scoring system based on 9 clinical variables was used to assign horses to group B (quartile with greatest cumulative score) or group A (remaining 3 quartiles). Follow-up at 2.5 years was obtained by telephone questionnaire. RESULTS: For all horses on day 2, median (interquartile values) plasma ET-1 concentrations were 1.4 (0.8, 1.7) pg/mL, whereas on day 5, plasma ET-1 concentrations were 1.0 (0.5, 1.6) pg/mL. On day 2, digital blood flow was 0.057 (0.02, 0.07) mL/min in group A horses and 0.035 (0.02, 0.03) mL/min in group B horses. On day 5, plasma ET-1 concentration was significantly (73%) higher in group B horses, compared with group A horses. Thirty of 36 horses were alive at 2.5 years; group A horses were more likely to have survived (odds ratio, 25; 95% confidence interval, 2.4 to 262). Significant associations were found between an increase in digital pulses, hoof wall temperatures, or both and increased digital blood flow (0.14 vs 0.04 mL/min) on day 2 and increased digital arterial diameter (0.32 vs 0.23 cm) on day 5. CONCLUSIONS AND CLINICAL RELEVANCE: Horses with more severe endotoxemia had decreased digital blood flow, increased plasma ET-1 concentrations, and decreased long-term survival.     

Contamination of dog hair with eggs of Toxocara canis

Toxocara canis, the common intestinal nematode of dogs and foxes, is the parasite responsible for human toxocarosis. It has recently been shown that dogs may harbour eggs of the parasite in their fur. To further investigate this claim a population of 100 stray dogs was examined to establish the prevalence and intensity of adult toxocaral worm infection in the intestines and eggs harboured in the hair. A novel method of washing the eggs from the hair was used. Sixty-seven percent of dogs were found to have T. canis eggs on their hair with a mean egg retrieval of nearly 584 eggs per gram from positive dogs. The age of the dog was found to be the only significant factor to influence the prevalence and intensity of eggs, with 95% of all the eggs recovered found on puppies. Thirty-nine percent of dogs were found to have adult T. canis worms in their intestine, although a significantly higher percentage of puppies (80%) were infected with worms than adults (22.5%). Puppies also had more worms per infection than adults and have a strong positive correlation between egg and worms numbers whereas adults did not. These studies show that stray dogs, particularly puppies, potentially harbour considerable numbers of eggs on their hair, at densities far higher than those reported in the soil or the general environment.     

Molecular characterization of H5N2 avian influenza viruses isolated from South African ostriches in 2006

Highly pathogenic avian influenza (HPAI) H5N2 reemerged in ostriches in South Africa during 2006, and a low-pathogenic AI H5N2 virus was also isolated. Molecular and phylogenetic characterization was performed to determine whether the outbreak strains were genetically derived from the supposedly eradicated Eastern Cape ostrich outbreak HPAI H5N2 strain of 2004. It was demonstrated that although the 2004 and 2006 South African H5N2 strains shared a common ancestor, the two outbreaks were not related. Not only were extensive reassortments with wild bird viruses involved in the evolution of the 2006 strains, but the precursor HA molecule HA0 cleavage site sequence of the 2006 HPAI H5N2 virus also contained fewer basic amino-acid insertions. Multiple transmission events occurred from wild birds to ostriches in 2006, and it appears that a reservoir of H5N2 with pathogenic potential for poultry is established in the South African wild duck population.     

Large‐scale Modeling of Soil Erosion with RUSLE for Conservationist Planning of Degraded Cultivated Brazilian Pastures

Pasture degradation is one of the major environmental and economic problems of Brazilian livestock production. Based on the estimates of soil loss in 140,297 km² pasturelands of Goiás State and the Federal District, the effects of land use and management and conservation practices on soil erosion by water were evaluated. Soil loss was estimated with the empirical revised universal soil loss equation model under four scenarios of land use and management of pastures and the implementation of terraces. The effects of converting hilly areas into permanently preserved areas were also evaluated. Well‐managed pastures planted in terraces and highly sloped areas with native vegetation featured a reduction of 709% in the rate of soil erosion by water in comparison with poorly managed pastures without soil conservation practices. The environmental consequences of pasture degradation become particularly important when they are associated with land use in the Amazon and Cerrado biomes because farmers in these regions are forced to open new natural areas. In addition, the high indices of bare soil in degraded pastures contribute significantly to sediment deposition in rivers and dams. The modeling of soil loss is demonstrated to be an important tool for land use planning and supporting public policies focused on the sustainable use of natural resources. The results of these simulations demonstrate the importance of actions to recover degraded pastures, such as the Federal Government's program known as ABC (Low Carbon Agriculture). Copyright © 2015 John Wiley & Sons, Ltd.