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991.
de Laat MA Sillence MN McGowan CM Pollitt CC 《Veterinary journal (London, England : 1997)》2012,191(3):317-322
Endocrinopathic laminitis is frequently associated with hyperinsulinaemia but the role of glucose in the pathogenesis of the disease has not been fully investigated. This study aimed to determine the endogenous insulin response to a quantity of glucose equivalent to that administered during a laminitis-inducing, euglycaemic, hyperinsulinaemic clamp, over 48 h in insulin-sensitive Standardbred racehorses. In addition, the study investigated whether glucose infusion, in the absence of exogenous insulin administration, would result in the development of clinical and histopathological evidence of laminitis. Glucose (50% dextrose) was infused intravenously at a rate of 0.68 mL/kg/h for 48 h in treated horses (n=4) and control horses (n=3) received a balanced electrolyte solution (0.68 mL/kg/h). Lamellar histology was examined at the conclusion of the experiment. Horses in the treatment group were insulin sensitive (M value 0.039±0.0012 mmol/kg/min and M-to-I ratio (100×) 0.014±0.002) as determined by an approximated hyperglycaemic clamp. Treated horses developed glycosuria, hyperglycaemia (10.7±0.78 mmol/L) and hyperinsulinaemia (208±26.1 μIU/mL), whereas control horses did not. None of the horses became lame as a consequence of the experiment but all of the treated horses developed histopathological evidence of laminitis in at least one foot. Combined with earlier studies, the results showed that laminitis may be induced by either insulin alone or a combination of insulin and glucose, but that it is unlikely to be due to a glucose overload mechanism. Based on the histopathological data, the potential threshold for insulin toxicity (i.e., laminitis) in horses may be at or below a serum concentration of ~200 μIU/mL. 相似文献
992.
de Laat MA Pollitt CC Walsh DM McGowan CM Sillence MN 《Veterinary journal (London, England : 1997)》2012,192(3):435-440
Persistent digital hyperthermia, presumably due to vasodilation, occurs during the developmental and acute stages of insulin-induced laminitis. The objectives of this study were to determine if persistent digital hyperthermia is the principal pathogenic mechanism responsible for the development of laminitis. The potent vasodilator, ATP-MgCl(2) was infused continuously into the distal phalanx of the left forefoot of six Standardbred racehorses for 48 h via intra-osseous infusion to promote persistent digital hyperthermia. The right forefoot was infused with saline solution and acted as an internal control. Clinical signs of lameness at the walk were not detected at 0 h, 24h or 48 h post-infusion. Mean ± SE hoof wall temperatures of the left forefoot (29.4 ± 0.25°C) were higher (P<0.05) than those on the right (27.5 ± 0.38°C). Serum insulin (15.0 ± 2.89 μIU/mL) and blood glucose (5.4 ± 0.22 mM) concentrations remained unchanged during the experiment. Histopathological evidence of laminitis was not detected in any horse. The results demonstrated that digital vasodilation up to 30°C for a period of 48 h does not trigger laminitis in the absence of hyperinsulinaemia. Thus, although digital hyperthermia may play a role in the pathogenesis of laminitis, it is not the sole mechanism involved. 相似文献
993.
Jean‐François HOCQUETTE Isabelle CASSAR‐MALEK Catherine JURIE Dominique BAUCHART Brigitte PICARD Gilles RENAND 《Animal Science Journal》2012,83(11):750-758
Genetic selection in favor of muscle growth at the expense of fat should affect characteristics of muscles, and therefore beef quality. This study was conducted with two extreme groups of six animals selected among 64 Charolais young bulls ranked according to their genetic potential for muscle growth. Muscle characteristics were assessed in Rectus abdominis (RA, slow oxidative) and Semitendinosus (ST, fast glycolytic) muscles. Intramuscular fat content and proportions of myosin heavy chains I (slow) and IIA (fast oxido‐glycolytic) and certain indicators of oxidative metabolism (activities of citrate synthase (CS), isocitrate dehydrogenase and cytochrome‐c oxidase (COX); expression of H‐fatty acid binding protein (FABP)) were higher in RA than in ST muscle. Genetic selection for muscle growth reduced intramuscular fat content and the activities of some oxidative metabolism indicators (namely CS, COX only). The positive correlation between muscle triacylglycerol content and A‐FABP messenger RNA level (a marker of adipocyte differentiation) (r = 0.53, P < 0.05) suggests that A‐FABP may be a good marker of the ability of bovines to deposit intramuscular fat. In conclusion, the metabolic muscle characteristics which respond to the selection process in favor of muscle growth clearly differ from the muscle characteristics which allow muscle types to be differentiated. 相似文献
994.
A systematic quantitative review of urban tree benefits,costs, and assessment methods across cities in different climatic zones 总被引:1,自引:0,他引:1
Urban trees can potentially mitigate environmental degradation accompanying rapid urbanisation via a range of tree benefits and services. But uncertainty exists about the extent of tree benefits and services because urban trees also impose costs (e.g. asthma) and may create hazards (e.g. windthrow). Few researchers have systematically assessed how urban tree benefits and costs vary across different cities, geographic scales and climates. This paper provides a quantitative review of 115 original urban tree studies, examining: (i) research locations, (ii) research methods, and (iii) assessment techniques for tree services and disservices. Researchers published findings in 33 journals from diverse disciplines including: forestry, land use planning, ecology, and economics. Research has been geographically concentrated (64% of studies were conducted in North America). Nearly all studies (91.3%) used quantitative research, and most studies (60%) employed natural science methods. Demonstrated tree benefits include: economic, social, health, visual and aesthetic benefits; identified ecosystem services include: carbon sequestration, air quality improvement, storm water attenuation, and energy conservation. Disservices include: maintenance costs, light attenuation, infrastructure damage and health problems, among others. Additional research is required to better inform public policy, including comparative assessment of tree services and disservices, and assessment of urban residents and land managers’ understanding of tree benefits and costs. 相似文献
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998.
Sabino CV Weese JS 《The Canadian veterinary journal. La revue veterinaire canadienne》2006,47(8):779-782
Bacterial contamination of multiple-dose saline bottles and medication vials in a veterinary teaching hospital was evaluated. Bacterial contamination was identified in 16/88 (18%) containers, with no difference in contamination between the large animal clinic, small animal clinic, and ruminant ambulatory clinic. Contamination of multiple-dose containers containing substances for injection was common, and potential pathogens were present in many situations. While the clinical significance is not resolved at this point, infection control practices should address this potential source of nosocomial infection. 相似文献
999.
Zintl A Mulcahy G de Waal T de Waele V Byrne C Clyne M Holden N Fanning S 《Irish veterinary journal》2006,59(9):495-500
Cryptosporidiosis, a protozoal disease which causes significant morbidity in humans, is one of the chief causes of diarrhoea in neonatal ruminants. although the parasite poses a significant threat to public health and animal health in Ireland, its epidemiology on the island is only poorly understood. Environmental studies have shown the waterborne parasite to be widespread in some untreated waterbodies around Ireland. The island's hydrogeological situation, combined with high stocking rates of livestock and the absence of filtration from regular water treatment, render it vulnerable to large-scale outbreaks. This review discusses the parasite in the Irish context and underlines the need for a reference facility to provide active surveillance on the island. 相似文献
1000.