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81.
Carter LJ 《Science (New York, N.Y.)》1980,207(4435):1057
82.
Carter LJ 《Science (New York, N.Y.)》1976,193(4259):1219-1222
83.
Carter LJ 《Science (New York, N.Y.)》1977,196(4294):1065
84.
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86.
Carter LJ 《Science (New York, N.Y.)》1974,186(4167):904
87.
88.
Carter LJ 《Science (New York, N.Y.)》1978,202(4373):1171
89.
Plagioclase and olivine crystals in the crystalline rocks from the Sea of Tranquillity show little or no evidence of either static or dynamic deformation. The large disorientations in many of the pyroxene crystals are commonly consistent with slip on the system T -(100), t = [001], but these distortions are not due to plastic flow. They are ascribed to rapid growth and quenching phenomena as deduced from studies of chondrules and of quenched natural and experimentally produced melts. Some of the silicates in the breccias and regolith show evidence of shock deformation, from mild to intense, as indicated by pervasive featuring, shock lamallae, and partial transformatiion of pyroxene and plagioclase crystals to glass. 相似文献
90.
Engineering enzyme specificity by "substrate-assisted catalysis" 总被引:8,自引:0,他引:8
A novel approach to engineering enzyme specificity is presented in which a catalytic group from an enzyme is first removed by site-directed mutagenesis causing inactivation. Activity is then partially restored by substrates containing the missing catalytic functional group. Replacement of the catalytic His with Ala in the Bacillus amyloliquefaciens subtilisin gene (the mutant is designated His64Ala) by site-directed mutagenesis reduces the catalytic efficiency (kcat/Km) by a factor of a million when assayed with N-succinyl-L-Phe-L-Ala-L-Ala-L-Phe-p-nitroanilide (sFAAF-pNA). Model building studies showed that a His side chain at the P2 position of a substrate bound at the active site of subtilisin could be virtually superimposed on the catalytic His side chain of this serine protease. Accordingly, the His64Ala mutant hydrolyzes a His P2 substrate (sFAHF-pNA) up to 400 times faster than a homologous Ala P2 or Gln P2 substrate (sFAAF-pNA or sFAQF-pNA) at pH 8.0. In contrast, the wild-type enzyme hydrolyzes these three substrates with similar catalytic efficiencies. Additional data from substrate-dependent pH profiles and hydrolysis of large polypeptides indicate that the His64Ala mutant enzyme can recover partially the function of the lost catalytic histidine from a His P2 side chain on the substrate. Such "substrate-assisted catalysis" provides a new basis for engineering enzymes with very narrow and potentially useful substrate specificities. These studies also suggest a possible functional intermediate in the evolution of the catalytic triad of serine proteases. 相似文献