Food safety in raw milk production: risk factors associated to bacterial DNA contamination

While human illness from milkborne pathogens may be linked to contamination of the product after pasteurization or improper pasteurization, such diseases are usually associated with consumption of raw milk or its by-products. Molecular biology tools were applied to investigate contamination by Listeria monocytogenes, Salmonella spp., some pathogenic strains of Escherichia coli, and Campylobacter jejuni in 548 raw milk samples from 125 dairy farms established in two regions from southern Brazil. Moreover, 15 variables were evaluated for their association with raw milk contamination levels, and the risk factors were determined by multiple regression analysis. Salmonella spp. were more frequently detected, followed by pathogenic E. coli. There was difference in contamination index between the regions, in which risk factors such as temporary cattle confinement, low milk production, low milking machine cleaning frequency, and milk storage area without tile walls were identified. The risk factors were specific to each region studied. Nevertheless, the data can be used to improve milk quality of dairy farms/herds with similar management practices.     

Nonsutured Hotz–Celsus technique performed by CO2 laser in two dogs and two cats

It is described a new version of modified Hotz‐Celsus technique performed in four patients, two cats and two dogs. The surgery was carried out using CO2 laser in order to avoid bleeding and decrease the anesthetic time. Besides, the wound created was not sutured and it healed by secondary intention without any complication. As a conclusion, this modification is a successful alternative to treat entropion in a safe and fast way.     

Monitoring of Brazilian wheat blast field populations reveals resistance to QoI,DMI, and SDHI fungicides

Wheat blast is one of the most important and devastating fungal diseases of wheat in South America, South-east Asia, and now in southern Africa. The disease can reduce grain yield by up to 70% and is best controlled using integrated disease management strategies. The difficulty in disease management is compounded by the lack of durable host resistance and the ineffectiveness of fungicide sprays. New succinate dehydrogenase inhibitor (SDHI) fungicides were recently introduced for the management of wheat diseases. Brazilian field populations of the wheat blast pathogen Pyricularia oryzae Triticum lineage (PoTl) sampled from different geographical regions in 2012 and 2018 were shown to be resistant to both QoI (strobilurin) and DMI (azole) fungicides. The main objective of the current study was to determine the SDHI baseline sensitivity in these populations. Moderate levels of SDHI resistance were detected in five out of the six field populations sampled in 2012 and in most of the strains isolated in 2018. No association was found between target site mutations in the sdhB, sdhC, and sdhD genes and the levels of SDHI resistance, indicating that a pre-existing resistance mechanism not associated with target site mutations is probably present in Brazilian wheat blast populations.     

Tectona grandis canopy cover predicted by remote sensing

The phytosanitary status of Tectona grandis plantations are monitored conventionally with periodic data collection in the field, which is often costly and has low efficiency. The objective of this research was to develop a methodology to predict the canopy cover of T. grandis plantations using multispectral images of the Sentinel-2 (S2) satellite and photographic imagery. The study was carried out in a T. grandis plantation of seminal origin, in Cáceres, Mato Grosso state, Brazil. Hemispherical photographic (HP) images of the plant canopy were obtained with a digital camera coupled to a “fisheye” lens fixed at 1.3 m high at two dates in the rainy and the dry season. Cloudless and no shadow images of the S2 satellite bands were concurrently obtained with the field images. Multivariate permutative analysis of variance (PERMANOVA) and partial least squares regression (PLSR) were used to predict canopy cover percentage. The accuracy of the predicted T. grandis canopy cover (%) by the PLSR model approach was 77.8?±?0.09%. The results indicate that a PLS model calibrated with 28 HP sample images can accurately estimate the percentage canopy cover for a continuous area of T. grandis plantations and facilitate mapping of canopy heterogeneity to monitor threats of diseases, mortality, fires, pests and other disturbances.

     

Overyielding in mixed pine forests with belowground complementarity: impacts on understory

European Journal of Forest Research - The impact of biodiversity loss on the functioning of forest ecosystems has become a central issue in ecology. Most reports of the positive effects of tree...     

Plant exposure to herbivore-induced plant volatiles: a sustainable approach through eliciting plant defenses

Journal of Pest Science - Modern agricultural policies across the globe are committed to a significant reduction in chemical pesticide dependency; however, pest management strategies are still...     

Use of a wireless system to measure invasive arterial blood pressure in ponies – preliminary study

Objective  To evaluate the feasibility and functionality of intra-carotid wireless device implantation in ponies, and to investigate its short-term complications.
Study design  Prospective preliminary study.
Animals  Five mixed breed, adult, intact male ponies weighing 104 ± 28.8 kg (mean ± SD) underwent surgery. Arterial blood pressure data were continuously collected from four animals.
Methods  General anesthesia was induced on two consecutive days. On the first day, an intra-arterial wireless device was implanted in the right carotid artery. On the next day, a transcutaneous intra-arterial catheter was placed in the left facial artery. Data from both sources were collected. Post-mortem examination was performed.
Results  Surgical time was 27.1 ± 11.85 minutes. All catheters remained in place with some extra vascular migration. Complications included mild seroma and hematoma.
Conclusion  The wireless system allowed continuous monitoring in ponies throughout anesthesia and at rest and may allow for the recording of arterial blood pressure and heart rate when it would be difficult to achieve with a conventional system (e.g. during recovery from anesthesia).
Clinical relevance  The wireless invasive blood pressure monitor may allow continuous measurements when only intermittent measurements would be feasible with a wired system.