Artifactual changes in canine blood following storage, detected using the ADVIA 120 hematology analyzer

BACKGROUND: Artifactual changes in blood may occur as a consequence of delayed analysis and may complicate interpretation of CBC data. OBJECTIVE: The aim of this study was to characterize artifactual changes in canine blood, due to storage, using the ADVIA 120 hematology analyzer. METHODS: Blood samples were collected into EDTA from 5 clinically healthy dogs. Within 1 hour after blood sample collection and at 12, 24, 36 and 48 hours after storage of the samples at either 4 degrees C or room temperature (approximately 24 degrees C), a CBC was done using the ADVIA 120 and multispecies software. A linear mixed model was used to statistically evaluate significant differences in values over time, compared with initial values. RESULTS: The HCT and MCV were increased significantly after 12 hours of collection at both 4 degrees C and 24 degrees C, and continued to increase through 48 hours. The MCHC initially decreased significantly at 12-24 hours and then continued to decrease through 48 hours at both temperatures. Changes in HCT, MCV, and MCHC were greater at 24 degrees C than at 4 degrees C at all time points. A significant increase in MPV and a decrease in mean platelet component concentration were observed at all time points at 24 degrees C. Samples stored at 24 degrees C for 48 hours had significantly higher percentages of normocytic-hypochromic RBCs, and macrocytic-normochromic RBCs, and lower platelet and total WBC counts. CONCLUSIONS: Delayed analysis of canine blood samples produces artifactual changes in CBC results, mainly in RBC morphology and platelet parameters, that are readily detected using the ADVIA 120. Refrigeration of specimens, even after 24 hours of storage at room temperature, is recommended to improve the accuracy of CBC results for canine blood samples.     

Soil organic matter dynamics and losses in manured maize-based forage systems

Soil organic matter (SOM) produces positive effects on multiple soil properties. Increasing its level also provides an opportunity to reduce atmospheric concentration of carbon dioxide (CO₂). Recycling animal manure and returning crop residue are among the main practices to enhance organic carbon (C) stock in arable croplands. This study analysed data of soil organic carbon (SOC) stocks from a medium-term field trial (established in 1992) through a Hénin–Dupuis-based equation to determine the proportion of different organic materials retained in the soil as SOM.The treatments included in the experiment simulated different typical management techniques and implied the application to the soil of various organic materials, i.e. cattle slurry, cattle farmyard manure, maize straw, root, maize and ley stubble. Soil organic carbon and total nitrogen (N) (Kjeldhal N) were measured in the 0–30 cm layer during spring 1999 and spring 2003. The relationship between SOC and N stocks measured in 1999 and 2003 and the annual additions of C and N was described by the Hénin–Dupuis-based equation, assuming the presence of two C and N pools (stable and fresh).Our results showed that the application of farmyard manure, slurry and maize straw induced higher C and N content compared to the application of urea without return of crop residue. The different levels of urea application did not produce any significant difference in C and N soil content.We found that approximately 2% of SOC is lost to the atmosphere annually. Furthermore, the amount of C and N retained in the soil each year varied by organic materials: 46% C and 44% N with farmyard manure, 26% C and 11% N with slurry, 28% C and 10% N with root and 6% C with maize straw and ley stubble.These results were used to inform an estimate of C lost or removed to/from atmosphere following manure application and crop residue return for the compilation of greenhouse gas inventories. We found that the most C conservative management is the production and spreading of farmyard manure and that increasing amounts of mineral N fertilizer did not affect the C sequestration extent.     

Molecular mechanism regulating axoneme activation in marine fish: a review

River Gomti, a tributary of river Ganga in northern India, is being polluted due to indiscriminate disposal of domestic sewage and industrial wastes that contain genotoxic chemicals. The study was conducted to evaluate the genotoxic potential of polluted water of river Gomti in two fish species, namely Channa punctatus and Mystus vittatus. The fishes were exposed in situ in nylon cages to the polluted water of river Gomti fixed near a distillery outlet located in Lucknow. The induction of DNA damage and micronuclei were determined in blood erythrocytes using comet assay and micronucleus test, respectively. The induction in micronuclei frequencies and DNA damage were found to be significantly elevated (p?<?0.01) in exposed specimens after 3?days post-exposure as compared to the control, i.e. from laboratory-acclimatized fish specimens. The comparison of DNA damage between the two species indicated that C. punctatus is more sensitive to aquatic pollutants. Thus, this fish could be used as a bio-indicator of genotoxicity for bio-monitoring of water bodies. The results further revealed that the river Gomti is being contaminated with potential genotoxic and mutagenic chemicals produced from industrial and domestic activities; therefore, immediate measures are needed to reduce the inflow of pollutants in the river.     

Assessment of Dormancy and Sprouting Behavior of CIP Elite and Advanced Clones Under Different Storage Conditions in Uzbekistan

In potato breeding and selection, storability should be regarded as equally important as yield, disease resistance, and quality. A study documenting the dormancy period, sprouting behavior, and weight loss of 17 International Potato Center potato elite and advanced clones was carried out in Tashkent, Uzbekistan, under cellar and cold store conditions, during 2008 and 2009. Ninety tubers of each of 17 clones were allocated to experimental units of 30 tubers each placed in trays and randomized in three replications following a random complete block design. Therefore, there were three replications of 30 seed tubers each per entry. The dormancy period ranged from 77 to 115 days and from 100 to 186 days under cellar and cold storage, respectively. There was a relatively high positive correlation (0.69) for dormancy period between storage systems, indicating that clones demonstrating longer and shorter dormancy periods under one system will also behave similarly under the other system. A negative correlation (−0.53 and −0.88) was found between dormancy period and length of the longest sprout in cellar and cold store, respectively, meaning that clones with shorter dormancy often showed a greater length of their longest sprout. The weight loss percentage per tuber was similar in both storage systems, from 5.0% to 8.0% in the cellar and from 5.0% to 9.8% in the cold store, although for different storage periods (an average of 110 and 166 days under cellar and cold storage conditions, respectively). The study indicated that under cellar conditions, clones with a longer dormancy period and slower rate of sprout growth have less weight loss during storage and therefore better keeping quality.     

早期检测以有效控制火鸡抱窝

尽管已取得了良好的遗传进展,并拥有较好的种群管理技术,但母火鸡的抱窝行为仍时常发生。特别是在炎热气候条件下,母火鸡的抱窝现象更甚。目前,预防仍是减少抱窝行为发生的关键。这不仅包括早期检测,而且还包括使母火鸡尽快恢复产蛋的一些实用措施。     

Innovative light management to improve production sustainability,overall quality,and the phenolics composition of nectarine (Prunus persica cv. Stark Red Gold)

Summary

This research was conducted under the framework of the ISAFRUIT Project and aimed to investigate the effects of different light micro-environments on the final overall quality of nectarine fruit production. Experiments were conducted in a commercial orchard of the nectarine (Prunus persica) ‘Stark Red Gold’ during 2006 and 2007. Reflective mulches were laid down in the inter-row spaces in mid-May. Those environmental conditions affected by mulching such as temperature and reflected light were monitored until fruit harvest. Fruit production per tree was enhanced by mulch-treatment in both years, but the differences were statistically significant only in 2006, when the average fruit weight was also enhanced. Nectarines became more ripe in the 2006 season, whereas no differences in the main fruit quality indices were detected in 2007.The concentrations of phenolic compounds in ripe nectarines were positively enhanced in both years. The increase in concentration of overall phenolic compounds (in mg 100 g^?1 fresh weight) was calculated to be approx. 60% in 2006 and 2007, indicating an interesting improvement of the nutraceutical and anti-oxidant potential of nectarines. Experiments were also conducted using UV plus white light irradiation under controlled conditions. The accumulation of phenolic compounds, specifically anthocyanin concentrations, in nectarines previously screened using paper bags were determined at different times after irradiation. The results clearly indicated an inducing effect of UV plus white light irradiation on the synthesis and accumulation of anthocyanins in fruit skin. The consequences for the colour and health potential of nectarines are discussed.