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41.
The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating‐induced endometritis 下载免费PDF全文
CE Fedorka KE Scoggin EM Woodward EL Squires BA Ball MHT Troedsson 《Reproduction in domestic animals》2017,52(1):89-96
In the horse, breeding induces a transient endometrial inflammation. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating‐induced endometritis PMIE Select seminal plasma proteins cysteine‐rich secretory protein‐3 (CRISP‐3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro. The objective of this study was to determine whether the addition of CRISP‐3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Six mares classified as susceptible to PMIE were inseminated during four consecutive oestrous cycles with treatments in randomized order of: 1 mg/ml CRISP‐3, 150 μg/ml lactoferrin, seminal plasma (positive control) or lactated Ringer's solution (LRS; negative control) to a total volume of 10 ml combined with 1 × 109 spermatozoa pooled from two stallions. Six hours after treatment, an endometrial biopsy was obtained for qPCR analysis of selected genes associated with inflammation (pro‐inflammatory cytokines interleukin (IL)‐1β, IL‐8, tumour necrosis factor (TNF)‐α, interferon (INF)‐γ, anti‐inflammatory cytokines IL‐1RN and IL‐10, and inflammatory‐modulating cytokine IL‐6). Seminal plasma treatment increased the mRNA expression of IL‐1β (p = .019) and IL‐8 (p = .0068), while suppressing the mRNA expression of TNF (p = .0013). Lactoferrin also suppressed the mRNA expression of TNF (p = .0013). In conclusion, exogenous lactoferrin may be considered as one modulator of the complex series of events resulting in the poorly regulated pro‐inflammatory response seen in susceptible mares. 相似文献
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CE Short 《Veterinary anaesthesia and analgesia》2005,32(4):16-17
There was only limited emphasis on anesthesia and pain management in veterinary colleges in Russia until the last ten years. There is now great interest in improvement by Russia practitioners. This effort is hampered by the lack of approved and available medications and limited instructions. At this time, the small animal practitioners have tranquilizers, alpha2 agonists local anesthetics and NSAID's for their use. Propofol is available through pharmacies and efforts are being made for veterinarians to use ketamine. Only a few practices have inhalant anesthetic units and halothane. During a recent lecture tour of Russia, general clinical principles and specific anesthetic and pain management protocols were taught. The audience, primarily young and progressive veterinarians, actively participated and showed great desire to practice quality anesthesia and pain management for their patients. Teaching young veterinarians how to manage their cases without the availability of opioids, ketamine advanced anesthetic equipment and monitors was indeed challenging. Protocols for perioperative management consisting of medetomidine with or without local anesthesia followed by carprofen for post-operative pain is an improvement. We sometimes spend so much time discussing which of many drug combinations we will use but seldom consider what we would do if we were practicing our specialty in parts of the world where we would have to modify dosages and combinations to achieve humane care with few drug choices. 相似文献
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A 5-month-old female Rottweiler dog was diagnosed to have a neurodegenerative disease that has been recently report ed in Rottweilers from North America and Europe. The dog was presented with progressive signs of ataxia, tetraparesis and inspiratory stridor. The clinical investigation included analysis of CSF, radiography, myelography and electrophysiolog-ical testing. No evidence of vertebral malformation or inflammatory CNS disease was identified. Bilateral laryngeal paraly sis was identified in the lightly anaesthetised dog. Electromyography showed abnormal spontaneous activity from the intrin sic musculature of the larynx. At necropsy there were no gross abnormalities of the nervous system but there was atrophy of the dorsal cricoarytenoid muscles of the larynx. There were widespread histological abnormalities throughout the ner vous system including neuronal vacuolation, spongiform changes in the neuropil and axonal degeneration which was most prominent in the spinal cord. These clinical and pathological findings are consistent with the diagnosis of a new neurode-generative disease reported from North America and Europe. This diagnosis is of particular significance in Australia where transmissible spongiform encephalopathies have not been identified. 相似文献
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DAVID BRICKMAN LORNA TAYLOR ÁSTA GUDMUNDSDÓTTIR GUDRUN MARTEINSDÓTTIR 《Fisheries Oceanography》2007,16(5):448-458
A characteristic of biophysical models (BPM) is that they contain a number of parameters that are poorly known or only known within a range of possible values. This paper describes an efficient optimized BPM developed to simulate the Icelandic cod pelagic 0‐group survey data. The method is based on presenting the results as a probability density function (PDF) that a particle released from a given spawning drifts downstream to a given grid location some time later. The model determines egg production model parameters (peak spawning time, spawning duration, number of eggs released) for each of 15 spawning grounds as the solution of a bound constrained optimization problem that minimizes model‐data misfits in abundance and age distributions. The model is applied to simulating the 2002 and 2003 summer survey data. The model does a reasonable job of simulating the observed inshore / offshore abundance gradient and spatial age gradient for each year. Problem areas are explained from the point of view of model limitations. We caution that the results from an optimized model should always be assessed with the model’s limitations in mind and with respect to whatever biological data are available. 相似文献
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T. E. MAITHO P. LEES J. B. TAYLOR † 《Journal of veterinary pharmacology and therapeutics》1986,9(1):26-39
The disposition of phenylbutazone (4.4 mg/kg), administered intravenously to six Welsh Mountain ponies, was described by a two-compartment open model. Pharmacokinetic parameters were not significantly different after morning dosing in comparison with afternoon dosing. When phenylbutazone (4.4 mg/kg) was administered orally to the same ponies, marked variations in time to peak concentrations were produced with different feeding schedules. When access to hay was permitted before and after dosing, the mean time to peak concentration was 13.2 +/- 1.2 h and double peaks in the plasma concentration-time curve were common. Double peaks were also encountered when phenylbutazone was given to ponies deprived of food prior to, and allowed access to hay after, dosing. In this circumstance, mean times to peak concentration were much shorter (3.8 +/- 1.3 h after morning dosing and 5.3 +/- 1.5 h followed afternoon dosing). Absorption was more regular and double peaks were less apparent when food was withheld both before and after dosing. In order to explain these findings, it is tentatively postulated that, whereas some of the administered dose of phenylbutazone may be absorbed quickly, some may become adsorbed on to the feed and subsequently released by fermentative digestion in the large intestine and/or caecum. The consequences of delayed absorption in fed animals for toxicity and clinical efficacy, and for the use of phenylbutazone in equestrian sports, are considered. Delayed absorption in ponies given access to hay was not accompanied by a significant reduction in total absorption. Bioavailability was estimated to be approximately 69% in fed and 78% in unfed ponies. Estimates of bioavailability gave similar values for morning (72%) and afternoon (71%) dosing. 相似文献
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Serological detection and identification of bacteria from plants by the conjugated Staphylococcus aureus slide agglutination test 总被引:3,自引:1,他引:2
A rapid slide agglutination test using polyclonal antisera conjugated to protein A-rich whole-cell Staphylococcus aureus was developed for the detection and identification of bacteria from plants. The specificity and sensitivity of the technique was evaluated in 18 antibody/antigen combinations, representing six bacterial genera ( Erwinia, Lactobacillus, Pseudomonas, Rhizobium, Rhodococcus and Xanthomonas ). For two pathovars of Pseudomonas syringae the specificity of the technique was increased by the use of antisera prepared to somatic extracts.
The advantages of the Staphylococcus aureus agglutination technique include speed, simplicity and the ability to identify organisms directly from infected plant tissues. It was applied to the detection of Pseudomonas syringae pv. phaseolicola and pv. pisi in lesions on bean and pea, respectively, to P. gladioli pv. alliicola and Lactobacillus sp. from rotted onion bulbs and specific strains of Rhizobium phaseoli in bean root nodules. 相似文献
The advantages of the Staphylococcus aureus agglutination technique include speed, simplicity and the ability to identify organisms directly from infected plant tissues. It was applied to the detection of Pseudomonas syringae pv. phaseolicola and pv. pisi in lesions on bean and pea, respectively, to P. gladioli pv. alliicola and Lactobacillus sp. from rotted onion bulbs and specific strains of Rhizobium phaseoli in bean root nodules. 相似文献
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