《种子专业综合实验》课程教学的改革与探索

为进一步提高种子专业人才的培养质量,满足现代种业对应用型人才的需求,对《种子专业综合实验》这门专业课程,从课程内容体系的构建、实验教学形式改革、实验考核方式改革等方面进行了探讨,进而全面提高本课程的教学水平和教学质量。     

奶牛子宫内膜组织中MUC-1的表达及与炎症的关系

为了探讨黏蛋白-1(MUC-1)的表达与奶牛子宫内膜炎的关系,本试验在屠宰场采集奶牛子宫样本12个,通过形态学和制作组织切片观察病理变化,将子宫样本分成健康组和炎症组,分别提取各组子宫组织中的总RNA,扩增MUC-1基因,采用凝胶电泳和灰度扫描的方法,计算MUC-1的相对表达量。结果发现,子宫内膜炎患牛子宫组织中MUC-1 mRNA的表达量显著高于健康组(P<0.05)。可以推测,MUC-1参与了奶牛子宫内膜组织的炎症过程。     

海狸色獭兔生长发育及生长曲线的拟合

本文采用Logistic非线性动物生长模型拟合海狸色獭兔从出生到6月龄的平均体重,进行生长曲线拟合分析,结果表明:①利用Logistic生长曲线方程,确定獭兔生长曲线方程各参数为a=23.08,b=1.39,k=2 718.65,拟合后,建立海狸色獭兔生长曲线方程为y=2718.65/(1+23.08 e-1.39x)(P<0.05);②Logistic方程能够很好地拟合海狸色獭兔生长过程,拟合度达到0.98,并计算出生长拐点时间为2.81月龄,拐点体重为1 359.33g。     

持续高温应激对肥育猪生产性能、胴体性状、背最长肌营养物质含量及肌纤维特性的影响

本试验旨在研究持续30℃的环境高温应激对肥育猪生产性能、胴体性状、背最长肌质构分析、营养物质含量及肌纤维特性的影响.试验选取8窝共24头体重为(79.0±1.5) kg的“杜长大”三元阉公猪,其中每3头来自同一窝的猪只随机分到3组,每组8个重复,每个重复1头,3组分别为对照组(22℃适温+自由采食)、高温应激组(30℃高温+自由采食)和采食量配对组(22℃适温+采食量配对),试验期21d.结果表明:1)持续高温应激显著降低肥育猪平均日增重(ADG) (P＜0.05),极显著降低肥育猪平均日采食量(ADFI) (P＜0.01).2)3组之间的肥育猪胴体重、胴体长、平均背膘厚和皮厚均差异不显著(P＞0.05),高温应激组肥育猪背最长肌大理石花纹评分在宰后24 h有低于对照组的趋势(P＜0.10).3)高温应激组肥育猪背最长肌的剪切力极显著高于对照组和采食量配对组(P＜0.01).与采食量配对组相比,高温应激组肥育猪背最长肌胶黏性和硬度有降低的趋势(P＜0.10).4)高温应激组和采食量配对组肥育猪背最长肌粗蛋白质含量显著高于对照组(P＜0.05),且2组的背最长肌肌内脂肪(IMF)含量极显著低于对照组(P＜0.01).5)高温应激组肥育猪背最长肌Ⅰ型肌纤维含量有低于采食量配对组的趋势(P＜0.10),采食量配对组的Ⅱx型肌纤维含量显著低于对照组(P＜0.05).由此可见,肥育后期高温应激会改变猪表观肉品质和肌肉营养物质含量.     

抗裂与易裂枣内源激素含量和细胞壁代谢相关酶活性比较

 以枣抗裂果品种‘圆铃枣’和易裂果品种‘俊枣’为材料,测定了果实生长发育曲线、果 形指数及种子败育率,并对果皮、果肉、种子中内源激素含量及果皮、果肉中细胞壁代谢相关酶的活性 进行了检测。结果表明,‘俊枣’果形指数和种子败育率均显著高于‘圆铃枣’。果实发育后期‘俊枣’ 果皮中的GA3 含量、果肉中的IAA 含量明显高于‘圆铃枣’,而‘圆铃枣’果肉及种子中的ABA 含量高 于‘俊枣’;‘俊枣’果肉及种子中（GA3 + IAA + ZT）/ABA 的比值在整个果实生长发育期均高于‘圆铃 枣’。果实生长发育后期‘俊枣’果皮中的果胶酶及纤维素酶活性高于‘圆铃枣’,且‘俊枣’果肉中的 POD 及PPO 活性也较高。以上结果显示,枣果实生长发育后期易裂品种‘俊枣’果肉中的IAA 积累较多, 而抗裂品种‘圆铃枣’果肉及种子中ABA 含量显著高于易裂品种;易裂品种‘俊枣’果肉及种子中（GA3 + IAA + ZT）/ABA 的比值较高,果皮中的果胶酶、纤维素酶活性影响裂果的发生,其POD 及PPO 活性相 对较高。     

Effect of over-expression of transcription factor CDX2 on proliferation and cell cycle of human gastric cancer cell line SGC-7901

AIM: To study the effect and the molecular mechanism of CDX2 over-expression on the proliferation, growth and cell cycle of human gastric cancer cell line SGC-7901. METHODS: The SGC-7901 cells in LV-CDX2-GFP group were transfected with the recombinant lentivirus vector LV-CDX2-GFP, the cells in LV-GFP group were transfected with the negative control lentiviral vector for the negative control, and the cells in blank control group were without any treatment. The cell proliferation was detected by CCK-8 assay. The cell cycle distribution was analyzed by flow cytometry. The expression of CDX2, Bax, Bcl-2, cyclin D1 and survivin was determined by semi-quantitative RT-PCR and Wes-tern blotting. RESULTS: Compared with LV-GFP group and blank control group, the proliferation activity of the SGC-7901 cells was significantly lower (P<0.05), the G0/G1 phase proportion increased (P<0.05), the mRNA and protein levels of Bcl-2, cyclin D1 and survivin were reduced (P<0.05), and the mRNA and protein levels of Bax were up-regulated (P<0.05) in LV-CDX2-GFP group. No statistically significant difference of the above indexes was observed (P>0.05) between LV-GFP group and blank control group. CONCLUSION: Over-expression of CDX2 mediated by lentivirus inhibits the proliferation and growth of human gastric cancer SGC-7901 cells and arrestes the cell cycle at G0/G1 phase, which may be related to down-regulation of Bcl-2, cyclin D1 and survivin and up-regulation of Bax.     

功能性堆肥及其浸提液在设施温室甜瓜栽培上的应用研究

以甜瓜蜜世界为试材,采用单因素完全随机设计,研究了功能性堆肥及其浸提液对甜瓜生长指标、叶绿素荧光参数、品质、单果质量及产量的影响,探讨堆肥及其浸提液在设施温室甜瓜栽培上的应用效果．为其在植物生产上的应用提供依据。结果表明,堆肥及其浸提液促进根系发育,有利于甜瓜叶面积、茎粗和株高的增加及叶片叶绿素的累积;显著提高qP,对Fv／Fm影响不显著;促进果实营养物质的累积,提高产量且改善品质;使甜瓜提前上市5-6d;降低施肥量63．28％以上,进而减少肥料成本42．86％。     

温性荒漠草原不同放牧强度草地锰和铜动态变化

为探讨不同放牧强度下温性荒漠草原土-草体系中微量元素锰和铜的变化,对土壤、牧草中锰和铜含量进行了测定。结果表明,除中牧区以外,其他放牧区土壤锰含量随季节的推移极显著下降(P0.01),夏季牧区极显著高于其他放牧区,冬春季和秋季则轻牧区极显著高于其他放牧区(P0.01)。除对照外,轻牧,中牧和重牧区土壤铜含量夏季极显著高于其他两季(P0.01),冬春季和秋季不放牧或轻牧区极显著高于中牧和重牧区(P0.01)。各放牧区牧草中锰和铜(除中牧区)含量均冬春季显著或极显著高于夏秋两季,同一季节不同放牧区则各有变化。     

亲吻素-1基因对雄性哺乳动物生殖调控的研究进展简

亲吻素-1（Kiss-1）基因参与哺乳动物性腺轴的调节,同时也参与其他生殖功能的调控,作者综述了Kiss-1基因在雄性哺乳动物生殖系统的研究进展,并对该领域今后的研究方向进行了分析。     

Seed traits and taxonomic relationships determine the occurrence of mutualisms versus seed predation in a tropical forest rodent and seed dispersal system

Although many studies have been carried out on plant–animal mutualistic assemblages, the roles of functional traits and taxonomy in determining both whether interactions involve mutualisms or predation and the structure of such assemblages are unclear. We used semi‐natural enclosures to quantitatively assess the interaction strengths between seeds of 8 sympatric tree species and 4 rodent species in a tropical forest in Xishuangbanna, Yunnan, Southwest China. We found 2 clusters of species in the seed–rodent network represented by 2 genera in the Fagaceae (Castanopsis, Lithocarpus). Compared to seeds of 3 Castanopsis species, seeds with heavy weight, hard coat or caloric content (including 3 Lithocarpus species) were eaten less and more frequently hoarded by rodents. In turn, hoarded seeds showed less predation and more mutualism with rodents. Our results suggest that seed traits significantly affected the hoarding behavior of rodents, and, consequently, the occurrence of mutualisms and predation as well as assemblage structure in the plant–animal seed dispersal system. Taxonomically‐related species with similar seed traits as functional groups belong to the same substructures in the assemblage. Our results indicate that both seed traits and taxonomic relationships may simplify thinking about seed dispersal systems by helping to elucidate whether interactions are likely to be dominated by predation or mutualism.