Total substitution of fish oil by vegetable oils in Senegalese sole (Solea senegalensis) diets: effects on fish performance, biochemical composition, and expression of some glucocorticoid receptor-related genes

To study the substitution of fish oil by vegetable oils in fish diets, juveniles Senegalese sole (Solea senegalensis) were fed diets (56 % crude protein, 12 % crude lipid) containing either linseed (100LO) or soybean (100SO) oils in comparison with a 100 % fish oil-based diet (100FO) for 90 days. Samples of muscle, liver, and intestine were collected for biochemical analysis and for glucocorticoid receptor-related genes, including GR1 and GR2, and the associated heat shock proteins HSP70, HSP90AA, and HSP90AB. Besides, basal levels of plasma cortisol were also determined. After the feeding period, a stress test, consisting on 5 min of net chasing, was applied to a selected population of each dietary group. Total replacement of fish oil by vegetable oils did not induced changes in fish growth and performance, but affected fatty acid profile of muscle, liver, and intestine, reflecting those tissues the characteristic fatty acids of each type of dietary oil. A tendency to conserve the ARA/EPA ratio could be observed in the different tissues, despite of the level of these fatty acids in diet. Chasing stress induced an increase of muscle GR1 and a reduction in intestinal GR2 relative expressions at any of the experimental diets assayed. In liver, chasing stress induced an increase in both GR1 and GR2 gene expression in fish fed fish oil diets. Similarly, chasing stress induced an increase of muscle HSP70 and decrease of HSP90AB in liver at any of the experimental diet assayed. Besides, vegetable oils decreased the expression of HSP70 in intestine, being the relative expression of liver HSP90AA increased by the inclusion of linseed oil in the diet, at any of the experimental conditions assayed.     

Choosing and rejecting cattle and sheep: changing discourses and practices of (de)selection in pedigree livestock breeding

This paper examines the discourses and practices of pedigree livestock breeding, focusing on beef cattle and sheep in the UK, concentrating on an under-examined aspect of this—the deselection and rejection of some animals from future breeding populations. In the context of exploring how animals are valued and represented in different ways in relation to particular agricultural knowledge-practices, it focuses on deselecting particular animals from breeding populations, drawing attention to shifts in such knowledge-practices related to the emergence of “genetic” techniques in livestock breeding which are arguably displacing “traditional” visual and experiential knowledge’s of livestock animals. The paper situates this discussion in the analytical framework provided by Foucault’s conception of “biopower,” exploring how interventions in livestock populations aimed at the fostering of domestic animal life are necessarily also associated with the imperative that certain animals must die and not contribute to the future reproduction of their breed. The “geneticization” of livestock breeding produces new articulations of this process associated with different understandings of animal life and the possibilities of different modes of intervention in livestock populations. Genetic techniques increasingly quantify and rationalize processes of selection and deselection, and affect how animals are perceived and valued both as groups and as individuals. The paper concludes by emphasizing that the valuation of livestock animals is contested, and that the entanglement of “traditional” and “genetic” modes of valuation means that there are multiple layers of valuation and (de)selection involved in breeding knowledge-practices.     

利用ACGM分子标记研究10个毛竹不同栽培变种的遗传多样性

应用40对ACGM引物扩增10个毛竹不同栽培变种及其2个近缘种材料,结果有35对引物可以在至少1个材料中得到特异性PCR产物,其中27对引物在12个供试材料中表现出多态性,占引物总数的67.5%.8对引物在供试材料间没有多态性位点,进一步对无多态性位点的扩增序列研究表明,相同引物扩增出的序列间差异性很小.从拷贝数来看,在水稻中表现为单拷贝的基因,有些在竹子中表现为多拷贝的特性;有些在部分供试材料中为单拷贝,而在另一些供试材料中表现为多拷贝.聚类结果表明:在10个毛竹不同栽培变种中,绿槽毛竹和黄槽毛竹的亲缘关系最近;圣音毛竹与其他不同栽培变种的毛竹亲缘关系最远.     

抗白粉病小麦新种质的选育及细胞学鉴定

利用杂交、回交、自交相结合的方法从小麦烟农15与中问偃麦草（Thinopyrum intermedium（Host）Bark worth and Dewey,ElE1E2E2StSt,2n=6x=42）杂交后代（BC3F6）中选出抗白粉病种质系SN 996221。在对其白粉病抗性等主要农艺性状鉴定的基础上,进行了细胞学分析。结果表明：SN996221高抗小麦白粉病,其抗性可能来源于中问偃麦草;其根尖细胞染色体数目为2n=42,花粉母细胞减数分裂中期I（PMC MI）染色体构型为2n=21Ⅱ,与普通小麦的杂种F1PMC M I大多数细胞能观察到2个单价体,染色体构型为2n=1.02I＋20.49Ⅱ,SN 996221可能是一个小麦一中间偃麦草的异代换系。     

不同杀虫剂对稻纵卷叶螟和稻飞虱的防治效果

选取含有不同有效成分的12种杀虫剂,在第四（2）代稻纵卷叶螟二三龄幼虫盛期和稻飞虱的卵孵盛期进行了田间用药试验。结果表明：氟虫腈和含有氟虫腈成分的复配药剂,对稻纵卷叶螟和稻飞虱均表现出良好的速效性和持效性,控害效果也相对较高。毒死蜱与氟虫腈或与阿维菌素复配,可达到优势互补,即保持良好速效性的同时,其持效性可得到明显提高;在稳定防治效果的基础上,各药剂有效成分的使用量可明显降低。     

Change in soil properties and the soil microbial community following land spreading of olive mill wastewater affects olive trees key physiological parameters and the abundance of arbuscular mycorrhizal fungi

Olive mill wastewater (OMW) constitutes a major environmental problem for Mediterranean countries, where most of the world olive oil production takes place. The recycling of the OMW and its use as water for irrigation in agriculture, provided that its impact on soil and plant is established, is an attractive possibility for the Mediterranean countries. Investigations were performed on the influence of agronomic application of OMW (amount applied: 30, 60, 100 and 150 m³ ha^?1) in a field of olive trees on trees characters (photosynthesis, root-soluble carbohydrate and root colonisation), soil properties, and soil microbial community structure. Specific attention was paid to arbuscular mycorrhizal (AM) fungi. The soil fatty acid methyl ester (FAME) 16:1ω5 was used to quantify biomass of AM fungi and the root FAME 16:1ω5 analysis was used as index for the development of colonisation in the olive trees roots. A significant increase in organic C, C/N ratio, extractable phosphorus and exchangeable potassium was found after one year of agronomic application of OMW. The development of saprophytic fungi was significantly higher in the OMW amended soils, whereas the abundance of the soil FAME 16:1ω5, root FAME 16:1ω5, photosynthetic rates and the amount of the total root-soluble carbohydrate were decreased significantly after agronomic application of OMW. A principal component analysis (PCA) of the trees characteristics profiles showed discrimination between the nonirrigated and the OMW irrigated olive trees. These findings suggest that the altering functioning of arbuscular mycorrhizas should be considered as potential factors mediating olive trees responses to agronomic application of OMW when the OMW dose applied is higher than 30 m³ ha^?1. To our knowledge, this is the first report of alterations in the soil FAME 16:1ω5 and root FAME 16:1ω5 due to land spreading of OMW.     

Reduced tillage effects on physical properties of silt loam soils growing root crops

In the scope of the increasing concern for soil conservation, reduced tillage (RT) agriculture is growing more important in today's agriculture in Western Europe. However, crop rotations often include beets and potatoes, crops that are generally assumed to be less suitable under RT agriculture because they result in a high disturbance of the soil at the formation of the ridges and at harvest. Therefore, the short- and long-term effect of RT agriculture on bulk density (BD), water retention curve (WRC), aggregate stability and field-saturated hydraulic conductivity of silt loam soils with crop rotations including root crops was evaluated. Ten fields at seven locations representing the important RT types, applied for a different number of years, and eight fields under conventional tillage (CT) agriculture with similar soil type and crop rotation were selected. At each location, BD of the 5–10 cm layer was mostly lower in the RT fields (1.42 ± 0.05 Mg m⁻³ [average with standard deviation]) compared to the CT fields (1.44 ± 0.09 Mg m⁻³) and the water content at saturation was mostly higher (0.394 ± 0.027 m³ m⁻³ and 0.382 ± 0.021 m³ m⁻³ for RT and CT fields, respectively). No differences in BD (1.53 ± 0.03 Mg m⁻³) or WRC could be found in the 25–30 cm soil layer when comparing the RT with the CT fields. The stability index of the 0–10 cm layer measured by ‘dry and wet sieving’ [De Leenheer, L., De Boodt, M., 1959. Determination of aggregate stability by the change in mean weight diameter. Mededelingen van landbouwhogeschool en de opzoekingstations van de staat te Gent 24, 290–300] was 40% higher under RT than CT agriculture. The mean weight diameter (MWD) [Le Bissonnais, Y., 1996. Aggregate stability and assessment of soil crustability and erodibility: I. Theory and methodology. Eur. J. Soil Sci. 47, 425–437] was significantly higher even after short-term RT compared to CT agriculture. The MWD after a heavy shower, a slow wetting of the soil and stirring the soil after prewetting was 19%, 38% and 34% higher for RT than CT fields, respectively. The field-saturated hydraulic conductivity tended to be higher under RT compared to the CT fields. Despite the high disturbance of the soil every 2 or 3 years of crop rotations including sugar beets or potatoes, RT agriculture had a positive effect on the investigated physical soil properties.     

Salt stress effects on enzymes activities involved in carbon metabolism and nitrogen availability of two Tunisian durum wheat varieties

Two varieties of durum wheat (Om Rabiaa and Karim), were analyzed and evaluated in the presence of increasing doses of NaCl (0, 100, 200 and 300?mM) in which we added different concentrations of nitrate (0.1, 3, 10?mM). The data obtained showed that presence of NaCl in the culture medium induces the increase of the salt accumulation levels (Na⁺, Cl^?) and reduces the levels of K⁺ and NO₃^? in the cultivar Om Rabiaa. In Karim variety, ions that have been heavily accumulated following exposure to NaCl are Na⁺ and K⁺ while low levels of NO₃^? and Cl^? have been detected. Those findings highlight the difference in the salinity tolerance of durum wheat cultivars also depending on nitrogen (N) availability, Karim cultivar being less sensitive to NaCl treatment than Om Rabiaa. These data also suggested a relationship between salt tolerance capacity and enhancement of nitrogen and carbon metabolisms enzyme activity.     

Changes in phytochemical, antimicrobial and free radical scavenging activities of the Peruvian pepper tree (Schinus molle L.) as influenced by fruit maturation

The effect of stage of maturity on total lipids, fatty acids, yields and essential oil composition and their antimicrobial and free radical scavenging activities of the Schinus molle fruits was investigated. The content of total lipids varied from 2.87 to 5.35% (w/w, dw) and were rich in unsaturated fatty acids particularly linoleic acid. As maturation progress, the essential oil yield dropped from 5.18% to 1.15%. Monoterpene hydrocarbons with α-phellandrene (35.15-40.38%), limonene + β-phellandrene (21.47-36.62%), β-myrcene (7.61-24.96%) and α-pinene (1.92-2.58%) were found to be the main components. At the same time, the essential oils were evaluated for their antimicrobial and free radical scavenging activities. They were found to be active against Bacillus subtilis, Bacillus cereus, Salmonella typhymurium and Escherichia coli but they do not inhibit the growth of Candida albicans. Conversely, they showed very weak activity against the DPPH radical. In both assay, the oil derived from the intermediate stage was reported as more efficient.     

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