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41.
Temporal bone preservation in a 2600-year-old Egyptian mummy 总被引:1,自引:0,他引:1
Perforated eardrums in the preserved temporal bones of a mummified Egyptian male in otherwise normal middle ears offer new evidence of acute otitis media and probable defective hearing among ancient Egyptians. 相似文献
42.
Wu CC Li TK Farh L Lin LY Lin TS Yu YJ Yen TJ Chiang CW Chan NL 《Science (New York, N.Y.)》2011,333(6041):459-462
Type II topoisomerases (TOP2s) resolve the topological problems of DNA by transiently cleaving both strands of a DNA duplex to form a cleavage complex through which another DNA segment can be transported. Several widely prescribed anticancer drugs increase the population of TOP2 cleavage complex, which leads to TOP2-mediated chromosome DNA breakage and death of cancer cells. We present the crystal structure of a large fragment of human TOP2β complexed to DNA and to the anticancer drug etoposide to reveal structural details of drug-induced stabilization of a cleavage complex. The interplay between the protein, the DNA, and the drug explains the structure-activity relations of etoposide derivatives and the molecular basis of drug-resistant mutations. The analysis of protein-drug interactions provides information applicable for developing an isoform-specific TOP2-targeting strategy. 相似文献
43.
Tandem regions of yeast DNA topoisomerase II share homology with different subunits of bacterial gyrase 总被引:16,自引:0,他引:16
The nucleotide sequence for the Saccharomyces cerevisiae gene TOP2, which encodes DNA topoisomerase II, was compared with the sequence for bacterial DNA gyrase. The amino and carboxyl terminal halves of the single-subunit yeast enzyme showed homologies with the B and A subunits of bacterial gyrase, respectively, at corresponding positions along the polypeptide chains. Although the two enzymes differ in both quaternary structure and activity, the homology between the two proteins indicates mechanistic as well as structural similarities, and a probable evolutionary relationship. 相似文献
44.
Frankland PW Bontempi B Talton LE Kaczmarek L Silva AJ 《Science (New York, N.Y.)》2004,304(5672):881-883
Although the molecular, cellular, and systems mechanisms required for initial memory processing have been intensively investigated, those underlying permanent memory storage remain elusive. We present neuroanatomical, pharmacological, and genetic results demonstrating that the anterior cingulate cortex plays a critical role in remote memory for contextual fear conditioning. Imaging of activity-dependent genes shows that the anterior cingulate is activated by remote memory and that this activation is impaired by a null alpha-CaMKII mutation that blocks remote memory. Accordingly, reversible inactivation of this structure in normal mice disrupts remote memory without affecting recent memory. 相似文献
45.
46.
Atlas R Campbell P Cozzarelli NR Curfman G Enquist L Fink G Flanagin A Fletcher J George E Hammes G Heyman D Inglesby T Kaplan S Kennedy D Krug J Levinson R Marcus E Metzger H Morse SS O'Brien A Onderdonk A Poste G Renault B Rich R Rosengard A Salzburg S Scanlan M Shenk T Tabor H Varmus H Wimmer E Yamamoto K;Journal Editors Authors Group 《Science (New York, N.Y.)》2003,299(5610):1149
47.
Surface- and volume-limited chemical reactions on and in atmospheric aerosol particles cause growth while changing organic composition by 13 to 24% per day. Many of these particles contain carbonaceous components from mineral dust and combustion emissions in Africa, Asia, and North America and reveal reaction rates that are three times slower than those typically used in climate models. These slower rates for converting from volatile or hydrophobic to condensed and hygroscopic organic compounds increase carbonaceous particle burdens in climate models by 70%, producing organic aerosol climate forcings of as much as -0.8 watt per square meter cooling and +0.3 watt per square meter warming. 相似文献
48.
Anderson WA Amasino RM Ares M Banerjee U Bartel B Corces VG Drennan CL Elgin SC Epstein IR Fanning E Guillette LJ Handelsman J Hatfull GF Hoy RR Kelley D Leinwand LA Losick R Lu Y Lynn DG Neuhauser C O'Dowd DK Olivera T Pevzner P Richards-Kortum RR Rine J Sah RL Strobel SA Walker GC Walt DR Warner IM Wessler S Willard HF Zare RN 《Science (New York, N.Y.)》2011,334(6057):760-761
49.
Lynn H. Booth Vanessa J. Heppelthwaite Kathryn O’Halloran 《Journal of Soils and Sediments》2002,2(3):148-154
Objectives and Methods. The sensitivity of two biomarkers, the neutral red retention assay (NRRA) and cytochrome P450 were evaluated in the earthwormAporrectodea caliginosa for their potential to detect exposure to benzo[a]pyrene (BaP). Cytochrome P450 was also evaluated in the earthwormLumbricus rubellus, and measured using the substrate ethoxycoumarin. Optimal assay conditions (pH, and temperature) were determined, followed
by exposure of earthworms to 20 mg/kg BaP (a typical concentration at contaminated sites in New Zealand). Ethoxycoumarin-O-dealkylase
(ECOD) activity was measured after 7, 14, and 21 days exposure. The NRRA was evaluated in earthworms exposed to 0.2, 20, and
100 mg BaP/kg, and biomarker responses were compared with effects on body weight.
Results and Discussion Benzo[a]pyrene failed to induce ECOD activity in either earthworm species, and therefore it is not useful as a biomarker
of BaP exposure and was not evaluated further. In all cases, the NRRA was significantly affected in the absence of any effects
on earthworm body weight, indicating that this assay can detect exposure to BaP at a range of concentrations comparable to
those found at contaminated sites. The NRRA should be linked to reproductive endpoints, then it can be used as an early warning
indicator of adverse effects. Establishing biomarker stability under environmental conditions is an important step in biomarker
development. Therefore, the effects of soil type (sandy soil, silt loam, and a clay soil), moisture content (15–30%), and
temperature (5–20°) on the NRRA were determined. In all cases, there was no effect on the NRRA, indicating that this assay
is very stable under varying environmental conditions.
Conclusion and Outlook In conclusion, cytochrome P450 activity does not appear to be a useful indicator of PAH exposure in eitherA. caliginosa or L.rubellus, and due to the inherently low activity, it is not suitable as a routine biomarker for detecting environmental contamination
by these compounds. In contrast, the NRRA in the earthworm A.caliginosa is a promising indicator of PAH exposure at the concentrations likely to be found in contaminated sites in New Zealand, and
therefore has potential for evaluating these contaminated sites. If the NRRA can be linked to ecologically important life-cycle
endpoints, such as reproduction, then it could be used as an early warning indicator of adverse effects at contaminated sites,
i.e., by measuring biomarker responses in earthworms from a ‘contaminated area’ and comparing these with earthworms from a
matched control area. 相似文献
50.
Turf-banked terrace treads and risers, turf exfoliation and possible relationships with advancing treeline 总被引:4,自引:0,他引:4
Fine-scale geomorphic/pedogenic processes at alpine treeline may facilitate the initial stages of conifer invasion of alpine tundra. Turf-banked terraces and turf exfoliation associated with solifluction may provide both the topographic protection and the seedbed necessary for conifer establishment above treeline. The morphometry, stoniness and surface penetrability of turf-banked terraces were recorded. Whereas differences among sites exist due to topographic constraints, differences in soil penetrability depend on turf exfoliation. Exfoliated turf risers are significantly more penetrable than non-exfoliated solifluction risers and adjacent treads. These penetrable microsites provide favorable conditions for seed germination. The process of turf exfoliation may play a role in conifer advance into tundra during climatic change. 相似文献