Regulation of oceanic silicon and carbon preservation by temperature control on bacteria

We demonstrated in laboratory experiments that temperature control of marine bacteria action on diatoms strongly influences the coupling of biogenic silica and organic carbon preservation. Low temperature intensified the selective regeneration of organic matter by marine bacteria as the silicon:carbon preservation ratio gradually increased from approximately 1 at 33 degrees C to approximately 6 at -1.8 degrees C. Temperature control of bacteria-mediated selective preservation of silicon versus carbon should help to interpret and model the variable coupling of silicon and carbon sinking fluxes and the spatial patterns of opal accumulation in oceanic systems with different temperature regimes.     

Changes in peroxidase and phenols activity in apple fruit inoculated with antagonistic Pseudomonas fluorescens isolates and Botrytis mali

The biocontrol activity of three isolates of Pseudomonas fluorescens against gray mold of apple fruit caused by Botrytis mali and their ability to induce biochemical defense response in apple tissue were investigated. Apple fruit (Malus domestica) wounds were inoculated with 20 microL bacterial suspension (10(8) CFU mL(-1)) of Pseudomonas fluorescens followed 24 h later by 20 microL of conidial suspension of B. mali (10(5) conidia mL(-1)). The apples were then incubated at 20 degrees C for 11 days. Lesion diameters were evaluated 6 and 10 days after pathogen inoculation. In addition to controlling gray mold, these three isolates of P. fluorescens caused increase in peroxidase activities that reached maximum levels 2-6 days after pathogen inoculation. Phenolic accumulation was increased in apple fruit treated with antagonists and inoculated with B. mali and exhibited the highest level 6-8 days after treatment. The ability of P. fluorescens to increase activities of peroxidase and levels of phenol compounds maybe one of mechanism responsible its biocontrol activity.     

Application of the CSM-CERES-Rice model for evaluation of plant density and irrigation management of transplanted rice for an irrigated semiarid environment

The objectives of this study were to evaluate the performance of the Cropping System Model (CSM)-CERES (Crop-Environment Resource Synthesis)-Rice for simulating growth and yield of rice under irrigated conditions for a semiarid environment in Pakistan and to determine the impact of plant density and irrigation regime on grain yield and economic returns. The crop simulation model was evaluated with experimental data collected in 2000 and 2001 in Faisalabad, Punjab, Pakistan. The experiment utilized a randomized complete block design with three replications and included three plant densities (one seedling hill^?1, PD₁; two seedlings hill^?1, PD₂; and three seedlings hill^?1, PD₃) and five irrigation regimes (625 mm, I₁; 775 mm, I₂; 925 mm, I₃; 1075 mm, I₄; and 1225 mm, I₅). To determine the most appropriate combination of plant densities and irrigation regimes, four plant densities from one seedling hill^?1 to four seedlings hill^?1 and 17 irrigation regimes ranging from 0 to 1600 mm, for a total of 68 different scenarios, were simulated for 35 years of historical daily weather data. The evaluation of CSM-CERES-Rice showed that the model was able to accurately simulate growth and yield of rice for irrigated semiarid conditions, with an average error of 11% between simulated and observed grain yield. The results of the biophysical analysis showed that the combination of the two seedlings hill^?1 plant density and the 1,300 mm irrigation regime produced the highest yield compared to all other scenarios. Furthermore, the economic analysis through the Mean-Gini Dominance (MGD) also showed the superiority of this treatment compared to the other treatment combinations. The mean monetary return ranged from ?47 to 1,265 $ ha^?1 among all 68 scenarios. However, to be able to furnish the demand of rice grain for local consumption and to increase export, there is a need to expand this technology among the rice growers of other rice producing areas in Pakistan through extension workers.