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81.
Matsudomi N Kanda Y Yoshika Y Moriwaki H 《Journal of agricultural and food chemistry》2004,52(15):4882-4886
The effects of alphas-casein on heat aggregation of ovotransferrin (OT) were studied by heating at 80 degrees C for 20 min in 10 mM phosphate buffer, pH 7.0. The heat interactions between alphas-casein and OT were followed by turbidity development and polyacrylamide gel electrophoresis. We found that alphas-casein can effectively suppress the heat-induced aggregation of heat-labile OT. The suppressive ability of alphas-casein was reduced by the presence of NaCl on heating. Dephosphorylated alphas-casein had less ability to suppress the aggregation of OT than native alphas-casein. Our results indicate that alphas-casein interacts with the heat-denatured OT through its exposed hydrophobic surface and phosphoserine residue. Such interactions seem to be important in helping to suppress the aggregation of heated OT. The suppressive effects of alphas-casein on heat aggregation of OT would be partially ascribed to the formation of transparent gel from egg white by the addition of alphas-casein. 相似文献
82.
Low temperature-induced sterility in rice: Evidence for the effects of temperature before panicle initiation 总被引:4,自引:0,他引:4
Spikelet sterility of rice (Oryza sativa L.) results from low temperatures during panicle development. However, this temperature alone cannot fully explain the fluctuations in sterility observed in the field, since the susceptibility of rice plants to low temperature often changes according to its physiological status during sensitive stages. In the present study, we examined whether temperatures before the panicle initiation stage (PI) influence the plant's susceptibility to sterility during panicle development. To test this, we conducted a 2-year pot study and an analysis of field data using a model of cooling degree-days (CDD). In the pot study, the air temperature (Ta) and water temperature (Tw) were controlled independently for 3 weeks during the vegetative growth stage before PI. After PI, the plants were submerged in a cool water bath at a critical temperature of 19.5 °C to induce floral sterility. We found that low Tw during vegetative growth significantly increased the sterility. Low Ta during vegetative growth also significantly increased the sterility, but this effect was diminished by warm Tw even at low Ta. There was a close and negative correlation between sterility and Tw during vegetative growth. In the analysis of field data, we introduced CDD using temperatures below a threshold level of 20 °C to represent the magnitude of the exposure to low temperature from PI to the heading stage. Data of Ta was used for this analysis because data of Tw was scare. The CDD model was applied to 77 independent data sets collected at nine Agricultural Research Centers during four typical cool summers (1980, 1988, 1993, and 2003) in northern Japan. Year-to-year variations in sterility at one site were roughly accounted for by the variations in CDD, but large deviations were observed among the years. The deviations were related to Ta averaged over the 30-day period before PI. For a similar level of CDD, the lower the Ta before PI, the greater the sterility. Similar deviations were observed in the between-site relationships between sterility and CDD, and these deviations were related with the Ta before PI. These results suggest that temperatures before PI, and especially Tw, change the susceptibility of a rice plant to low temperatures during panicle development. 相似文献
83.