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31.
为建立适宜玉米SSR—PCR的反应体系和扩增程序,利用正交设计L16(4^5)表,对反应体系的模板DNA、dNTPs、Primers、Taq DNA聚合酶的浓度进行4因素4水平优化筛选和扩增程序的优化,确立了最优反应体系和扩增程序。即在10μL体系中,模板DNA20ng,1×PCR buffer,dNTPs62.5pmol/μL,Primers0.25pmol/μL,Taq DNA polymerase0.5U。反应程序为:94℃预变性5min;94℃变性45S,60℃退火45S,72℃延伸1min,32个循环;72℃延伸5min,4℃保存。使用300对SSR引物对重组近交系的两亲本扩增,筛选出条带清晰,有差异的引物94对,用于基因连锁图谱构建和QTL定位。 相似文献
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研究了不同培养基、激素和外植体对刺五加愈伤组织诱导的影响,结果表明:在旺盛生长期获取的叶片可以在附加植物激素的BZ培养基上以较高频率诱导出愈伤组织,诱导率为41.58%。刺五加组织培养的适宜外植体为叶片和当年生根,适宜基本培养基为BZ(改良培养基),且适合刺五加愈伤组织诱导和继代培养的激素组合为ZT0.2+NAA2.0+GA31.5。 相似文献
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Rogers CS Stoltz DA Meyerholz DK Ostedgaard LS Rokhlina T Taft PJ Rogan MP Pezzulo AA Karp PH Itani OA Kabel AC Wohlford-Lenane CL Davis GJ Hanfland RA Smith TL Samuel M Wax D Murphy CN Rieke A Whitworth K Uc A Starner TD Brogden KA Shilyansky J McCray PB Zabner J Prather RS Welsh MJ 《Science (New York, N.Y.)》2008,321(5897):1837-1841
Almost two decades after CFTR was identified as the gene responsible for cystic fibrosis (CF), we still lack answers to many questions about the pathogenesis of the disease, and it remains incurable. Mice with a disrupted CFTR gene have greatly facilitated CF studies, but the mutant mice do not develop the characteristic manifestations of human CF, including abnormalities of the pancreas, lung, intestine, liver, and other organs. Because pigs share many anatomical and physiological features with humans, we generated pigs with a targeted disruption of both CFTR alleles. Newborn pigs lacking CFTR exhibited defective chloride transport and developed meconium ileus, exocrine pancreatic destruction, and focal biliary cirrhosis, replicating abnormalities seen in newborn humans with CF. The pig model may provide opportunities to address persistent questions about CF pathogenesis and accelerate discovery of strategies for prevention and treatment. 相似文献
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Zoonotic diseases of cats 总被引:1,自引:0,他引:1
J R August A S Loar 《Veterinary Clinics of North America: Small Animal Practice》1984,14(5):1117-1151
As veterinarians, our responsibilities do not end with the care of our patients. The welfare of our clients and their families depends on our ability to detect and control potentially zoonotic diseases in their pets. Because some of these zoonoses can have devastating effects on the development of the unborn fetus or on family health in general, discussion about these diseases between veterinarian and client is often emotionally charged. Under such circumstances, the offering of inaccurate and erroneous information by the veterinarian can have drastic consequences. It is likely that other zoonotic diseases of domestic pets will be identified in the future, especially those that can cause opportunistic infections in debilitated and immunodeficient persons. In the meantime, the potential hazards of cat ownership can be significantly reduced through an increased understanding of feline diseases and an improved level of health care for our feline patients. 相似文献
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J R August 《Veterinary Clinics of North America: Small Animal Practice》1983,13(3):585-597
Several areas of feline gastroenterology deserve critical attention in the near future. For example, as compared with the dog, little is known about the various causes of malabsorptive disease in the cat. So frequently, intestinal biopsy samples reveal nothing more than intestinal thickening with fibrosis and nonspecific mild cellular infiltration, and the inciting cause is never determined. It is, perhaps, wrong to be critical about the use of the bark of Berberis vulgaris and the root of Rheum in modern feline gastroenterology, since most of us occasionally use unconventional therapies. It has been rumored that I have been known to advocate the daily addition of a tablespoon of pumpkin-pie filling to the food of cats suffering from recurrent constipation and acquired megacolon. The rumors are true, and colonic evacuation is sometimes promoted with the use of this unusual bulking agent after traditional drug therapy has failed. We all have our weaknesses! 相似文献
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