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21.
We evaluated support for four alternate hypotheses explaining the distribution of breeding Brown-headed Cowbirds (Molothrus ater) in forests at varying distances from the forest edge in three Midwestern USA landscapes with varying amounts of forest fragmentation
(core forest area ranged from 5 to 70%). We focused on breeding cowbirds’ use of forest because of the risk of nest parasitism
to forest-dwelling hosts and to identify factors affecting breeding cowbird habitat selection. We compared distances of cowbird
locations in the forest from the forest edge (“edge distances”) to distances of random forest locations in the entire landscape
or within individual cowbird home ranges. We analyzed 1322 locations of 84 cowbirds across three landscapes. We found support
for the landscape context hypothesis that breeding cowbird preference for forest edge varied with landscape context. Ninety
percent of cowbird locations were within 150–350 m of forest edge, despite the overall availability of forest at greater distances
from edge (as far as 500–1450 m) both within cowbird home ranges and the entire forested landscape. Cowbird preference for
edge varied by landscape context largely due to differences in the availability of forest edge. In a highly fragmented forest
cowbirds utilized the entire forest and likely viewed it as “all edge.” In less fragmented forests, cowbirds preferred edge.
We consider how variation in cowbird edge preference might relate to patterns in host abundance, host diversity, and host
quality because cowbird movements indicate they are capable of using forest farther from edges. 相似文献
22.
23.
Puckhaber LS Dowd MK Stipanovic RD Howell CR 《Journal of agricultural and food chemistry》2002,50(24):7017-7021
The dimeric sesquiterpene gossypol occurs naturally in cottonseed and other parts of the cotton plant. Gossypol exists as enantiomers because of the restricted rotation around the central binaphthyl bond. The (-)-enantiomer is toxic to nonruminant animals while the (+)-enantiomer exhibits little, if any, toxicity to these animals. Developing cotton plants with low levels of the (-)-gossypol could expand the use of cottonseed as a feed source. Gossypol also may play a role in protecting the plant from pathogens. The relative toxicity of (+)- and (-)-gossypol to plant pathogens has not been reported. We measured the concentration of (+)- and (-)-gossypol in roots from cotton seedlings that were treated with the biocontrol agent Trichoderma virens that induces biosynthesis of gossypol and related terpenoids in cotton roots. (-)-Gossypol was the minor enantiomer in control and treated roots, but levels were slightly higher in roots from T. virens-treated seed. We also determined the toxicity of the gossypol enantiomers and the racemate to the seedling disease pathogen Rhizoctonia solani. The (+)- and (-)-enantiomers of gossypol and the racemate are equally effective in inhibiting growth of this pathogen. The lethal doses of the gossypols required to kill the pathogen appeared to be similar, but their toxicities are significantly less than those of related cotton and kenaf sesquiterpenes. The results indicate that altering the enantiomeric ratio in cotton roots will not adversely affect the resistance of seedlings to the seedling pathogen R. solani. 相似文献
24.
25.
Functional role of aspartic acid-27 in dihydrofolate reductase revealed by mutagenesis 总被引:12,自引:0,他引:12
E E Howell J E Villafranca M S Warren S J Oatley J Kraut 《Science (New York, N.Y.)》1986,231(4742):1123-1128
The crystal structures and enzymic properties of two mutant dihydrofolate reductases (Escherichia coli) were studied in order to clarify the functional role of an invariant carboxylic acid (aspartic acid at position 27) at the substrate binding site. One mutation, constructed by oligonucleotide-directed mutagenesis, replaces Asp27 with asparagine; the other is a primary-site revertant to Ser27. The only structural perturbations involve two internally bound water molecules. Both mutants have low but readily measurable activity, which increases rapidly with decreasing pH. The mutant enzymes were also characterized with respect to relative folate: dihydrofolate activities and kinetic deuterium isotope effects. It is concluded that Asp27 participates in protonation of the substrate but not in electrostatic stabilization of a positively charged, protonated transition state. 相似文献
26.
Turnover of ribosomal RNA in rat liver 总被引:14,自引:0,他引:14
After a single injection of radioactive orotic acid and a "chase" of nonradioactive precursor, the specific activity of ribosomal RNA in rat liver decreases logarithmically at a rate corresponding to a half-life of about S days. The possible significance of this result is discussed with regard to control of protein synthesis. 相似文献
27.
Spatial characterization of acidification related parameters in sensitive regions of Atlantic Canada
S. R. Esterby A. H. El-Shaarawi G. D. Howell T. A. Clair 《Water, air, and soil pollution》1989,46(1-4):289-303
Many lakes in eastern Canada are sensitive to long range transport of atmospheric pollutants because of their low buffering capacity. Thus, it is important to assess long term changes in water quality. Due to the large number of lakes, a method was needed for choosing a subset of lakes to monitor regularly. Preliminary surveys were conducted in Nova Scotia and Newfoundland in which water quality parameters connected with acidification were measured in a set of lakes in each region. This paper describes the division of each set of lakes into several groups, with lakes in a group having similar water quality parameter values, by means of cluster analysis and principal component ordination. The characteristics of the groups are shown by graphical procedures and summary statistics, and this characterization is used to both determine the number of groups and describe the final choice. The membership of the groups was subsequently assessed in terms of the influences of terrestrial weathering, marine aerosols, anthropogenically derived mineral acids and natural organic acids. 相似文献
28.
29.
Witch flounder Glyptocephalus cynoglossus has recently been identified as a candidate species for aquaculture in the northeastern United States and the Canadian Atlantic Provinces. This study investigated the optimal temperatures for witch flounder larval first feeding and for long term larval culture from hatching through metamorphosis. Maximum first feeding occurred between 15.0 and 16.2 C. Larvae did not survive beyond first feeding when reared at mean temperatures of 5.1, 10.4, or 19.5 C and were unable to initiate feeding at mean rearing temperatures below 6.0 C. At a rearing temperature of 15.0 C in 16-L tanks, mean larval survival to 60 days post hatch (dph) was 14.1%. Mean overall length-specific growth rate for larvae reared to 60 dph at 15.0 C was 3.5%/d and mean absolute growth was 0.62 mm/d. Subsequent larval growth at 15.6 C began to taper off towards 70 dph at the onset of weaning which overlapped with larval metamorphosis. Growth plateaued at 85 dph, followed by a rebound between 90 and 95 dph. Survival was 100% when weaning onto a dry, pelleted diet was initiated at 70 dph with a 10-d live diet co-feeding period. These results are favorable and encourage the further pursuit of commercial witch flounder culture. 相似文献
30.
James Howell 《Pest management science》1982,13(4):367-372
Samples taken from a ryegrass variety trial at the Grassland Research Institute, Hurley, and examined at the Welsh Plant Breeding Station, showed that the dominant stem borer species in the overwintering generation were Geomyza tripunctata Fall. and Oscinella frit L. The rate of parasitisation of stem borers, largely by species of Hymenoptera, ranged from 15 to 43%. There were differences in host plant selection by Geomyza compared with Oscinella spp. The numbers of both stem borer groups were significantly associated with the total number of tillers per sample; however, this relationship was found to differ markedly between varieties. 相似文献