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91.
Summary Common blight disease in beans (Phaseolus vulgaris), caused by the bacterium Xanthomonas campestris pv. phaseoli, reduces crop yield and seed quality. Information is needed on the variation of leaves and pods disease reaction to strains of the bacterium after different inoculation methods. Phaseolus vulgaris cultivars Red Kidney Charlevoix, GN Harris, GN 1140, and GN Emerson were inoculated with three different strains of Xanthomonas campestris pv. phaseoli at two inoculum concentrations (108 and 106 bacterial cells/ml) using water soaking, multiple needle, and razor blade inoculation on leaves, and razor blade scratch, dissecting needle, and razor blade cut inoculation on pods. Differential cultivar disease reactions of leaves, pods, or both to the bacterial strains were observed in some cases. Significant interactions among cultivars, inoculation methods, strains, and inoculum concentrations (leaves) were found. A rapid leaf chlorosis developed 6 to 7 days after inoculation. Strains of bacteria did not show specificity in inducing this reaction, but rapid leaf chlorosis was associated with high inoculum concentration and with the water soaking and multiple needle methods. Another experiment was conducted to count the number of living bacterial cells deposited in the leaf tissue after inoculation by different methods. The number of bacteria deposited by water soaking or multiple needle was higher than that deposited by razor blade.Published as Paper No. 8584, Journal Series, Nebraska Agricultural Experiment Station. Research was conducted under Project No. 20–36. 相似文献
92.
Anne Elings 《Euphytica》1993,70(1-2):85-96
Syrian durum wheat landraces from diverse collection sites were evaluated for agronomic performance under arid conditions over two seasons at four locations, at two levels of nutrient availability.Grain yield differed considerably among locations. Within locations, significant population and fertilizer effects were demonstrated. In most cases, maximum yield was achieved by landraces, which demonstrates the breeding value of local germplasm. In a particular environment, the population effect was nonsignificant for total dry matter production, but significant for straw and grain yield. Nitrogen application was ineffective if moisture availability was the dominant growth limiting factor.Correlations between plant characteristics at evaluation and collection site characteristics were limited, and therefore, their utilization in germplasm evaluation appear limited. The negative relation between grain yield and soil nitrogen content at the collection site may be caused by the comparative disadvantage that germplasm from regions with favourable growing conditions has under low-yielding conditions.Particular environmental conditions at the collection site were no indicator for the response to changed growing conditions. Relatively high grain yields under good and adverse growing conditions excluded each other in all but two cases. Also, it was difficult to relate grain yield to regions of collection, as the response of individual landraces from a certain region to changing growing conditions differed strongly. Differentiation on the basis of landrace groups appears more promising. The Hauran landrace group provided the largest number of populations with relatively high grain yields over diverse environments. 相似文献
93.
Summary S1 to S5 inbred lines, derived from a maize population bred for its overall resistance to three tropical viruses, were screened for resistance to maize streak virus (MSV) by artificial plant infection using viruliferous leafhoppers. Symptoms were rated and intra-line frequency distributions studied for all pedigree inbred lines. Mortality due to MSV was very low among these inbreds. Symptoms appeared later, developed slower and were less severe than in the susceptible control hybrid. Results of a study of 500 S1 and 93 S2 lines suggested that resistance is under genetic control via a system involving loci with major genes (with dominance for resistance) controlling high to complete resistance, associated with a genetic system involving loci with minor genes controlling partial resistance. Lines expressing complete resistance to MSV were developed from 5 cycles of inbreeding and selection. The relevance of such complete and partial resistance is discussed.Abbreviations MRPS
Mean Rating for Plants exhibiting Symptoms 相似文献
94.
This study was designed to examine whether or not specific tree species (Picea glauca, Picea mariana, Pinus banksiana, Populus tremuloides), their post-fire stand age, or their position in a successional pathway had any significant effect on the functional diversity of associated soil microbial communities in a typical mixed boreal forest ecosystem (Duck Mountain Provincial Forest, Manitoba, Canada). Multivariate analyses designed to identify significant biotic and/or abiotic variables associated with patterns of organic substrate utilization (assessed using the BIOLOG™ System) revealed the overall similarity in substrate utilization by the soil microbial communities. The five clusters identified differed mainly by their substrate-utilization value rather than by specific substrate utilization. Variability in community functional diversity was not strongly associated to tree species or post-fire stand age; however, redundancy analysis indicated a stronger association between substrate utilization and successional pathway and soil pH. For example, microbial communities associated with the relatively high pH soils of the P. tremuloides-P. glauca successional pathway, exhibited a greater degree of substrate utilization than those associated with the P. banksiana-P. mariana successional pathway and more acidic soils. Differences in functional diversity specific to tree species were not observed and this may have reflected the mixed nature of the forest stands and of their heterogeneous forest floor. In a densely treed, mixed boreal forest ecosystem, great overlap in tree and understory species occur making it difficult to assign a definitive microbial community to any particular tree species. The presence of P. tremuloides in all stand types and post fire stand ages has probably contributed to the large amount of overlap in utilization profiles among soil samples. 相似文献
95.
Sebastian Klimek Anne Richter gen. Kemmermann Johannes Isselstein 《Biological conservation》2007,134(4):559-570
Decline of grassland diversity throughout Europe within the last decades is threatening biological diversity and is a major conservation problem. There is an urgent need to determine the underlying factors that control vascular plant species richness and composition in managed grasslands. In this study, 117 grasslands were sampled using standardised methods. Explanatory variables were recorded for each grassland site, reflecting the local field management, site-specific environmental conditions and large-scale spatial trends. Using variation partitioning methods, we determined the pure and shared effects of these three sets of explanatory variables on the plant species richness and composition in grasslands. Most of the explained variation in plant species richness was related to the joint effect of local field management and environmental variables. However, the applied variation partitioning approach revealed that the pure effect of spatial variables contributed relatively little to explaining variation in both the plant species richness and species composition. The largest fractions of explained variation in plant species composition were accounted for by the pure effects of environmental and local field management variables. Moreover, the results revealed that the main mechanisms by which these sets of explanatory variables affect plant species vary according to the type of management regime under study. From our findings we could conclude that particularly a reduction of nitrogen fertilisation on meadows and grazing at a low stocking rate on pastures can help to conserve biodiversity. 相似文献
96.
Jose Maria da Silva Paulo Cezar Rezende Fontes Carla do Carmo Milagres July Anne Amaral de Abreu 《Journal of plant nutrition》2020,43(18):2833-2843
Abstract Adequate plant nutrition is important to reduce costs and increase the crop yield. This study tried to verify the influence of Nitrogen (N) on plant biomass production and the yield of bell pepper grown in SLAB; quantify the N use efficiency (NUE), and to quantify the residual N concentration in the substrate after the end of the crop cycle. The experiment was carried out in a protected environment. Pepper seedlings were transplanted to SLAB bags containing 40?dm3 of substrate. The treatments consisted of six N rates (0, 1.5, 3.0, 4.5, 6.0, and 7.5?g plant?1) split into 10 fortnightly applications, in a randomized block design with four replications. The fruit yield was evaluated throughout the experiment and after the final harvest, the dry mass of leaves, stem, and root of the plant, the N content and accumulated, the residual nitrate and ammonium in the substrate, and the NUE were evaluated. The rate of 5.51?g plant?1 of N, corresponding to 355.5?kg ha?1, provided the maximum yield of commercial fruits of 1.57?kg plant?1 or 101.2 t ha?1. With increasing N rate, the residual nitrate concentration in the substrate increased and the NUE decreased. 相似文献
97.
No-take reserves constitute one tool to improve conservation of marine ecosystems, yet criteria for their placement, size, and arrangement remain uncertain. Representation of biodiversity is necessary in reserve planning, but will ultimately fail for conservation unless factors affecting species’ persistence are also incorporated. This study presents an empirical example of the divergent relationships among multiple metrics used to quantify a site’s conservation value, including those that address representation (habitat type, species richness, species diversity), and others that address ecological processes and viability (density and reproductive capacity of a keystone species, in this case, the black chiton, Katharina tunicata). We characterized 10 rocky intertidal sites across two habitats in Barkley Sound, British Columbia, Canada, according to these site metrics. High-richness and high-production sites for K. tunicata were present in both habitat types, but high richness and high-production sites did not overlap. Across sites, species richness ranged from 29 to 46, and adult K. tunicata varied from 6 to 22 individuals m−2. Adult density was negatively correlated with species richness, a pattern that likely occurs due to post-recruitment growth and survival because no correlation was evident with non-reproductive juveniles. Sites with high adult density also contributed disproportionately greater potential reproductive output (PRO), defined by total gonad mass. PRO varied by a factor of five across sites and was also negatively correlated with species richness. Compromise or relative weighting would be necessary to select valuable sites for conservation because of inherent contradictions among some reserve selection criteria. We suspect that this inconsistency among site metrics will occur more generally in other ecosystems and emphasize the importance of population viability of strongly interacting species. 相似文献
98.
BACKGROUND: Positive secular trends in adolescent obesity and an increased prevalence of fear of fatness, particularly among girls, have been documented world-wide. There is a lack of consensus about assessment criteria for childhood obesity and no standard exists for assessing Irish children. In 1990, the Irish National Nutrition Survey used body mass index (BMI) > or =26 kg m(-2) to describe the prevalence of overweight among Irish adolescents. OBJECTIVES: (1) To examine the range in classification of Dublin schoolchildren as overweight according to four standard assessment methods; (2) to assess changes in weight status, prevalence of fear of fatness and accompanying slimming practices in a one-year follow-up; and (3) to compare the prevalence of overweight with that documented in 1990 among adolescents of similar age. DESIGN: A one-year follow-up study of 199 healthy schoolchildren (90 boys and 109 girls; mean age of 11 years at baseline) attending seven fee-paying (six single- and one mixed-sex) and eight non-fee-paying (four single- and four mixed-sex) primary schools in Dublin city centre. MEASUREMENTS: Weight, height, waist circumference and triceps skinfold were measured and used in five definitions of overweight, including published cut-off points of BMI-for-age (Centers for Disease Control and Prevention BMI-for-age charts for boys and girls; BMI reference curves for the UK 1990; International Obesity Task Force age- and sex-specific BMI cut-offs), actual relative weight and BM > or =26 kg m(-2). Assessment of body image perceptions and satisfaction (using figure line drawings) was reported in a questionnaire specifically designed for this study. RESULTS: The prevalence of overweight within the total group differed between the four standard definitions of weight status, by 9% at baseline and 8% at follow-up. Accordingly, increasing trends over the year ranged from zero to 3%. Using the criterion BMI > or =26 kg m(-2), 6% of Dublin schoolchildren were overweight, compared with 1.9% of schoolchildren in 1990. Significantly more girls than boys were affected by fear of fatness and were trying to lose weight. CONCLUSION: A standard method for assessment of weight status is urgently needed for the evaluation of obesity prevention initiatives among Irish schoolchildren. Such initiatives need to be sensitive to the pervasiveness of fear of fatness among adolescent girls. 相似文献
99.
This paper reports a 3D-QSAR study using Catalyst software to explain the nature of interactions between flavor compounds and beta-lactoglobulin. A set of 35 compounds, for which dissociation constants were previously determined by affinity chromatography, was chosen. The set was divided into three subsets. An automated hypothesis generation, using HypoGen software, produced a model that made a valuable estimation of affinity and provided an explanation for the lack of correlation previously observed between the hydrophobicity of terpenes and the affinity for the protein. On the basis of these results, it appears that aroma binding to beta-lactoglobulin is caused by both hydrophobic interactions and hydrogen bonding, which plays a critical role. Catalyst appears to be a reliable tool for the application of 3D-QSAR study in aroma research. 相似文献
100.
Kurilich AC Jeffery EH Juvik JA Wallig MA Klein BP 《Journal of agricultural and food chemistry》2002,50(18):5053-5057
Antioxidant capacity of hydrophilic and lipophilic extracts from eight broccoli genotypes was compared using the oxygen radical absorbance capacity (ORAC) assay. Each genotype was analyzed for carotenoid, tocopherol, ascorbic acid, and flavonoid content. Results indicate that the antioxidant capacity of hydrophilic extracts ranged from 65.8 to 121.6 micromol trolox equivalents (TE)/g of tissue, and the capacity of lipophilic extracts ranged from 3.9 to 17.5 micromol TE/g. Ascorbic acid and flavonoid content of the hydrophilic extracts did not explain the total variation in antioxidant capacity of those extracts, suggesting either the presence of other antioxidant components that have yet to be identified or that the known antioxidants are producing synergistic effects. The carotenoids did correlate with antioxidant capacity of the lipophilic extracts and accounted for the majority of the variability in that fraction. The variability in hydrophilic and lipophilic antioxidant capacity found among these genotypes suggests that potential efficacy from antioxidants will vary considerably from genotype to genotype. 相似文献