Genome-wide analysis of plastome sequence variation and development of plastidial CAPS markers in common potato and related <Emphasis Type="Italic">Solanum</Emphasis> species

The plastome sequence of the European cultivated potato, Solanum tuberosum subsp. tuberosum (tbr, GenBank accession no. DQ386163), was compared with that of S. bulbocastanum, a wild potato relative (blb, GenBank accession no. DQ347958), in order to characterize the degree and type of variability in different genomic regions, and develop molecular markers relevant to genetics, breeding and biotechnology of potato. One hundred forty-two and 251 PICs (Potentially Informative Characters) were found in coding and non-coding sequences (NCSs), respectively. Further, while variation in coding regions was almost exclusively due to nucleotide substitutions, 25% of PICs in NCSs of tbr and blb were due to indels, most of them mononucleotide or longer tandem repeats (micro and minisatellites). Four intergenic regions were selected for further analyses in other 16 tuber-bearing Solanum species. The rps16-trnQ ^UUG gene spacer was found to be the most variable, forty-six PICs in this region distinguishing 18 haplotypes. Analysis of haplotype relationships, based on variability in the four intergenic regions, confirmed that the most primitive species from Central America were the most distant to S. tuberosum. Finally, polymorphic sites in the same regions were used to develop a set of CAPS (Cleaved Amplified Polymorphic Sequences) markers for species/cytoplasm identification in Solanum spp.     

Effect of harvest time on yield and pre-harvest quality of semi-leafless grain peas (Pisum sativum L.) as whole-crop forage

The aim of the work was to study changes in the yield and nutritional characteristics of whole crop semi-leafless field pea over two growing seasons in the Po plain, Italy. Samples of two cultivars (Baccara and Sidney) were collected from flowering to grain maturity. The developmental stage, yield, dry matter (DM) content, crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF), acid detergent lignin (ADL), starch, water soluble carbohydrates (WSC), gross energy (GE), organic matter digestibility (OMD) and the net energy for lactation (NE_L) were determined at each harvest. The forage characteristics were regressed on the growing degree days (GDD) with 4.4 °C as the base temperature. The DM yield increased with advancing maturity from 0.5 to 8.91 Mg ha⁻¹, while the CP decreased from 261 to 159 g kg⁻¹ DM. During the whole growth cycle the GE, OMD, NE_L and milk forage units (milk FU) were almost steady. No differences were observed between the cultivars for any of the measured parameters. At grain maturity, the crop produced over 4.0 Mg ha⁻¹ DM of grain. The CP, starch and WSC of the grain did not show any differences between the cultivars or years. The data showed that the nutritive quality of the forage of the semi-leafless grain pea harvested as a whole crop for ensiling purposes did not diminish with maturity and could help improve the self-sufficiency of dairy farms, in terms of home-grown protein forages.     

Antibacterial activity of coumarin as an innovative organic control strategy for Xanthomonas euvesicatoria pv. euvesicatoria

Journal of Plant Diseases and Protection - Bacterial spot caused by Xanthomonas euvesicatoria pv. euvesicatoria (Xanteu) is one of the major causes of yield losses in tomato (Lycopersicon...     

Biochemical Characterization of Cassiopea andromeda (Forsskål, 1775), Another Red Sea Jellyfish in the Western Mediterranean Sea

Increasing frequency of native jellyfish proliferations and massive appearance of non-indigenous jellyfish species recently concur to impact Mediterranean coastal ecosystems and human activities at sea. Nonetheless, jellyfish biomass may represent an exploitable novel resource to coastal communities, with reference to its potential use in the pharmaceutical, nutritional, and nutraceutical Blue Growth sectors. The zooxanthellate jellyfish Cassiopea andromeda, Forsskål, 1775 (Cnidaria, Rhizostomeae) entered the Levant Sea through the Suez Canal and spread towards the Western Mediterranean to reach Malta, Tunisia, and recently also the Italian coasts. Here we report on the biochemical characterization and antioxidant activity of C. andromeda specimens with a discussion on their relative biological activities. The biochemical characterization of the aqueous (PBS) and hydroalcoholic (80% ethanol) soluble components of C. andromeda were performed for whole jellyfish, as well as separately for umbrella and oral arms. The insoluble components were hydrolyzed by sequential enzymatic digestion with pepsin and collagenase. The composition and antioxidant activity of the insoluble and enzymatically digestible fractions were not affected by the pre-extraction types, resulting into collagen- and non-collagen-derived peptides with antioxidant activity. Both soluble compounds and hydrolyzed fractions were characterized for the content of proteins, phenolic compounds, and lipids. The presence of compounds coming from the endosymbiont zooxanthellae was also detected. The notable yield and the considerable antioxidant activity detected make this species worthy of further study for its potential biotechnological sustainable exploitation.     

Protection of farmed camels (Camelus Dromedarius): Welfare problems and legislative perspective

In the last years animal welfare has assumed an increasing interest in our society, influencing legislation to enact many provisions aimed at the protection of animals. Along with increased consumer awareness of the need to maintain ethically acceptable conditions of raised animals, scientists too have begun to investigate the conditions of animal welfare, the tools for its evaluation and for its improvement. Although there are many advances in knowledge, much remains to be investigated concerning many species considered "minor", that is, camels and dromedaries. Dromedaries, recently, have attracted the interest of some breeders following the results of studies concerning the nutritional and therapeutic properties of their products ‐ milk in particular ‐ that make them ideal for some particular categories of consumers, such as diabetics, obesity sufferers, lactose‐intolerant subjects, menopausal women and so on. Considering their use in dairy husbandry, dromedaries are reared under intensive and/or semi‐intensive systems with the resulting emergence of specific needs, which should be fulfilled in order to have appropriate welfare. This paper's purpose is to give practical elements in order to find out dromedary welfare standards, promoting a comprehensive set of regulations on welfare, care and protection of this animal.     

Does post‐anthesis heat stress affect plant phenology,physiology, grain yield and protein content of durum wheat in a semi‐arid Mediterranean environment?

Increasing air temperature due to changing climate is projected to decrease the length of the growing season, hasten vegetative development and maturation, and ultimately affect yield of many C3 crops. Previous multilocation trials highlighted strong relationships between thermal trends in the interval “beginning of flowering‐end of grain filling” and grain yield, and protein content in durum wheat (Triticum turgidum subsp. durum (Desf.) Husn.). With the aim to confirm these relationships, nine durum wheat genotypes, including old (Capeiti 8, Senatore Cappelli and Trinakria) and modern (Amedeo, Arcangelo, Mongibello, Simeto, and Svevo) varieties and a Sicilian landrace (Russello) were grown at three different sites representing a climate gradient in Sicily, Italy. Moreover, the effect of post‐anthesis heat stress on these durum wheats was further investigated in two contrasting environments: open‐field (control—C) and greenhouse heat stress (HS). HS shortened the interval “beginning‐end of flowering” of 1.5 days across genotypes, and the “end of flowering‐beginning of grain filling” and maturation of 4.9 days, with a range of 1 day in Arcangelo to 11 days in Cappelli. Advances in main phenophases significantly decreased kernel weight (KW) and grain yield (GY), whereas grain protein content (PC) increased. As expected, a negative relationship was observed between GY and PC, while positive relationships were found for GY and grain‐filling duration (GFD), and GY and KW. Genotypes responded differently to heat stress, as evidenced by the net photosynthesis, transpiration rate, instantaneous water use efficiency and dry matter accumulation in kernels. Genotypes were ranked according to the heat susceptibility index (HSI): Amedeo, Arcangelo, Capeiti 8, Svevo and Trinakria resulted heat‐tolerant. These varieties were characterized by an early trigger of dry matter accumulation in kernels under HS (Amedeo, Arcangelo and Trinakria), or showed similar length of the GFD (Capeiti 8) between environments. The multilocation trial confirmed a negative relationship between maximum temperatures and grain yield, and a positive relationship between minimum temperatures and protein content during grain–filling periods. Research focusing on agronomic strategies, phenology and breeding for tolerance to heat stress is of strategic importance to cope with the detrimental effect of global warming in semi‐arid climates.