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151.
152.
Giacomo Gnudi Antonella Volta Mattia Bonazzi Margherita Gazzola Giorgio Bertoni 《Veterinary radiology & ultrasound》2005,46(5):423-426
The aim of this study was to describe the ultrasonographic features of grass awns in soft tissue. A 10 MHz linear transducer was used. Ultrasound images from 25 dogs (27 awns) were collected and compared with the results from water bath studies using wild oat seeds (Avena spp.) collected in the field. Wild oat seeds were the most common grass awn found in soft tissue of dogs. Ultrasonographically grass awns appeared as a double/triple spindle-shaped echogenic interface within soft tissue. The same appearance was observed in water bath studies. In four dogs, the grass awn was removed surgically with a clamp introduced into a fistulous tract, using sonographic guidance. The grass awn was not found surgically in only three dogs, suggesting more attention during surgery. Ultrasonography is a useful diagnostic imaging technique to identify grass awns within soft tissue. 相似文献
153.
水牛活体采卵及体外受精研究 总被引:8,自引:0,他引:8
应用B型超声波采卵法进行水牛活体采卵,42头3~17岁良种水牛间隔3 d采卵1次,连续采卵2~3次,共进行96次,穿刺卵泡762个,回收卵母细胞513枚,其中可用卵母细胞305枚,回收率67.3%,可用卵占回收卵总数59.5%,平均每头次获卵母细胞5.34±4.25枚,其中可用卵母细胞3.18±2.89枚.采集的可用卵母细胞以MC和SOF 2种系统培养,受精分裂率和囊胚率分别为45.2%、30.6%和70.8%、18.8%.用8枚胚胎移植受体母牛8头,妊娠2头,产下了世界第1例由沼泽型水牛孕育生产的河流型水牛. 相似文献
154.
Massimo Zaccardelli Francesco Campanile Annalisa Spasiano Massimo Merighi 《European journal of plant pathology / European Foundation for Plant Pathology》2007,118(3):299-306
The development of a rapid detection method for Xanthomonas campestris pv. campestris (Xcc) in crucifer seeds and plants is essential for high-throughput certification purposes. Here we describe a diagnostic
protocol for the identification/detection of Xcc by PCR amplification of fragments from the pathogenicity-associated gene
hrcC. Under stringent conditions of amplification, a PCR product of 519 bp from hrcC was obtained from a collection of 46 isolates of Xcc, with the exception of two isolates from radish. No amplicons were obtained
from 39 pure cultures of the phytopathogenic bacteria Xanthomonas campestris pv. cerealicola, X. campestris pv. juglandis, X. campestris pv. pelargonii, X. campestris pv. vitians, X. arboricola pv. pruni,
X. axonopodis pv. phaseoli, X. axonopodis pv. vesicatoria, X. vesicatoria, Pseudomonas syringae pv. phaseolicola, P. syringae pv. syringae, P. syringae pv. tomato, P. fluorescens, P. marginalis, Pectobacterium atrosepticum, P. carotovorum subsp. carotovorum. In addition, PCR reactions were negative for fifty unidentified environmental isolates purified from the surface of crucifers.
The PCR fragment was obtained from four strains previously classified as X. campestris pv. aberrans, X. campestris pv. armorociae, X. campestris pv. barbarae and X. campestris pv. incanae using pathogenicity assays. Our PCR protocol specifically detected Xcc in inoculated leaves, seeds and naturally infected
leaves of crucifers. 相似文献
155.
Vito Montilon Angelo De Stradis Maria Saponari Raied Abou Kubaa Annalisa Giampetruzzi Giusy D'Attoma Pasquale Saldarelli 《Plant pathology》2023,72(1):144-153
Xylella fastidiosa subspecies pauca strain De Donno (XfDD) ST53 is the causal agent of olive quick decline syndrome, a severe disease first described in Apulia, Italy. Although the two local cultivars Cellina di Nardò and Ogliarola Salentina showed high susceptibility, traits of resistance to the bacterium were found in the cultivar Leccino. Previous studies in field-grown olives suggested that vascular occlusions and anatomophysiological properties of the different cultivars played a role in the olive response to XfDD. The present investigation reports observations at the early stage of the infection on artificially inoculated olives. Electron microscope studies showed that XfDD exploits the pit membranes (PMs) of the susceptible cultivar Cellina di Nardò to spread systemically. In this cultivar, PMs were degraded upon XfDD infection, suggesting activity of bacterial cell wall-degrading enzymes. Moreover, occluded vessels contained an amorphous electrondense matrix resembling gum. Conversely, in Leccino, occluded vessels were mainly filled by callose-like granules that tightly entrapped XfDD cells. In addition, PMs from Leccino had a compact undegraded structure that was not permeable to XfDD. Our study suggests that exploitation of PMs is a key event in the infection process of X. fastidiosa subsp. pauca ST53 in susceptible olive cultivars. 相似文献
156.
157.
158.
Salimei E Capilongo V Simoni A Peiretti PG Maglieri C Romano CA Mannina L Coppola R Sorrentino E 《Journal of agricultural and food chemistry》2007,55(23):9600-9607
The effects of Lactobacillus rhamnosus AT195, a potential probiotic microorganism cultured in buffalo "scotta" whey, on chemical and microbiological composition in maize and sorghum ensiling were evaluated. Both crops were harvested, chopped, and treated or not with the selected strain prior to ensiling in fiberglass vertical silos; 90 days after ensiling, silages were sensorially evaluated and sampled. Different chemical components were evaluated both on fresh crops and silages: in particular, the water-soluble carbohydrates content was investigated by high-field NMR spectroscopy and the carbohydrate fermentation profile was performed by GC. Besides phenotypic identification and typing, microbiological studies included Lb. rhamnosus genotype typing by RAPD-PCR. All silages, inoculated or not, were well preserved, as their chemical and microbiological data along with the fermentation profiles showed. The selected strain used as inoculum influenced the lactic acid population of silages and evidenced a good survival performance during the ensiling process of both maize and sorghum. Moreover, the use of Lb. rhamnosus strain efficiently improved the quality of the multifactorial ensiling process by significantly reducing the ammonia nitrogen content of both maize and sorghum silages. 相似文献
159.
160.
Francesca Truffa Giachet Claudia Vineis Diego Omar Sanchez Ramirez Riccardo Andrea Carletto Alessio Varesano Giorgio Mazzuchetti 《Fibers and Polymers》2017,18(4):720-730
Curcuma powder was used to dye cotton and polyamide 6,6 fabrics in order to produce textile-based optical pH sensors. Both fabrics showed a bright yellow color after dyeing and demonstrated color changes (towards red) when contacted with basic solutions. Color change and sensitivity differ for cotton and for polyamide. Curcuma-dyed cotton shows color changes in particular in the range of pH between 6.5 and 8.5, whilst curcuma-dyed polyamide shows a wider pH range: from 8.5 to 13.0. The stability of pH sensing to washing was evaluated. Three different kinds of washing agents were used in order to simulate the real life conditions of a garment or a cloth. Standard test methods were used when available for washing tests. The pH sensing of the curcuma-dyed fabrics demonstrated an excellent fastness to all kinds of washing. Ionic strength of the solution does not affect the color changes. Moreover, color reversibility of the fabrics was proven, too. Color change and reversibility of the fabrics was assessed by an UV-visible spectrophotometer. Spectral changes were observed at 540 nm for curcuma-dyed cotton, and at 487 and 574 nm for polyamide. 相似文献