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291.
292.
Ventura M Antonacci F Cardone MF Stanyon R D'Addabbo P Cellamare A Sprague LJ Eichler EE Archidiacono N Rocchi M 《Science (New York, N.Y.)》2007,316(5822):243-246
A systematic fluorescence in situ hybridization comparison of macaque and human synteny organization disclosed five additional macaque evolutionary new centromeres (ENCs) for a total of nine ENCs. To understand the dynamics of ENC formation and progression, we compared the ENC of macaque chromosome 4 with the human orthologous region, at 6q24.3, that conserves the ancestral genomic organization. A 250-kilobase segment was extensively duplicated around the macaque centromere. These duplications were strictly intrachromosomal. Our results suggest that novel centromeres may trigger only local duplication activity and that the absence of genes in the seeding region may have been important in ENC maintenance and progression. 相似文献
293.
Helena Gaspar Adele Cutignano Laura Grauso Nuno Neng Vasco Cachatra Angelo Fontana Joana Xavier Marta Cerejo Helena Vieira Susana Santos 《Marine drugs》2016,14(10)
Among marine organisms, sponges are the richest sources of pharmacologically-active compounds. Stemming from a previous lead discovery program that gathered a comprehensive library of organic extracts of marine sponges from the off-shore region of Portugal, crude extracts of Erylus cf. deficiens collected in the Gorringe Bank (Atlantic Ocean) were tested in the innovative high throughput screening (HTS) assay for inhibitors of indoleamine 2,3-dioxygenase (IDO) and showed activity. Bioassay guided fractionation of the dichloromethane extract led to the isolation of four new glycolipids, named erylusamide A–D. The structures of the isolated compounds were established by 1D and 2D nuclear magnetic resonance (NMR) spectroscopy, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) and chemical derivatization. The metabolites shared a pentasaccharide moiety constituted by unusual highly acetylated d-glucose moieties as well as d-xylose and d-galactose. The aglycones were unprecedented long chain dihydroxyketo amides. Erylusamides A, B and D differ in the length of the hydrocarbon chain, while erylusamide C is a structural isomer of erylusamide B. 相似文献
294.
295.
Gabriella Sonnante Anna Vittoria Carluccio Angelo De Paolis Domenico Pignone 《Genetic Resources and Crop Evolution》2008,55(7):1029-1046
A set of 24 microsatellite markers was identified in the artichoke genome, using various approaches. A genomic library allowed
the development of 14 SSR markers, whereas the other 10 were obtained from gene intron/UTR regions or from other species.
Allelic variation was scored in C. cardunculus (artichoke, cultivated cardoon, and wild cardoon) samples, and in other wild Cynara allies. For the 23 polymorphic loci, a total of 165 alleles were scored, 135 of which in the artichoke primary genepool,
and the remaining ones in the other Cynara species. Some allele combinations were able to identify artichoke varietal types, and some alleles were unique to specific
groups. This makes these markers potentially useful in product traceability and in contributing to the saturation of genetic
maps. The percentage of shared alleles between C. cardunculus taxonomic groups, and Nei’s genetic distances indicated that wild cardoons from the Eastern Mediterranean were more closely
related to artichoke and less to cultivated cardoon in comparison to wild cardoons from the Western Mediterranean, and the
genetic distance between the two wild cardoon genepools was rather high. The UPGMA dendrogram based on Nei’s genetic distances
revealed that artichokes formed a fairly defined cluster, whereas Eastern wild cardoons occupied another branch, and Western
wild cardoons were clustered together with cultivated cardoons. The transferability of microsatellite markers to other Cynara wild species was quite good. Sequencing alleles at three loci showed that, apart from microsatellite length variation, point
mutations and insertion/deletions were quite abundant especially when comparing C. cardunculus to the other Cynara species. In the sequenced regions, some SNPs were identified which distinguished artichoke on one side, and cultivated and
wild cardoon on the other, while other SNPs were apportioned according to the geographic distribution of Cynara wild species. 相似文献
296.
Lisboa AC Mello IC Nunes RS Dos Santos MA Antoniolli AR Marçal RM Cavalcanti SC 《Fitoterapia》2006,77(6):439-442
Oral administration of hexanes, chloroform, and ethyl acetate extracts of the leaves of Hyptis pectinata significantly reduced the number of writhing induced by acetic acid and increased the response to thermal stimuli in hot-plate test. Such effect was completely reversed by the opioid antagonist naloxone. 相似文献
297.
Stefania Loreti Amandine Cunty Nicoletta Pucci Aude Chabirand Emilio Stefani Adela Abelleira Giorgio M. Balestra Deirdre A. Cornish Francesca Gaffuri Davide Giovanardi Richard A. Gottsberger Maria Holeva Aynur Karahan Charikleia D. Karafla Angelo Mazzaglia Robert Taylor Leonor Cruz Maria M. Lopez Joel L. Vanneste Françoise Poliakoff 《European journal of plant pathology / European Foundation for Plant Pathology》2018,152(3):657-676
The aim of this study was to characterise the performance of new molecular methods for the detection and identification of Pseudomonas syringae pv. actinidiae (Psa) and to provide validation data in comparison to the assays mentioned in official diagnostic protocols and being currently used. Eleven molecular tests for the Psa detection were compared in an inter-laboratory comparison where each laboratory had to analyse the same panel of samples consisting of thirteen Psa-spiked kiwifruit wood extracts. Laboratories had to perform also isolation from the wood extracts. Data from this interlaboratory test performance study (TPS) was statistically analysed to assess the performance of each method. In order to provide complete validation data, both for detection and identification, this TPS was supplemented by a further study of identification from pure culture of phylogenetically closely related Pseudomonas spp., Psa, and bacterial strains associated with kiwifruit. The results of both these studies showed that simplex-PCRs gave good results, whereas duplex-PCR and real-time PCR were the most reliable tools for detection and identification of Psa. Nested and multiplex-PCR gave false-positive results. The use of the most reliable detection test is suggested for routine analyses, but when Psa-free status needs to be accurately assessed, it is recommended that at least two detection tests are used. This work provides a wide comparison of the available diagnostic methods, giving new information for a possible revision of the official diagnostic protocols (e.g. European and Mediterranean Plant Protection Organization (EPPO) protocol PM7/120 for the detection of Psa). 相似文献
298.
Pugliese Massimo Monchiero Matteo Gullino Maria Lodovica Garibaldi Angelo 《植物病害和植物保护杂志》2018,125(5):477-482
Journal of Plant Diseases and Protection - Erysiphe necator is favourably influenced by the increase in temperatures and remains the main pathogen threatening grape in Northern Italy. In the... 相似文献