The Role of Traditional Irrigation Canals in a Long Term Environmental Perspective A Case Study in Southern France： The Durance Basin

The agricultural sector is often considered to be the largest water consumer and the policy aimed at saving irrigation water exists across Europe. The flip-side of such policy, however, is the disappearance of traditional irrigation canals since farmers are encouraged to turn to drip irrigation and overhead irrigation gradually. Given this, we would like to argue that traditional irrigation canals need to be reexamined. Such canals are not only built aquatic infrastructure, they are also the product of a culture and social relationship with water in the Mediterranean region. Canals form a complex system which is driven by environmental, economic and social factors. Our sociological and environmental research in Provence （and around the Durance basin more specifically） points up how the very dense territorial network of gravity-fed canals is useful in water management, as well as in other shared uses over the long-term. The findings underscore the positive environmental role played by these historical and anthropogenic constructions. They contribute to refilling the aquifer and also act as a valuable environmental good and service （e.g., run-off regulation, biodiversity, landscape, recreation, etc.）. Further, several local actors have underscored their role as part of an intangible cultural heritage and as important for the area＇s economic and social development. Such canals play a central role in sustainable development since beyond their productive role in agriculture, they play a social role （new uses） and an ecological role （as reservoirs for biodiversity and ecological corridors）.     

Cassini magnetometer observations during Saturn orbit insertion

Cassini's successful orbit insertion has provided the first examination of Saturn's magnetosphere in 23 years, revealing a dynamic plasma and magnetic environment on short and long time scales. There has been no noticeable change in the internal magnetic field, either in its strength or its near-alignment with the rotation axis. However, the external magnetic field is different compared with past spacecraft observations. The current sheet within the magnetosphere is thinner and more extended, and we observed small diamagnetic cavities and ion cyclotron waves of types that were not reported before.     

Estimating prevalence of endometritis in smallholder zero-grazed dairy cows in Rwanda

Endometritis is a postpartum uterine disease of cows that interrupts reproductive cycles resulting in suboptimal fertility, reduced performance and profitability of the dairy herd. The objective of the study was to estimate the perceived and observed prevalence of endometritis among zero-grazed dairy cows in smallholder farms in Rwanda. A snowball sampling method was applied in cross-sectional survey to obtain data from 370 farms on 466 cows within their 21–60 days postpartum (dpp). The survey, conducted from September 2018 to March 2019, simultaneously examined cows using the Metricheck Device (MED) to determine the presence and type of vaginal mucus (VMC) based on a score scale of 0 to 3. Cows scoring VMC ≥ 1 were recorded as clinical endometritis (CLE)–positive. Cytotape (CYT) was used to determine the percentages of polymorphonuclear cells (PMN) in endometrial cytology sample. Cows with ≥ 5% PMN were recorded subclinical endometritis (SCLE)–positive, whereas cows with VMC-0 and < 5% PMN were considered healthy cows. At cow-level, endometritis prevalence was 70.2% with 67.2% CLE and 31.8% SCLE while at the herd-level, prevalence was 71.1% with 68.1% CLE and 34.4% SCLE. The differences between the diagnostic performance of the MED and CYT were significant (p < 0.001). Perceived prevalence by farmers was much lower (3.2%) and without agreement with the observed prevalence (kappa = − 0.02, p > 0.05). The highly observed prevalence and farmer underestimation of endometritis prevalence indicate knowledge gaps about endometritis. The extension service therefore needs to increase awareness and education among smallholder farmers about detection and management of endometritis.

     

Biocontrol of soil diseases and soil profile associated with rhizosphere of rice (Oryza sativa Subsp. Japonica) growing paddy fields in Kansai region,Japan

ABSTRACT

This research focused on in vitro and in vivo biocontrol of two major rice diseases, Bacterial Leaf Blight caused by Xanthomonus oryzae pv oryzae (Xoo) and Sheath Blight caused by Rhizoctonia solani. Furthermore, the soil of paddy fields in Kansai region, Japan was analysed for the presence of these pathogens, and their microbial communities were evaluated according to the location and management systems. For biocontrol, the rhizospheric bacteria were isolated and molecularly identified. The results indicated that DAR17225040 and DAR17225017 were 99% similar to Bacillus aryabhattai and Bacillus megaterium. The isolated bacteria were able to suppress Bacterial Leaf Blight and Sheath Blight disease 78% and 86% respectively, under greenhouse conditions. The 16S amplicon sequencing (Illumina MiSeq 250bp) was used to analyse the bacterial community in paddy fields of soil taken from five locations under conventional and organic systems. Xoo and R. solani were detected in sampled soil, however, no clear difference was noted, regarding systems. From amplicon sequencing data, Chloroflexi, Acidobacteria, Proteobacteria, Actinobacteria, Planctomycetes were the dominant phyla of rice soil in Kansai region. The phylum Kazan-3B-28 was found more in organic system than conventional. The bacterial community was more affected by location and soil type than by management systems.     

Laparoscopic-assisted colopexy and sterilization in male dogs: short-term results and physiologic consequences

Objective: To describe laparoscopic‐assisted colopexy and sterilization, and evaluate outcome and effects in healthy male dogs. Study Design: Experimental study. Animals: Male Beagle dogs (n=7). Methods: A laparoscopic‐assisted, extracorporeally sutured colopexy, and sterilization by ligation and section of the testicular vessels and ductus deferens were evaluated 11 weeks after surgery. Ex vivo tensile tests were performed on the colopexy sites and loss of testicular function was assessed by monitoring serum testosterone, and by ultrasonographic and histologic examinations of the testes. Systemic inflammation and potential iatrogenic colonic functional disorders were investigated by monitoring serum C‐reactive protein (CRP) in the perioperative period and from a sulfapyridine (SP) kinetic profile obtained before and 10 weeks after surgery. Results: No intraoperative complications were recorded and clinical outcome was considered fair in all dogs. A mean tensile force of 42 N was required to disrupt colopexies. No relevant postoperative increase in CRP concentrations or changes in SP kinetics were observed. Testicular function was lost. Conclusions: Laparoscopic‐assisted colopexy achieved adhesion of the colon to the abdominal wall and testicular endocrine function and spermatogenesis were eliminated by laparoscopic castration.     

Cloning and characterization of 2S albumin, Car i 1, a major allergen in pecan

Although pecans are associated with IgE-mediated food allergies, the allergens responsible remain to be identified and characterized. The 2S albumin gene was amplified from the pecan cDNA library. Dot-blots were used to screen the recombinant protein with pecan allergic patients' serum. The affinity purified native protein was analyzed by Edman sequencing and mass spectrometry/mass spectrometry (MS/MS) analysis. Cross-reactivity with walnut was determined by inhibition enzyme-linked immunosorbent assay (ELISA). Sequential epitopes were determined by probing the overlapping peptides with three different patients' serum pool. The 3-dimensional homology model was generated, and the locations of the pecan epitopes were compared with those of known sequential epitopes on other allergenic tree nut homologues. Of 28 patients tested by dot-blot, 22 (79%) bound to 2S albumin, designated as Car i 1. Edman sequencing and the MS/MS sequencing of native 2S albumin confirmed the identity of recombinant (r) Car i 1. Both pecan and walnut protein extracts inhibited the IgE-binding to rCar i 1. Sequential epitope mapping indicated weak, moderate, and strong reactivity against 12, 7, and 5 peptides, respectively. Of the 11 peptides recognized by all serum pools, 5 peptides were strongly reactive and located in 3 discrete regions of the Car i 1 (amino acids 43-57, 67-78, and 106-120). Three-dimensional modeling revealed IgE-reactive epitopes to be solvent accessible and share significant homology with other tree nuts providing a possible basis for previously observed cross-reactivity.