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91.
Detection of Antibodies Against Peste des Petits Ruminants Virus in Sera of Cattle,Camels, Sheep and Goats in Sudan 总被引:1,自引:0,他引:1
Detection of antibodies against peste des petits ruminants virus in sera of cattle, camels, sheep and goats in Sudan. 相似文献
92.
Ilhami Kiziro?lu Levend Turan Ali Erdo?an ?zdemir Adizel Nahit Pamuko?lu 《Anzeiger für Sch?dlingskunde》2002,37(1):99-102
Analysis of nestling food in four species of Parus and one species of Ficedula using the 'Halsringmethode' showed a high quota of adults and caterpillars of the important forest pests: Tortrix viridana, Euproctis chrysorrhoea, Diprion pini, and Lymantria dispar. These four major pest species amounted in the nestling food of Parus major to 50%, P. coeruleus to 52.6%, P. ater to 40.9%, and Ficedula hypoleuca to 30.2%. 相似文献
93.
The fluorescent antibody (FA) assay was developed for detecting the stunting syndrome agent (SSA) from intestinal tissue. Similarly, the indirect fluorescent antibody (IFA) assay was developed for detecting serum antibodies to SSA. Convalescent antiserum from turkeys orally immunized with SSA was found to be the primary antibody of choice for the FA assay. Intestinal jejunal samples from poults inoculated 3-4 days postinoculation (DPI) was found to be the best antigen source for the IFA assay. SSA was detected from the intestinal tracts of experimentally inoculated birds at 2 DPI with highest level of reactivity at 3 DPI by the FA assay. After 4 DPI the level of SSA infectivity of the intestines waned to low levels. Serum antibody was detected from experimentally inoculated birds as early as 7 DPI with all birds tested seroconverting by 12 DPI. These assays should prove useful for future studies concerning stunting syndrome. 相似文献
94.
试验选用约2.5岁公牦牛8头,在三江源地区玉树县的高寒草甸草场,应用4N盐酸不溶灰分法(4N—AIA)和木质素标记物法测定了不同物候期(青草期和枯黄期)放牧牦牛日采食量及牧草营养成分的消化率。结果表明:2.5岁牦牛在青草期、枯黄期的100kg体重日采食量分别为3.69kg和2.96kg,差异极显著(P〈0.01);其不同物候期的千物质消化率分别为65.70%、61.72%(P〉0.05),粗蛋白的消化率为62.25%、39.00%(P〈0.01),粗脂肪为49.36%、58.18%(P〈0.05),ADF的消化率为57.88%、62.67%(P〉0.05),NDF的消化率为69.09%、79.38%(P<0.01)。 相似文献
95.
96.
Genetic inheritance of resistance to Bacillus thuringiensis var kurstaki (BTK) was examined in a diamondback moth (Plutella xylostella) population collected from the Melaka region of Malaysia. A BTK‐selected sub‐population (BTK‐SEL) which was more than 100‐fold resistant to BTK compared with a susceptible (ROTH) population of P xylostella was used with standard reciprocal crosses and back‐crosses between ROTH and BTK‐SEL. Logit regression analysis of F 1 reciprocal crosses indicated that BTK resistance was inherited as an incompletely recessive autosomal trait and controlled by a single locus. In contrast, other studies have shown that resistance to Cry1Ac is inherited as an incompletely dominant autosomal trait in a Cry1Ac‐selected sub‐population of the same Melaka population. The frequency of the allele responsible for resistance decreased without exposure to insecticide in the laboratory. © 2000 Society of Chemical Industry 相似文献
97.
AGPT and HA tests were employed for rapid diagnosis of PPRV infection in sheep and goats in Sudan. Forty lymph nodes and spleen
samples from suspected cases of PPR in both sheep and goats were examined by AGPT and HA tests for detection of PPRV antigen.
Viral antigen was detected from (77.5%) of the samples tested by AGPT and (92.5%) tested by HA test. The results of both tests
revealed that HA test was more sensitive than AGPT for detection of PPRV antigen (Kappa statistics 0.4366). Another advantage
of the HA test over AGPT was that it can differentiate PPRV from RPV. Thus the HA test represents a quick, easy, simple, cheap
and reliable confirmatory test for the diagnosis of PPR and differential diagnosis of PPRV and RPV. The HA test was carried
out using chicken, goat and pig RBCs. Chicken RBCs were found to be the most sensitive for detection of PPRV antigen, followed
by goat then pig RBCs. The HA time when using chicken RBCs was 20–25 minutes, using goat RBCs was 25–30 minutes and using
pig RBCs was 40–45 minutes. The distribution of PPR infection in four different regions of Sudan was investigated. 相似文献
98.
Abstract In an attempt to identify appropriate feeding rates for multispecies of fish raised in fertilized earthen ponds, the present work was conducted over a 19‐week experimental period to establish the growth performance, production and body composition of Nile tilapia, common carp and silver carp fed 0.0%, 0.5%, 1.0%, 3.0%, 5.0% biomass and to apparent satiation (treatments). Twelve ponds were stocked with a similar number and weight of each fish species. Two ponds were assigned to each of the treatments, and a 25% protein pelleted fish feed was used to feed fish at the specified rate of feeding. At the end of the experiment, growth, weight gain, survival, yield and body composition of fish groups were affected by the treatments. The economic effectiveness also varied among treatments. The most conspicuous attribute of the feeding rates was its lack of influence on growth (g day?1), weight gain (g per fish), yield (kg ha?1) or body composition of silver carp. The results of whole‐body proximate analysis indicated that various feeding rates had either an irregular pattern or no effects on the protein and ash gain per 100 g of fish body weight (bw) gain. The most notable exceptions were significant (P < 0.05) increases in body fat and gross energy gains in Nile tilapia, common carp and silver carp accompanied by decreases in percentages of moisture (but not in silver carp) as feeding rate increased. Among the six different feeding levels, feeding to apparent satiation (feed amount was equivalent to 2.67% of fish bw day?1) appeared to be optimal, as it significantly (P < 0.05) supported the highest fish production, income and net profit compared with all other treatments except for the 3% feeding level, for which the differences in those measurements were comparable. 相似文献
99.
用选自陆海杂交后代的抗蚜选系抗77为母本与具中棉亲缘的丰产品种江苏棉1号杂交,后代与父本江苏棉1号回交2次,经20代综合鉴定和多性状定向选育的川棉109。该品种抗棉蚜,抗枯萎病和立枯病,耐黄萎病,耐旱,抗倒,耐瘠薄;并克服了陆地棉密茸毛与短纤维的连锁,将抗虫与抗病、丰产与优质有机组合;综合性状鉴定为国内领先和国际先进水平.目前己累积推广27万hm2,是利用多质资源培育多抗型丰产品种的实例。多质资源是前提,多抗性、强生长势、强补偿力等构成了川棉109丰产稳产的综合遣传优势。启示育种目标日趋综合的现代棉花育种应选择宽广的质源,培育多抗性取代单抗性,并以胁迫和非胁迫双重条件下的产量比较为核心,实现抗性与丰产性的统一。 相似文献
100.
Identification of QTLs for rice grain size and shape of Iranian cultivars using SSR markers 总被引:6,自引:0,他引:6
Grain size is a main component of rice appearance quality. In this study, we performed the SSR mapping of quantitative trait loci (QTLs) controlling grain size (grain length and breadth) and shape (length/breadth ratio) using an F2 population of a cross between two Iranian cultivars, Domsephid and Gerdeh, comprising of 192 individuals. A linkage map with 88 markers was constructed, which covered 1367.9 cM of the rice genome with an average distance of 18 cM between markers. Interval mapping procedure was used to identify the QTLs controlling three grain traits, and QTLs detected were further confirmed using composite interval mapping. A total of 11 intervals carrying 18 QTLs for three traits were identifed, that included five QTLs for grain length, seven QTLs for grain breadth, and six QTLs for grain shape. A major QTL for grain length was detected on chromosome 3, that explained 19.3% of the phenotypic variation. Two major QTLs for grain breadth were mapped on chromosomes 3 and 8, which explained 34.1% and 20% of the phenotypic variation, respectively. Another two major QTLs were identified for grain shape on chromosomes 3 and 8, which accounted for 27.1% and 20.5% of the phenotypic variance, respectively. The two QTLs that were mapped for grain shape coincided with the major QTLs detected for grain length and grain breadth. Intrestingly, gs2 QTL specific to grain shape was detected on chromosome 2 that explained 15% of the phenotypic variation. 相似文献