盐藻丝氨酸/苏氨酸蛋白激酶基因DsSTPK的克隆、原核表达及纯化

前期研究表明,盐藻丝氨酸/苏氨酸蛋白激酶DsSTPK在转录水平上的表达受盐胁迫诱导。为了进一步研究该蛋白激酶的生理功能,通过RT-PCR扩增DsSTPK的开放阅读框,并将其克隆至pET32a(+)载体,得到重组表达载体pET32a(+)-DsSTPK。将重组表达载体转化E. coli. BL21 (DE3),IPTG诱导表达,然后对融合蛋白进行表达形式分析,利用His60 Ni离子重力柱进行纯化,所得产物进行SDS-PAGE和Western-blot鉴定。本研究成功构建了重组表达载体pET32a(+)-DsSTPK,经IPTG诱导后表达出的融合蛋白的分子量与预期相符;表达形式分析显示该融合蛋白在上清和包涵体中均存在;将上清蛋白纯化后获得了纯度较高的融合蛋白;Western-blot检测显示该融合蛋白能被抗组氨酸抗体特异性识别,具有良好的免疫学活性。DsSTPK的成功表达、纯化,为下一步制备抗体,然后进一步在蛋白质水平上研究其在盐藻耐盐机制中的作用奠定了基础。     

Expression profiling of transgenes (Cry1Ac and Cry2A) in cotton genotypes under different genetic backgrounds

Transgenic cotton carrying the CrylAc gene has revolutionized insect pest control since its adoption,although the development of resistance in insect pests has reduced its efficacy.After 10 years of cultivating Bacillus thuringiensis(Bt) cotton with a single Cry1 Ac gene,growers are on the verge of adopting Bt cotton that carries the double gene(Cry1 Ac+Cry2 A) due to its better effectiveness against insect pests.Thus,the current study was designed to evaluate the role of each gene in the effect...     

联合收割机割台总成的虚拟装配

研究了基于虚拟现实技术的联合收割机割台总成的虚拟装配.根据产品的装配结构模型,生成了联合收割机割台总成的装配结构树;采用UG建立了割台总成零部件的三维数字模型,导入到数字制造仿真软件,建立了总成各零部件的约束及装配关系,对割台总成进行了虚拟装配.     

高速部分流泵CFD计算和优化设计

为研究高速部分流泵中流量系数、扬程系数和比转速三者之间的关系,对该泵主要参数进行了优化设计.在水泵性能试验的基础上,采用Matlab软件对试验数据进行数值拟合,推导出三者的函数关系式,并对主要性能参数进行了精确计算.将优化后的参数应用于Pro/E建模,运用ANSYS-CFX进行了数值计算和外特性试验.结果表明,中间截面静压呈环状梯度由轮毂向轮缘逐渐递增,出口静压满足优化要求;流线云图中虽然存在漩涡,但隔舌处的回流和涡流得到了明显的改善;从扬程-流量试验曲线也可以看到,在从小流量到设计工况点间运行非常平稳.该结果为实际工程应用提供了一定参考.     

膜上苗带覆土装置设计与试验研究

在花生机械化播种过程中,膜上苗带覆土的性能直接影响到播种的质量,进而影响到花生的产量.该文基于2BFD-2C型花生覆膜播种机,根据花生种植农艺要求和膜上苗带覆土的技术要求,设计了膜上苗带覆土装置.该装置主要由覆土圆盘、覆土滚筒、漏土缝隙、挡土板和导土板等组成,可一次性完成覆土压膜,横向送土,膜上覆土形成筑土带等作业.该文通过试验确定了各零部件的形式和最优结构参数,进一步提高了播种效率和播种质量,为多功能花生覆膜播种机的膜上苗带覆土装置的设计与应用奠定了一定的理论基础.     

荒漠化地区农村能源利用现状及建议

合理解决荒漠化地区的农村能源利用与生态和环境的矛盾,实现多元化能源的合理利用,对于促进中国的社会主义新农村建设,实施可持续发展战略有着重要的意义。详细介绍内蒙古阿拉善左旗铁木日乌德嘎查的现状及能源利用基本情况,从社会经济、技术、生产结构、人文和自然地理等角度对能源利用情况进行了多方面的分析,找出了决定该嘎查采用当前的能源利用方式和结构的原因及影响因素。当前这种以玉米芯、秸秆、煤、电、柴油、汽油为主,液化石油气和太阳能为辅的生产已及生活用能结构不仅使当地居民饱受经济和健康的双重压力,同时也对当地荒漠化的进程起到了一定程度的推波助澜的作用。针对上述问题,分别从传统能源和新能源以及非政府组织的作用等方面给出了几点建议。     

Wicking properties of polyamide 66 twisted nanofiber yarn by tracing the color alteration in yarn structure

In view of the interest in wicking properties of these flexible structures, analysis of the wicking phenomena in nylon 6.6 nanofiber yarns is carried out by considering the twist rate effects. A novel method is used based on adding a pH-sensitive dye to yarn interstructure and the analysis of color alteration of nanofiber yarn structure, resulting from a shift in pH, during the capillary rise of distilled water. The results show that the addition of pH- sensitive dye has no influence on the average nanofiber diameter and the wicking behavior of yarns. This study shows that in short durations, the kinetic of the capillary rise follows the Lucas-Washburn equation. The Lambertw, a mathematical function, has been incorporated, which helps measure an equivalent structural factor of nanofiber yarns and vertical wicking height at any given time considering the gravitational effects. The statistical results show that the average of equilibrium wicking height and capillary rise rate coefficient tend to decrease with increasing the nanofiber yarn twist, due to the reduction of continuity and size of capillaries.     

能源植物斑茅在不同生长时期的产量与组分变化

研究不同时期斑茅产量及其化学组分的变化。结果表明:斑茅生物质产量在11月达到最高,其后开始下降;整个生长发育过程中茎叶生物质产量比呈现上升趋势;含水量随生长发育的进行显著降低;叶片灰分含量全年最低为5月,从10月份开始显著增加,至12月份达到最高。茎秆灰分含量略低于叶片,全年最低为10月,最高为7月,变化幅度不大。叶片中木质素含量全年由高到低排列顺序为12月11月9月10月7月8月6月;茎秆中木质素含量全年由高到低排列顺序为12月11月9月7月8月10月6月。10月份茎秆含水量低,灰分含量最低,热值较高,结合产量和燃料品质考虑其更适于直接燃烧,而9月份叶片则更加适于作为生物转化的材料。     

Effect of oleuropein on tissue myeloperoxidase activity in experimental spinal cord trauma

Background: Neutrophil infiltration plays an important role in inflammatory reactions following spinal cord injury (SCI) and these cells cause substantial secondary tissue damage. The purpose of this study was to determine the effect of oleuropein (OE) on myeloperoxidase (MPO) activity as an index of neutrophil infiltration. Methods: Rats were randomly divided into four groups of 7 rats each as follows: sham-operated group, trauma group, and OE treatment groups (20 mg/kg, i.p., immediately and 1 hour after SCI). Spinal cord samples were taken 24 hours after injury and studied for determination of MPO activity. Results: The results showed that MPO activity was significantly decreased in OE-treated rats. Conclusion: On the basis of our findings, we propose that OE may be effective in protecting rat spinal cord from secondary damage by modulating of neutrophil infiltration.Key Words: Oleuropein (OE), Neutrophil infiltration, Myeloperoxidase     

Comparative Assessment of Neutrophil Gelatinase-Associated Lipocalin (NGAL) and Cystatin C as Early Biomarkers for Early Detection of Renal Failure in Patients with Hypertension

Background:

Hypertension is one the most common causes of chronic kidney disease (CKD). One of the major concerns in hypertensive patients is early detection of renal disorders. In the past, serum creatinine (Scr) concentration was used as a marker of kidney function, but it proffers a late reflection of reduced glomerular filtration rate. Cystatin C and neutrophil gelatinase-associated lipocalin (NGAL) have been recently proven to be useful for quantification of CKD. Therefore, we compared the diagnostic value of NGAL with cystatin C and creatinine to evaluate kidney function in hypertensive patients.

Methods:

In this study, 42 hypertensive patients and 30 healthy volunteers were recruited. Serum cystatin C (Scys C) and plasma NGAL were measured using ELISA method. Creatinine, urea, hemoglobin, fibrinogen, and C-reactive protein were measured according to the routine methods. Estimated glomerular filtration rate (eGFR) was considered as the gold standard method (cut-off value of < 78 ml/min/1.73 m².

Results:

In the patient group, plasma NGAL, cystatin C, and creatinine were all significantly correlated with eGFR, and plasma NGAL correlated best with eGFR. Receiver-operating characteristics analysis indicated that plasma NGAL was a better indicator than creatinine and cystatin C for predicting a GFR < 78 ml/min/1.73 m². The sensitivity and specificity for NGAL were 96% and 100%, for cystatin C were 92% and 60% and for creatinine were 76% and 47%, respectively.

Conclusion:

Plasma NGAL demonstrated a higher diagnostic value to detect kidney impairment in the early stages of CKD as compared to Scys C and Scr in hypertensive patients.Key Words: Neutrophil gelatinase-associated lipocalin (NGAL), Cystatin C, Creatinine, Hypertension