全文获取类型
收费全文 | 387篇 |
免费 | 20篇 |
专业分类
林业 | 25篇 |
农学 | 11篇 |
93篇 | |
综合类 | 50篇 |
农作物 | 25篇 |
水产渔业 | 37篇 |
畜牧兽医 | 128篇 |
园艺 | 13篇 |
植物保护 | 25篇 |
出版年
2023年 | 3篇 |
2022年 | 3篇 |
2021年 | 18篇 |
2020年 | 21篇 |
2019年 | 16篇 |
2018年 | 10篇 |
2017年 | 17篇 |
2016年 | 27篇 |
2015年 | 16篇 |
2014年 | 14篇 |
2013年 | 11篇 |
2012年 | 34篇 |
2011年 | 42篇 |
2010年 | 15篇 |
2009年 | 20篇 |
2008年 | 18篇 |
2007年 | 24篇 |
2006年 | 16篇 |
2005年 | 16篇 |
2004年 | 22篇 |
2003年 | 10篇 |
2002年 | 16篇 |
2001年 | 1篇 |
2000年 | 2篇 |
1998年 | 1篇 |
1995年 | 2篇 |
1993年 | 2篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1983年 | 1篇 |
1981年 | 1篇 |
排序方式: 共有407条查询结果,搜索用时 15 毫秒
61.
Ballabio C Magni C Restani P Mottini M Fiocchi A Tedeschi G Duranti M 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(4):396-402
The immunological cross-reactivity among major protein- and oil-crops, including lupin, lentil, pea, peanut, kidney bean and
soybean, has been studied by a combination of in vitro and in vivo experimental approaches: SDS-PAGE separations of legume protein extracts and immuno-blot revelations with 12 peanut-sensitive
subjects’ sera, Immuno-CAP and Skin Prick tests on the same subjects. The immuno-blotting data showed a wide range of IgE-binding
responses both displayed by one subject towards different plant extracts and among subjects. Differences were both quantitative
and qualitative. The prevalent responses of most subjects’ sera were seen with peanut polypeptides, as expected, as well as
with various polypeptides of the other legumes, the most recurrent of which were the basic subunits of the 11S globulins.
The distribution of in vivo responses generally paralleled those obtained by in vitro approaches with strong responses elicited by peanut, lentil and pea protein extracts, especially by most sensitive subjects,
thus providing a consistent overall set of results. In this work, the comparison of various approaches has allowed us to get
an overall broad picture of the immunological cross-reactivities among proteins of widely used different seed species and
to hypothesize the role of most conserved specific polypeptides. 相似文献
62.
Pereira MC Steffens RS Jablonski A Hertz PF Rios Ade O Vizzotto M Flôres SH 《Journal of agricultural and food chemistry》2012,60(12):3061-3067
The objective of this study is to evaluating the Brazilian biodiversity through physicochemical characterization and determination of antioxidant potential of three species from the Myrtaceae family, namely yellow guava (Psidium cattleyanum Sabine), guabiroba (Campomanesia xanthocarpa O. Berg), and uvaia ( Eugenia pyriformis Cambess). Guabiroba had the greater quantity of phenolic compounds (9033 mg chlorogenic acid/100 g) and vitamin C (30.58 mg/g) and showed the best TSS/TTA (total soluble solid/total titratable acid) ratio (45.12). For the ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic) method, the guabiroba (507.49 μM Trolox/g) presented the highest antioxidant potential; however, in the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, uvaia (170.26 g/g DPPH) and guabiroba (161.29 g/g DPPH) were not statistically different. The uvaia outranked the other fruits with respect to its high carotenoid (909.33 μg/g) and vitamin A (37.83 μg/g) contents, and the yellow guava, although showing a lower bioactive compound content and antioxidant activity, nevertheless presented much higher values than many traditionally consumed fruits. 相似文献
63.
Daniela Gargano Nunzia Scotti Alessandro Vezzi Alessandra Bilardi Giorgio Valle Stefania Grillo Salvatore Cozzolino Teodoro Cardi 《Genetic Resources and Crop Evolution》2012,59(3):419-430
The plastome sequence of the European cultivated potato, Solanum tuberosum subsp. tuberosum (tbr, GenBank accession no. DQ386163), was compared with that of S. bulbocastanum, a wild potato relative (blb, GenBank accession no. DQ347958), in order to characterize the degree and type of variability
in different genomic regions, and develop molecular markers relevant to genetics, breeding and biotechnology of potato. One
hundred forty-two and 251 PICs (Potentially Informative Characters) were found in coding and non-coding sequences (NCSs),
respectively. Further, while variation in coding regions was almost exclusively due to nucleotide substitutions, 25% of PICs
in NCSs of tbr and blb were due to indels, most of them mononucleotide or longer tandem repeats (micro and minisatellites).
Four intergenic regions were selected for further analyses in other 16 tuber-bearing Solanum species. The rps16-trnQ
UUG
gene spacer was found to be the most variable, forty-six PICs in this region distinguishing 18 haplotypes. Analysis of haplotype
relationships, based on variability in the four intergenic regions, confirmed that the most primitive species from Central
America were the most distant to S. tuberosum. Finally, polymorphic sites in the same regions were used to develop a set of CAPS (Cleaved Amplified Polymorphic Sequences)
markers for species/cytoplasm identification in Solanum spp. 相似文献
64.
Costagliola A Britti D Russo V Meomartino L Castagna F Giordano D Insabato L Paciello O 《Avian diseases》2011,55(1):147-150
A common seagull (Larus fuscus) was found near the southern coast of Italy by the veterinarians of the local wild animal rescue center. Physical examination of the bird revealed an ulcerated mass involving a majority of the oral cavity; the mass did not allow for normal feeding. After the bird died necropsy was performed and the mass was histologically and immunohistochemically examined. The morphology and the immunoreactivity for Melan-A and S-100 antigens led to a diagnosis of malignant melanoma. This is the first case of malignant melanoma described in a seagull, and herein we compare the characteristics of the present case with malignant melanoma already described in domestic animals. 相似文献
65.
66.
Berlanda M Zotti A Brandazza G Poser H Calò P Bernardini M 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(12):1334-1338
Magnetic resonance and computed tomography features of 4 cases of canine congenital vertebral anomalies (CVAs) are discussed. Two of the cases represent unusual presentations for such anomalies that commonly affect screw-tail or toy breeds. Moreover, the combination of CVAs and a congenital peritoneo-pericardial diaphragmatic hernia has never before been imaged. 相似文献
67.
Ribeiro MG Takai S Guazzelli A Lara GH da Silva AV Fernandes MC Condas LA Siqueira AK Salerno T 《Research in veterinary science》2011,91(3):478-481
The virulence genes and plasmid profiles of 23 Rhodococcus equi isolates from 258 lymph nodes from domestic pigs (129 nodes with lesions and 129 without lesions) and 120 lymph nodes from slaughtered wild boars (60 nodes with lesions and 60 without) were characterized. R. equi was obtained from 19 lymph nodes of domestic pigs, 17 with, and two without lesions, and from four lymph nodes with lesions, from wild boars. The 23 isolates were tested for the presence of vapA and vapB genes, responsible for the 15–17 and 20 kDa virulence-associated proteins, respectively, by PCR in order to characterize as virulent (VapA), intermediately virulent (VapB) and avirulent. Plasmid DNAs were isolated and analyzed by digestion with restriction endonucleases to estimate size and compare their polymorphisms. Of the 19 domestic pigs strains, seven (36.8%) were avirulent and 12 (63.2%) were intermediately virulent, with the intermediately virulent isolates being plasmid types 8 (8 isolates), 10 (2 isolates), 1 (1 isolate) and 29 (1 isolate). The plasmid type of four strains isolated from wild boars was also intermediately virulent type 8. None of the domestic pigs and wild boar isolates showed the vapA gene. These findings demonstrate a high occurrence of plasmid type 8 in isolates from pigs and wild boars, and the similarity of plasmid types in the domestic pigs, wild boars and human isolates in Brazil. 相似文献
68.
Gustavo Henrique Batista Lara Clarice Queico Fujimura Leite Alessandro Guazzelli Adolfo Carlos Barreto Santos 《Research in veterinary science》2011,90(2):185-188
Mycobacterium spp. and other pathogens were investigated in 258 swine lymph nodes (129 with and 129 without apparent lesions), and 120 lymph nodes (60 with and 60 without lesions) from wild boars (Sus scrofa). A total of lymph nodes from swine and wild boars were collected of different animals. Submaxillar and mesenteric lymph nodes were submitted to microbiological examination and colonies suggestive of Mycobacterium spp. (alcohol-acid bacilli) were submitted to PCR Restriction Assay (PRA). In swine with lymphadenitis, Mycobacterium spp. (24.1%) and Rhodococcus equi (13.2%) were the most prevalent microorganisms, while in lymph nodes without lesions were identified a complex of microorganisms, including of environmental mycobacteria. In wild boars with lymphadenitis, ß-haemolytic Streptococcus (10.0%), Mycobacterium spp (8.4%) and R. equi (6.6%) were the most frequent. Among mycobacterias were identified predominantly Mycobacterium avium subspecies type 1 (48.3%) and M. avium subspecies type 2 (16.1%), followed by Mycobacterium intracellulare, Mycobacterium szulgai,Mycobacterium fortuitum, Mycobacterium gordonae, Mycobacterium simiae, Mycobacterium nonchromogenicum and Mycobacterium intracellulare type 2. 相似文献
69.
70.
Michele R. S. Simões Joal J. B. Leal Alessandro P. Minho Cláudia C. Gomes Michael D. MacNeil Rodrigo F. Costa Vinícius S. Junqueira Patrícia I. Schmidt Fernando F. Cardoso Arione A. Boligon Marcos J. Yokoo 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2020,137(2):177-188
Our goal was to define a breeding objective for Brangus beef cattle in Brazil. Bioeconomic models were produced and used to estimate economic values (EVs). The scenarios simulated were typical full-cycle beef production systems that are used in tropical and subtropical regions. The breeding objective contained pregnancy rate (PR), warm carcass weight (WCW), mature cow weight (MCW), number of nematode eggs per gram of faeces (EPG) and tick count (TICK). Two models were used in series to estimate the EV. A deterministic model was used to simulate effects of PR, WCW and MCW on profitability with a constant parasite load. Subsequently, stochastic models were used to estimate economic values for TICK and EPG as consequences of their environmental effects on weight gains, mortality and health costs. The EV of PR, WCW, MCW, EPG and TICK, was US$1.59, US$2.11, −US$0.24, −US$5.35 and −US$20.88, respectively. Results indicate positive emphasis should be placed on PR (12.49%) and WCW (65.07%) with negative emphasis on MCW (13.92%), EPG (2.77%) and TICK (5.75%). In comparison with the indexes usually used, these results suggest a reformulation in the selection indexes of the beef production system in tropical and subtropical regions in order to obtain greater profitability. 相似文献