全文获取类型
收费全文 | 26932篇 |
免费 | 837篇 |
国内免费 | 35篇 |
专业分类
林业 | 4502篇 |
农学 | 1637篇 |
基础科学 | 166篇 |
5143篇 | |
综合类 | 1494篇 |
农作物 | 2524篇 |
水产渔业 | 2521篇 |
畜牧兽医 | 5690篇 |
园艺 | 1301篇 |
植物保护 | 2826篇 |
出版年
2023年 | 101篇 |
2022年 | 127篇 |
2021年 | 236篇 |
2020年 | 227篇 |
2019年 | 153篇 |
2018年 | 3056篇 |
2017年 | 3004篇 |
2016年 | 1579篇 |
2015年 | 440篇 |
2014年 | 452篇 |
2013年 | 681篇 |
2012年 | 1625篇 |
2011年 | 2809篇 |
2010年 | 2428篇 |
2009年 | 1528篇 |
2008年 | 1953篇 |
2007年 | 2171篇 |
2006年 | 579篇 |
2005年 | 666篇 |
2004年 | 624篇 |
2003年 | 638篇 |
2002年 | 468篇 |
2001年 | 311篇 |
2000年 | 296篇 |
1999年 | 229篇 |
1998年 | 77篇 |
1997年 | 60篇 |
1996年 | 43篇 |
1995年 | 71篇 |
1994年 | 41篇 |
1993年 | 47篇 |
1992年 | 89篇 |
1991年 | 69篇 |
1990年 | 64篇 |
1989年 | 63篇 |
1988年 | 68篇 |
1987年 | 54篇 |
1986年 | 49篇 |
1985年 | 47篇 |
1984年 | 42篇 |
1983年 | 34篇 |
1979年 | 37篇 |
1978年 | 23篇 |
1974年 | 27篇 |
1973年 | 22篇 |
1972年 | 29篇 |
1971年 | 24篇 |
1970年 | 29篇 |
1969年 | 28篇 |
1968年 | 29篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
111.
Reto M Figueira ME Filipe HM Almeida CM 《Plant foods for human nutrition (Dordrecht, Netherlands)》2007,62(4):139-144
To evaluate the potential benefits and risks associated with tea consumption it is important to identify the constituents
of this beverage. Levels of some minerals, caffeine and catechins in green tea samples commercialized in Portugal were evaluated.
Potassium is the metal present in larger amount (92–151 mg/l). The content of sodium, calcium, fluoride, aluminium, manganese
and iron were 35–69, 1.9–3.5, 0.80–2.0, 1.0–2.2, 0.52–1.9, 0.020–0.128 mg/l, respectively. Chromium and selenium were not
detected. The resulting data showed considerable variability in catechins content. The levels of epigallocatechin gallate
(EGCG) ranged from 117 to 442 mg/l, epicatechin 3-gallate (EGC) from 203 to 471 mg/l, epigallocatechin (ECG) from 16.9 to
150 mg/l, epicatechin (EC) from 25 to 81 mg/l and catechin (C) from 9.03 to 115 mg/l. Caffeine contents in the green tea infusions
studied were between 141–338 mg/l. Green tea infusions provide significant amounts of catechins and could be an important
source of some minerals. 相似文献
112.
Growth,P uptake and rhizosphere properties of wheat and canola genotypes in an alkaline soil with low P availability 总被引:1,自引:0,他引:1
Zakaria Solaiman Petra Marschner Dongmei Wang Zed Rengel 《Biology and Fertility of Soils》2007,44(1):143-153
The aim of the present study was to assess the role of soil type on growth, P uptake and rhizosphere properties of wheat and
canola genotypes in an alkaline soil with low P availability. Two wheat (Goldmark and Janz) and two canola genotypes (Drum
and Outback) were grown in a calcareous soil (pH 8.5) at two P levels [no P addition (0P) or addition of 200 mg kg−1 P as Ca3(PO4)2 (200P)] and harvested at flowering or maturity. Shoot and root dry weight, root length and shoot P content were greater in
the two canola genotypes than in wheat. There were no consistent differences in available P, microbial P and phosphatase activity
in the rhizosphere of the different genotypes. Shoot P content was significantly positively correlated with root length, pH
and phosphatase activity in the rhizosphere. The microbial community composition, assessed by fatty acid methylester analysis,
of the canola genotypes differed strongly from that of the wheat genotypes. The weight percentage bacterial fatty acids, the
bacteria/fungi (b/f) ratio and the diversity of fatty acids were greater in the rhizosphere of the canolas than in the rhizosphere of the wheat
genotypes. In contrast to the earlier studies in an acidic soil, only small differences in growth and P uptake between the
genotypes of one crop were detected in the alkaline soil used here. The results confirmed the importance of root length for
P uptake in soils with low P availability and suggest that the rhizosphere microbial community composition may play a role
in the better growth of the canola compared to the wheat genotypes. 相似文献
113.
114.
Rosangela Simeão Adriane Silva Cacilda Valle Marcos Deon Resende Sérgio Medeiros 《Plant Breeding》2016,135(2):246-253
Half‐sib progenies of congo signal grass (Brachiaria ruziziensis Germain & Evrard, syn.: B. eminii, Urochloa ruziziensis) were analysed using a mixed model methodology. The objective was to estimate genetic and phenotypic parameters, predict individual genetic values, estimate correlations between characters associated with biomass production and forage nutritive value and use this information to determine the economic weights to compare three multiplicative selection indices. Individual narrow‐sense heritabilities corrected for inbreeding varied from 0.14 to 0.91 for characters associated with biomass production and from 0.04 a 0.24 for nutritional value characters. The correlations among characters associated with annual biomass production were of high magnitude. In contrast, biomass production characters were negatively correlated with crude protein, acid detergent fibre and lignin. Total dry matter yield, crude protein and regrowth ability were weighted separately in evaluations during wet and dry season or annually in selection indices. Index that considered characters annually resulted in better distribution of forage production along the year than indices that separated wet and dry season production. 相似文献
115.
Edak Aniedi Uyoh Ikootobong Sunday Urua Valentine Otang Ntui Elza Cletus Okpako 《Journal of Crop Science and Biotechnology》2011,14(4):227-232
Nuclear DNA of three accessions of Parkia biglobosa collected from three locations in northern Cross River State was investigated using a Patec PA II flow cytometer equipped
with an argon ion laser (488 nm), and pictures of mitotic chromosomes were taken using a digital micro-camera (Canon) placed
on the eye piece of a binocular microscope at 100X oil immersion. Metaphase chromosome counts of 2n = 22 for accessions A
and C and 2n = 24 for accession B, were obtained and through flow cytometry, the three accessions were confirmed to be diploids.
The nuclear DNA content and genome size for the accessions were 1.5085, 1.489, and 1.5266 pg (737.7054, 728.121, and 746.5074
Mbp) for accessions A, B, and C, respectively. In another experiment, leaf samples from greenhouse-germinated seeds were analyzed
for variation in the banding pattern of the protein by SDS-PAGE in the three accessions. Protein was resolved into three banding
groups according to their electrophoretic mobility: slow, medium, and fast, clustering between 100–200, 40–70, and 10–25 kDa,
respectively. There was 76% similarity in the banding pattern between the accessions. 相似文献
116.
Canopy cover (CC) is a good predictor variable for plant growth parameters such as leaf area index and aboveground biomass.
A nondestructive, low-cost, and convenient method is presented for estimating CC using digital camera image analysis. CC was
estimated by the ratio of plant pixels to total pixels of digital camera image of rice field. To determine the criteria for
segmenting the rice plant from variable soil background, three mosaic images for rice plant, flooded/bare soil, and algae-infested
background were prepared from digital camera images that were taken in various field conditions. An image analysis program
was developed in Visual Basic to extract red, green, and blue (RGB) features from the mosaic images, calculate RGB-based color
indices, and compute the minimum segmentation error for separating rice plant from background. When judged by the segmentation
error, modified excessive green index (MEGI) showed the highest potential for segmenting rice plant from flooded/bare soil
background, followed by normalized green (g) and excessive green index (EGI). At the threshold MEGI value of 0.03, the segmentation
error was the lowest as 0.13%. Any single index considered was not satisfactory in segmenting rice plant from algae-infested
background. However, a discriminant function of 1.2553EGI + 0.01735G − 0.01474B was successful in segmenting rice plant from
flooded/bare soil and algaeinfested background with segmentation errors of 0.34 and 1.17%, respectively. CC for four rice
varieties from tillering to booting stage was estimated based on the threshold value of MEGI and discriminant function and
also manually using commercial software. Both estimates of CC showed good relationship of r2 = 0.94, suggesting that a digital camera could be used efficiently for measuring the CC of rice field. 相似文献
117.
S.J. Ochatt A. Benabdelmouna P. Marget G. Aubert F. Moussy C. Pontécaille L. Jacas 《Euphytica》2004,137(3):353-359
Pea would benefit from the plasticity and adaptability of its cross-incompatible relatives Pisum fulvum and Lathyrus sativus
L., and we have tested reciprocal sexual crossings by manually cross-pollinating plants of genotypes of these three species. Studies of in situ germination of pollen grains on stigmata showed that pollen tubes were generally unable to germinate or could not reach the ovary. A few putative hybrid pods were nevertheless harvested, with one grain per pod germinated in vitro, then micropropagated for flow cytometry, isoenzyme, molecular (ribosomal ITS PCR-RFLP) and genomic in situ hybridization (GISH) studies. One such grain was recovered from an inter-generic cross of P. sativum x L. sativus and four from an inter-specific P. sativum x P. fulvumcross. A strong cross-incompatibility was shown between pea and grass pea, where the putative hybrid turned out to be pea. Conversely, with the interspecific, P. sativum x P. fulvum cross, flow cytometry and isoenzymes with leaf tissues strongly suggested hybridity, while molecular approaches and GISH confirmed the production of inter-specific hybrids, and without the need for a wild type P. sativum accession as a bridging cross. 相似文献
118.
Philippe Cubry Valérie Pujade‐Renaud Dominique Garcia Sandra Espeout Vincent Le Guen Françoise Granet Marc Seguin 《Plant Breeding》2014,133(3):419-426
Despite its economic importance and recent genome release, the need for molecular tools for Hevea brasiliensis is high. In the frame of a disease resistance study, EST sequences were retrieved from public database or generated by sequencing SSH libraries. Sequences were trimmed and microsatellite motifs searched using an ad hoc bioinformatic pipeline, and pairs of primers for the amplification of candidate markers were generated. We found a total of 10 499 unigenes from both sources of sequences, and 673 microsatellites motifs were detected using the default parameters of the pipeline. Two hundred sixty‐four primer pairs were tested and 226 (85.6%) successfully amplified. Out of the amplified candidate markers, 164 exhibited polymorphism. Relationships based on dendrograms using simple matching index and diversity statistics based on EST‐SSRs were compared with Genomic SSRs, showing the potentialities of EST‐derived microsatellites for resistance studies but also for population genetics approaches. 相似文献
119.
120.
为了明确杨梅疏果核仁中苦杏仁苷的提取方法,采用丁岙种杨梅的疏果为原料,以苦杏仁苷提取率为响应值,在超声波提取单因素试验和二水平试验的基础上,选择乙醇浓度、料液比、提取温度作为试验因子,利用Design Expert 80软件进行三因素三水平的响应面试验设计,并建立二次回归方程的预测模型。试验确定了杨梅疏果核仁中苦杏仁苷的最佳提取工艺条件: 乙醇浓度27%,料液比1∶45 (m/V),提取时间20 min,提取温度44℃,超声功率250 W。在该条件下苦杏仁苷理论提取率为9403%,实际提取率为9380%,与模型预测值基本相符。 相似文献