Identification of cultivars of the forage legume Pueraria phaseoloides by electrophoretic patterns of storage proteins

Summary A polyacrylamide gel electrophoresis procedure was developed for discriminating cultivars of the forage legume Pueraria phaseoloides on the basis of the patterns of cotyledon proteins. The genotypic marker proteins were extracted with 5M acetic acid and electrophoresed at pH 3.1 in aluminum lactate buffer. The procedure gave highly reproducible discrimination of ten selected cultivars.     

Bovine uterine defense mechanisms: a review

Bovine uterine defense mechanisms during physiological and pathological conditions have been reviewed in this article. The initial uterine defense against bacterial infection is phagocytosis by uterine leucocytes (mainly neutrophils). The reported literature showed that very little work has been done on immunoglobulins and their role in the bovine uterine defense mechanisms; however, some investigators have found a positive correlation between gamma-globulin and the development of uterine infection after calving. Many explanations exist for the difference in susceptibility of the uterus to infection during the different phases of estrous cycle; however, most of the reports agreed that the uterine defense mechanism is inadequate during diestrus. The abnormal puerperium effects uterine defense mechanisms adversely and prolongs the time to complete uterine involution. Future treatment may utilise natural antimicrobial substances such as proteins or peptides derived from PMN, chemoattractant substances such as E. coli lipopolysaccharide or a bacteria-free filtrate of streptococci. Specific hyperimmunserum could also be used as opsonin for refractory cases of uterine bacterial infections.     

Corynebacterium pseudotuberculosis infection and lymphadenitis (taloa or mala) in the camel

Summary Pure cultures ofCorynebacterium pseudotuberculosis were obtained from 11 cases of lymphadenitis (known locally as taloa or mala) in camels. Camel isolates produced typical taloa in camels experimentally inoculated subcutaneously at the base of the external ear with 10¹⁰ colony forming units. A sheep strain ofC. pseudotuberculosis inoculated into camels produced a local abscess at the site of inoculation but did not produce taloa. Re-infection of camels recovered from experimental inoculation did not produce taloa suggesting the possibility of the development of a vaccine against lymphadenitis in camels.

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Infeccion PorCorynebacterium Pseudotuberculosis Y Linfadenitis (Taloa O Mala) En El Camello

Resumen Se obtuvieron cultivos puros deCorynebacterium pseudotuberculosis a partir de 11 casos de linfadenitis (conocida localmente como taloa o mala) en camellos. La inoculación subcutánea en camellos en la base de la oreja de 10¹⁰ unidades formadoras de colonias procedentes de un cultivo obtenido de camellos infectados causó taola. Sin embargo, una cepa ovina deC. pseudotuberculosis causó un abceso en el lugar de la inoculación pero no produjo taola en camellos. La reinfección de los camellos que se habían recuperado de la inoculación experimental no causó taola, lo que sugiere la posibilidad de desarrollar una vacuna frente a la linfadenitis caseosa del camello.

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Infection ACorynebacterium Pseudotuberculosis Et Lymphadenite (Taloa Ou Mala) Chez Le Chameau

Résumé On a obtenu des cultures pures deCorynebacterium pseudotuberculosis à partir de 11 cas de lymphadénite (dénommée localement taloa ou mala) chez des chameaux. L'inoculation expérimentale des isolements camelins faite en infection sous-cutanée à la base de l'oreille externe avec 10¹⁰ unités formant des colonies a provoqué un taloa typique. Une souche ovine deC. pseudotuberculosis inoculée à des chameaux n'a conduit qu'à la formation d'un abcès local au point d'inoculation mais pas au taloa. La réinfection des chameaux guéris d'une inoculation expérimentale ne provoque pas de taloa, ce qui permet d'augurer la possibilité de développement d'un vaccin contre la lymphadénite cameline.

     

Glycine betaine counteracts the inhibitory effects of waterlogging on growth,photosynthetic pigments,oxidative defence system,nutrient composition,and fruit quality in tomato

Glycine betaine (GB) is an important organic compound mediating plant responses to environmental stresses. However, despite ample research on this biomolecule, the potential of GB in mitigating the effects of waterlogging in plants has not been established. Therefore, we studied the influence of GB on growth and physiology of tomato plants under waterlogged conditions. Waterlogging reduced plant growth, degraded chlorophyll, and increased concentration of malondialdehyde and hydrogen peroxide that deteriorated membrane integrity. Waterlogging increased catalase and peroxidase activities. Waterlogging increased the concentration of Na and reduced concentrations of K. Reductions in root Ca were also recorded. GB enhanced growth, concentration of chlorophyll and activities of superoxide dismutase, catalase, and peroxidase that in turn protected the plants from oxidative damage. GB decreased Na while increasing leaf and root K and stem and fruit Ca under waterlogging. Waterlogging reduced fruit quality. There was decrease in protein, fat, and total dissolved solids and an increase in fruit moisture, P, and Na in plants under waterlogging. GB enhanced fruit quality largely by improving fruit protein, ash, fat, TDS, and Ca, while it decreased fruit Na. The results of this study suggest the use of GB for commercial production of tomato where waterlogging is likely.     

Determination of prevalence,serological diversity,and virulence of Dichelobacter nodosus in ovine footrot with identification of its predominant serotype as a potential vaccine candidate in J&K,India

The aim of this study was to determine the prevalence, serological diversity, and virulence of Dichelobacter nodosus in footrot lesions of sheep and identification of its predominant serotype as a potential vaccine candidate. The overall prevalence of footrot in sheep was 16.19%, and ranged from 13.69 to 19.71%, respectively. A total of 759 flocks with 22,698 sheep were investigated for footrot and 2374 clinical samples were collected from naturally infected sheep exhibiting footrot lesions. Of the 2374 samples collected, 1446 (60.90%) were positive for D. nodosus by polymerase chain reaction (PCR). These positive samples when subjected to serogroup-specific multiplex PCR, 1337 (92.46%) samples carried serogroup B, 247 (17.08%) possessed serogroup E, 86 (5.94%) serogroup I, and one (0.069%) serogroup G of D. nodosus. While mixed infection of serogroups B and E was detected in 127 (8.78%), B and I in 46 (3.18%) and B, E, and I in 26 (1.79%) samples, respectively. The serogroup B of D. nodosus was the predominant (92.47%) serogroup affecting sheep population with footrot followed by serogroup E (19.91%) and serogroup I (4.57%), respectively. Virulent status of D. nodosus strains were confirmed by presence of virulence-specific integrase A (intA) gene and the production of thermostable proteases. The intA gene was detected in 709 (72.79%) samples while gelatin gel test carried out on 246 representative isolates all positive for intA gene produced thermostable proteases, confirming their virulence nature. The PCR-restriction fragment length polymorphism (PCR-RFLP) of whole fimA gene of serogroup B revealed the predominance of serotype B5 (82.97%) of serogroup B. This information suggests that serotype B5 is the predominant serotype of D. nodosus associated with severe footrot lesions in sheep in Jammu & Kashmir (J&K), India. Hence, this serotype can be a potential vaccine candidate for the effective control and treatment of ovine footrot.

     

Zinc Ameliorates Fruit Yield and Quality of Mangoes Cultivated in Calcareous Soils

Zinc (Zn) deficiency is frequently observed in mangoes grown in calcareous soils. Inherent inability of mango to extract sufficient Zn from the soil exacerbates this situation. Therefore, mitigating effects of Zn application was investigated on flowering, fruit setting, yield and quality of mango cv. Samar Bahisht Chaunsa growing in calcareous soils. The experiment consisted of five treatments: control—no Zn, soil application of 50 and 100 g ZnSO₄ per tree and foliar application of 0.5? and 1.0?% ZnSO₄ solution. Zn was applied twice in the 2^nd week of November, 2011 and 3^rd week of March, 2012, respectively. The experiment was repeated on the same dates during the next growing season. Zn application significantly increased number of flower panicle, fruits matured panicle, leaf Zn concentrations, fruit size, peel thickness, fruit weights at harvest and after ripening and fruit yield tree, whereas it reduced malformed panicles and early fruit drop. However, flower sex ratio and fruit set remained statistically unaffected. Similarly, fruit quality in terms of total soluble solids, flesh color, aroma, flavor and overall acceptability significantly improved as compared to control. Foliar application mitigated Zn deficiency more effectively than the soil application in calcareous soils.     

Utilisation of leucaena leaf meal by broilers

1. The effect of including Leucaena (Leucaena leucocephala) leaf meal (LLM) in broiler diets was investigated employing LLM at 0, 50, 100, 150 and 200 g/kg diet. Experiments were also conducted to investigate whether reduced food intake of diets containing LLM is responsible for the growth depression.

2. LLM appears to be a good source of protein and calcium.

3. Food intake was not significantly affected (P>0.05) with dietary LLM inclusion up to 200 g/kg.

4. Inclusion of LLM up to 150 g/kg diet did not influence significantly (P>0.05) the performance of broilers in terms of body weight gain and food efficiency. However, inclusion of LLM at 200 g/kg diet had an adverse effect on weight gain and food efficiency, when compared to other dietary treatments. The profitability over food costs was low with the 200 g LLM/kg diet compared to those of other diets.

5. It may be inferred from these experiments that LLM can safely be included in broiler diets up to 150 g/kg.

6. Studies using force‐feeding and restricted feeding techniques indicated that reduced food intake was not responsible for the growth depression in diets containing higher amounts of LLM.     

Bacillus subtilis B21 and Bacillus licheniformis B26 improve intestinal health and performance of broiler chickens with Clostridium perfringens‐induced necrotic enteritis

This study investigated the influence of Bacillus‐based probiotics on performance and intestinal health in broiler challenged with Clostridium perfringens‐induced necrotic enteritis. One‐day‐old Arbor Acre (n = 480) were randomly assigned to four treatments with 10 cages of 12 birds: (a) basal diet negative control (NC), with no probiotics nor antibiotics formulated to contain 2,930 and 3,060 kcal/kg with 24.07 and 15.98% CP, for starter and finisher diet, respectively, (b) basal diet + enramycin (5 mg/kg), an antibiotic growth promoter (AGP); (c) basal diet + Bacillus subtilis B21 at 2 × 10⁹ CFU per g (BS); (d) basal diet + Bacillus licheniformis B26 at 2 × 10⁹ CFU per g (BL); growth performance, intestinal morphology, intestinal lesion scores, short‐chain fatty acids (SCFAs) and mucosal barrier tight junction's (TJ) mRNA expression were assessed. NC‐ and BL‐fed groups showed higher (p = 0.005) average daily feed intake from d1 to d21 than AGP and BS, whereas BS‐ and AGP‐fed groups showed higher average daily weight gain from d22 to d42 and d1 to d42 of age. Higher mortality rate of (12.5%) and lower of (5.5%) were recorded in AGP and NC fed‐groups respectively, lesion score was higher in BS and BL than in AGP, while no lesion was observed in NC group, results revealed higher duodenum and jejunum villus height to crypt depth (VH:CD) compared with NC and BS. Probiotics‐fed groups showed higher total (SCFAs), acetic and butyric acid concentrations at d21 post‐challenge (PC) than other groups. The expression of claudin‐1 was upregulated in duodenum (d7) PC and in jejunum (d7) and (d21) PC in BL group, while at d21 PC, the expression of occludens was higher in jejunum and ileum by AGP and BL. The present study indicated both BS and BL have some similarity with AGP in preventing or partially preventing NE effect in broilers.     

Concurrent vaccination of goats with foot and mouth disease (FMD) and peste des petits ruminants (PPR) booster vaccines

Foot and mouth disease (FMD) remains subclinical and self-limiting in small ruminants, but risk of spread of infection to susceptible cohorts is of great epidemiological significance; therefore, small ruminants must be included in vaccination campaigns in FMD endemic regions. Three groups of goats already immunized against peste des petits ruminants (PPR) were vaccinated with FMD and PPR vaccines alone or concurrently. The specific antibody response against three FMD virus strains and PPR virus were evaluated by competitive enzyme-linked immunosorbent assay (cELISA). Goats concurrently vaccinated with PPR + FMD vaccines had significantly (p < 0.05) higher antibody titers to two serotypes of FMD virus at 28, 45, and 60 days post-immunization compared to goats vaccinated with FMD vaccine alone, while goats vaccinated with PPR vaccines alone or PPR + FMD vaccines concurrently showed similar antibody kinetics against PPR virus up till 60 days post-vaccination. Overall, antibody kinetic curves for all three tested strains of FMD virus and PPR virus were similar in vaccinated groups during the course of experiment.     

Mini review on photosensitization by plants in grazing herbivores

Photosensitization is severe dermatitis or oxidative/chemical changes in the epidermal tissues activated by the light-induced excitation of molecules within the tissue. It is a series of reactions mediated through light receptors and is more common when the plant-produced metabolites are heterocyclic/polyphenols in nature. The areas affected are exposed body parts and mostly non-pigmented areas with least ultraviolet protection. Similarly, cellular alteration also occurs in the affected animal’s dermal tissues and body parts and grazing animals by the accumulation and activation of photodynamic molecules. Photo-oxidation can also occur within the plant due to the generation of reactive oxygen species causing damage and degradation in the form of free radicals and DNA. During the last few decades, many new tropical grass species have been introduced in the grazing lands which are genetically modified, and the animals grazing on them are facing various forms of toxicity including photosensitization. The plant’s secondary metabolites/drugs may cause toxicity when bacteria, viral agents, fungi (Pithomyces chartarum), or neoplasia injures the liver and prevents the phylloerythrin excretion. All these may disturb the liver enzymes and blood profile causing a decrease in weight and production (wool and milk etc.) with severe dermal, digestive, and nervous problems. Recent advancements in OMICS (cellomics, ethomics, metabolomics, metabonomics, and glycomics) have enabled us to detect and identify the plants’ secondary metabolites and changes in the animal’s physiology and histopathology as a causative of photosensitivity. The review focuses on types of photosensitization, reasons, secondary metabolic compounds, chemistry, and environmental effect on plants.