GRASSLAND RESEARCH ON BLANKET PEAT IN IRELAND

The results from 10 years of grassland research at the Peatland Experimental Station, Glenamoy, are summarized.
Grass and legume species were introduced by surface seeding, without cultivation or by direct seeding after rotavation, depending on the specific situation. The rate of establishment was dependent on pretreatment and the management of grazing animals before and after sowing.
The most suitable species were Trifolium repens, Lolium perenne, Festuca arumlinacea, Festuca rubra and Holcus lanatus .
A level of fertility suited to the introduced species must be reached initially and subsequently maintained. The requirements (per/ac) for Ca, P, K, N, Cu and Co were: 2240 lb (1016 kg) ground limestone, 36 lb (16 kg) P, 112 lb (51 kg) K, 20 lb (9 kg) N, 20 lb (9 kg) CuSO₄ and 2 lb (0–91 kg) CoSO₄ at sowing, with 27 lb (12–25 kg) P and 56 lb (254 kg) K annually.
Dry–matter yields of 6000 lb/ac (6720 kg/ha) were obtained from grass–clover swards without fertilizer N, and approximately twice this quantity where 400 lb N/ac (448 kg/ha) had been applied annually. A liveweight gain of 526 lb/ac (589–12 kg/ha) and a starch equivalent output of 2946 lb (3299–5 kg) were obtained with sheep. The relationship between these results and those on peat soils abroad are discussed.     

Improved seed development and germination of stenospermic grapes by plant growth regulators

Plant growth regulators were applied to the foliage and immature fruit clusters of the stenospermic grape selection ‘C35-33’ at various periods before bloom to stimulate viable seed development. In the 1987 season five different plant growth regulators were used, but in 1988 the growth retardants Cycocel and XE-1019 were used exclusively. Chemical treatments applied 35 days after bud break increased significantly germination percentage. Experimental results indicate that the use of certain plant growth regulators may aid in increasing the efficiency of seedless grape breeding by providing an alternative to in-ovulo embryo culture.     

The effects of mechanically-induced stress and water stress on freezing resistance in lettuce and cauliflower seedlings

Summary

Mechanically-induced stress, applied by brushing young lettuce and cauliflower plants for 90 s each day, reduced the freezing resistance of cauliflower but had no effect on that of lettuce. Brushing reduced fresh weight in both species and smaller plants were less freezing-resistant than larger ones. The levels of abscisic acid (ABA) in the shoots of brushed cauliflowers were slightly less than in unbrushed plants, whereas in lettuce the levels were similar in the two treatments. ABA sprayed onto lettuce plants had no effect on freezing resistance. With both lettuce and cauliflower, freezing resistance, and the osmolarity of sap extracted from the shoots, increased following water-stress and declined progressively following rehydration.     

Modifications of wheat flour proteins during in vitro digestion of bread dough, crumb, and crust: an electrophoretic and immunological study

The proteins of wheat flour have several biological activities that can affect human health and physiology when wheat-based foods are consumed. The modifications of bread crumb and crust proteins during an in vitro peptic/pancreatic digestion process were studied by electrophoresis and immunoblotting with polyclonal antibodies specific for single proteins or groups of homologous proteins of the wheat flour, and the results were compared to those obtained for an unheated dough sample. The results show that baking affects the extent of proteolysis and the immunological and physicochemical features of the digestion products in relation to the level of the heat treatment. Therefore, the results concerning the digestion of the unheated wheat flour or dough are not representative of what happens when baked products enter the human digestive tract.     

Determination and toxicity of saponins from Amaranthus cruentus seeds.

The concentrations of four triterpene saponins present in amaranth seeds were determined with high-performance liquid chromatography. It was shown that the total concentration of saponins in seeds was 0. 09-0.1% of dry matter. In germinating seeds an increase in concentration to 0.18% was observed after 4 days of germination, which remained stable for the next 3 days and later dropped to 0.09%. Highly purified extracts from the seeds were tested for their toxicity against hamsters. The hydrophobic fraction obtained by the extraction of seeds with methylene chloride showed no toxicity; the behavior of tested animals was similar to that of the group given an equivalent dose of rapeseed oil. A crude saponin fraction, containing approximately 70% of pure saponins in the matrix, showed some toxicity; the approximate lethal dose was calculated as 1100 mg/kg of body weight. It is concluded that low contents of saponins in amaranth seeds and their relatively low toxicity guarantee that amaranth-derived products create no significant hazard for the consumer.     

Expression of genes involved in the early immune response at the distal segment of the gut in Atlantic cod, Gadus morhua L. after vaccination with a bacterial antigen

The gastro-intestinal tract serves as the route of infection of most bacterial pathogens in fish, and the immune response genes in the gut produce substances that provide an initial defense during pathogen invasion. The expression of some genes involved in antibacterial defense, cell-mediated immunity and inflammation in the distal intestine of Atlantic cod, Gadus morhua L., was investigated following intraperitoneal vaccination with heat-killed Vibrio anguillarum. Tissue samples of the distal intestine were collected at 0, 1, 3, 7 and 10 days post-vaccination (dpv) and processed for semi-quantitative RT-PCR analyses. Expression of the bactericidal/permeability-increasing protein/lipopolysaccharide-binding protein (BPI/LBP), g-type lysozyme, non-specific cytotoxic cell receptor protein-1 (NCCRP-1) and interleukin-8 (IL-8) significantly increased during the early stages post-vaccination, that is, 1–3 dpv, followed by a decrease in their expression returning to their initial levels. Transferrin and apolipoprotein A-I, although weakly expressed in the distal intestine, also increased in the vaccinated fish. Interleukin-1β (IL-1β) was significantly decreased in its expression level relative to the PBS-injected fish at 3 and 7 dpv. The expression of these immune-related genes in the distal intestine as a result of vaccination provides valuable information on the mechanisms of gut immunity in the fish against pathogenic bacteria, particularly during the early stages of infection.     

EVALUATION OF POTENTIAL SUBSTRATES TO MONITOR RESPIRATORY NITRATE REDUCTASE ACTIVITY IN SOILS

Different inorganic ions were tested for their ability to replace nitrate as the terminal oxidant for bacterial respiration in anaerobic soils. Chlorate, bromate. selenate. tellurite and ferric ions were all unsuitable substitutes, lodate reduction in soils was similar to nitrate reduction because it required anaerobic conditions, was increased by glucose amendment and decreased by phenylmercuric acetate. Amendment of soils with iodate and measurement of iodide production can be used to measure nitrate reductase activity in a soil and so give a qualitative indication, and possibly a quantitative estimate, of the denitrification capacity of soils.