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排序方式: 共有466条查询结果,搜索用时 31 毫秒
31.
Kobayashi N Fujiwara T Westerman KA Inoue Y Sakaguchi M Noguchi H Miyazaki M Cai J Tanaka N Fox IJ Leboulch P 《Science (New York, N.Y.)》2000,287(5456):1258-1262
Because of a critical shortage in suitable organs, many patients with terminal liver disease die each year before liver transplantation can be performed. Transplantation of isolated hepatocytes has been proposed for the temporary metabolic support of patients awaiting liver transplantation or spontaneous reversion of their liver disease. A major limitation of this form of therapy is the present inability to isolate an adequate number of transplantable hepatocytes. A highly differentiated cell line, NKNT-3, was generated by retroviral transfer in normal primary adult human hepatocytes of an immortalizing gene that can be subsequently and completely excised by Cre/Lox site-specific recombination. When transplanted into the spleen of rats under transient immunosuppression, reversibly immortalized NKNT-3 cells provided life-saving metabolic support during acute liver failure induced by 90% hepatectomy. 相似文献
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Thermodynamic data are presented for hydrates of nitric acid: HNO(3).H(2)O, HNO(3).2H(2)O, HNO(3).3H(2)O, and a higher hydrate. Laboratory data indicate that nucleation and persistence of metastable HNO(3).2H(2)O may be favored in polar stratospheric clouds over the slightly more stable HNO(3).3H(2)O. Atmospheric observations indicate that some polar stratospheric clouds may be composed of HNO(3).2H(2)O and HNO(3).3H(2)O. Vapor transfer from HNO(3).2H(2)O to HNO(3).3H(2)O could be a key step in the sedimentation of HNO(3), which plays an important role in the depletion of polar ozone. 相似文献
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Tail removal from dominant juvenile Uta stansburiana resulted in a decrease in social status in dyadic encounters. Most lizards were affected after removal of two-thirds of their tails. In some pairs, dominant lizards lost status after removal of one-third of their tails but regained dominance after the other member of the pair lost two-thirds of its tail. Tail loss in nature may impose a social handicap to successful home range acquisition and thereby increase risk of death. 相似文献
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Fox P 《Science (New York, N.Y.)》1913,37(940):25-40
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Kolesnikova OA Entelis NS Mireau H Fox TD Martin RP Tarassov IA 《Science (New York, N.Y.)》2000,289(5486):1931-1933
Mitochondrial import of a cytoplasmic transfer RNA (tRNA) in yeast requires the preprotein import machinery and cytosolic factors. We investigated whether the tRNA import pathway can be used to correct respiratory deficiencies due to mutations in the mitochondrial DNA and whether this system can be transferred into human cells. We show that cytoplasmic tRNAs with altered aminoacylation identity can be specifically targeted to the mitochondria and participate in mitochondrial translation. We also show that human mitochondria, which do not normally import tRNAs, are able to internalize yeast tRNA derivatives in vitro and that this import requires an essential yeast import factor. 相似文献
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