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71.
Petra Persson Kristina Sundell Björn Th Björnsson 《Fish physiology and biochemistry》1994,13(5):379-386
Juvenile rainbow trout, Oncorhynchus mykiss, were injected with estradiol-17β (E2) in order to study the source of extra calcium needed during vitellogenesis. E2-treatment increased the calcium uptake from the external medium as well as calcium mobilization from muscle and scale. Judged
by the increase in plasma protein-bound calcium levels, the E2-induced increase in calcium uptake is an apparent over-mobilization of calcium, i.e., the calcium uptake of the fish is in excess of what is found bound to plasma proteins. As the calcium excretion and calcium
space (calculated from free plasma calcium levels) were unaffected, the excess calcium is suggested to be incorporated into
internal calcium stores. This implies that the systems regulating vitellogenesis and calcium balance are integrated on the
mechanistic or endocrine level, and that E2 causes calcium mobilization of a magnitude geared to the needs of the sexually maturing female. 相似文献
72.
Susana?BenedetEmail author Viktoria?Johansson Glen?Sweeney Malyka?Galay-Burgos Bj?rn?Thrandur?Bj?rnsson 《Fish physiology and biochemistry》2005,31(4):315-329
Two isoforms of the full-length cDNA of the growth hormone receptor (GHR) of the Atlantic salmon (Salmo salar; ss) were cloned by a PCR approach using RACE. Respectively, the cDNA sequences of ssGHR isoforms 1 and 2 are 2654 and 2608 nucleotides long, with 1782 and 1773 nucleotide ORFs. The resulting coded proteins
are 594 and 590 aa long, with 19 and 20 aa signal peptides. The two isoforms share 86% protein and 87% cDNA sequence similarity.
Isoform 1 is most similar to other salmonid GHR isoforms 1 while isoform 2 is most similar to salmonid GHR isoforms 2 (93–95%).
Similarity with other teleost species was lower (37–44%). The bioactivity of the cloned ssGHR was tested by transfecting the ssGHR isoform 1 cDNA into CHO-K1 hamster cells, incubating with recombinant salmon GH (sGH) or native ovine prolactin (oPRL),
and measuring cell proliferation by the MTT assay. The ssGHR-transfected cells significantly increased proliferation when stimulated by sGH at all concentrations. oPRL stimulated
ssGHR-transfected cells at higher concentrations due to receptor cross reaction. ssGHR isoforms 1 and 2 contain a single transmembrane domain and the typical conserved motifs found in other teleost GHRs, including
four paired cysteine residues and five potential N-glycosylation sites in the extracellular domain, Box I and Box II, as well as seven potential tyrosine phosphorylation sites
in the intracellular domain. However, in salmonids, these motifs differ from those of other teleosts, and could be responsible
for differentiated hormone binding, signal transduction and response. 相似文献
73.
Karlsson A Heier LS Rosseland BO Salbu B Kiessling A 《Fish physiology and biochemistry》2011,37(1):249-258
Free-swimming Atlantic cod (Gadus morhua) were exposed to water oxygen pressures (P
wO2) ranging from 18.1 to 41.5 kPa and sampled for blood using an indwelling caudal artery cannula. Arterial blood oxygen pressure
(P
aO2) increased with increasing P
wO2, from 12.0 kPa in normoxia (18.1 kPa) to 34.2 kPa in the highest hyperoxic level tested (41.5 kPa). Blood CO2 pressure and plasma bicarbonate concentration increased with P
wO2, indicating reduced ventilation with increased P
wO2. Plasma glucose, sodium and potassium were not affected by water oxygen level. Blood oxidative stress biomarkers, reduced
glutathione, oxidized glutathione and the oxidative stress index (ratio between oxidized and total glutathione) differed intermittently
between normoxia and hyperoxia. The oxidative stress index was higher in the blood of exposed compared to unexposed control
cod. Together with elevated P
aO2, these findings suggest increased production of reactive oxygen species and increased oxidative stress in Atlantic cod exposed
to hyperoxia. 相似文献
74.
Lushani Nanayakkara Elizabeth R. Starks Ryan N. Cooper Sydney Chow Peter R. Leavitt Björn Wissel 《Fisheries Management and Ecology》2021,28(1):18-27
This study evaluated the status of native and stocked fish species in 13 prairie lakes in central Canada over eight years (2007–14) using three metrics: resource-use (benthic versus pelagic carbon via stable isotopes); body condition (relative weight index Wr); and parasite load (cestode gut enumerations). Analyses included game and non-game fishes, like naturally occurring northern pike, Esox lucius L., and yellow perch, Perca flavescens Mitchill, but focused on stocked walleye, Sander vitreus (Mitchill) because it supports a robust recreational fishery. Walleye and northern pike were significantly more reliant on benthic carbon than yellow perch or forage fishes (p < 0.05), but this reliance was not associated with any measured environmental variables for any species. In lakes with game fishes, forage fishes exhibited higher reliance on benthic energy, possibly due to predator avoidance strategy, particularly yellow perch. Walleye body condition index was consistently lower (<95–105) than values exhibited by the other two game fishes (81–139), and parasites were only common in walleye and associated with lake-water salinity (r2 = 0.93, p < 0.05) and sex (p < 0.05). Based on the results, the most desirable game fish, walleye, appears less resilient to environmental variability than northern pike and yellow perch, making this species more susceptible to impacts of future climate change. 相似文献
75.
Knut Wiik Vollset Randi Ingebjørg Krontveit Peder A Jansen Bengt Finstad Bjørn Torgeir Barlaup Ove Tommy Skilbrei Martin Krkošek Pål Romunstad Arnfinn Aunsmo Arne J Jensen Ian Dohoo 《Fish and Fisheries》2016,17(3):714-730
Parasites can, in theory, have large impacts on the survival of fish populations. One method to evaluate such impacts on anadromous species is to apply manipulative field experiments in which parallel groups of antiparasitically treated and non‐treated fish are simultaneously released and then subsequently recaptured as returning adults. A systematic review and meta‐analysis on all such Norwegian studies on Salmo salar provided a data set for the time period 1996 to 2011 on 118 release groups comprising 657 624 fish released and 3989 recaptured. The overall risk ratio (RR) was estimated to be 1.18 (95% CI: 1.07–1.30). The effect varied strongly between groups, (Higgins I2 = 40.1%). Over 70% of this heterogeneity could be explained by the release location, time period and baseline survival. The most important predictor variable was baseline survival. In groups with low recapture in the control group (low baseline survival), the effect of treatment was high (RR = 1.7), while in groups with high recapture in the control group (high baseline survival), there was no effect of treatment (RR ~ 1.00). The most prevalent parasite in the region affected by the drugs administered was Lepeophtheirus salmonis. Hence, the meta‐analysis supports the hypothesis that L. salmonis contributes to the mortality of S. salar during outward migration. However, the effect of treatment was not consistent, but was evidently strongly modulated by other risk factors. The results suggest that the population‐level effects of parasites cannot be estimated independently of other factors affecting the marine survival of S. salar. 相似文献
76.
Raja Mansingh Rathore Bjørn Liaset Ernst Morten Hevrøy Adel El‐Mowafi Marit Espe 《Aquaculture Research》2010,41(11):e751-e759
This experiment aimed to test the interaction of lysine limitation with nutrient accretion and muscle carnitine depot in Atlantic salmon. Fish were fed adequate or low‐lysine diets for 3 months. Lysine intake was significantly less (48%) in fish fed the low‐lysine diet as compared with that fed the adequate one. There was no difference in dietary amino acids between treatments, with the exception of lysine. The lower lysine intake was reflected in plasma free lysine being 52% less while the free lysine concentration in the liver and muscle were unaffected. Although there was no significant difference between voluntary feed intakes among treatments, fish fed the low‐lysine diet had reduced growth, protein and energy deposition as compared with fish fed the adequate lysine diet. White trunk muscle contained more glycogen and less protein in fish fed the low‐lysine diet while no difference in lipid was observed. The livers from fish fed the low‐lysine diet contained less glycogen and slightly more fat and protein than the livers from fish fed the adequate lysine diet. Lysine limitation reduced carnitine in the liver without affecting muscle carnitine depot. Thus, low‐lysine diets did not likely affect the fatty acid oxidation capacity. This fact was supported by unaffected fatty acid profiles and lipid classes between treatments during the 3‐month study. In conclusion, lysine limitation does not deplete the muscle carnitine depot during the on‐growing seawater phase of Atlantic salmon, but affects the deposition pattern of nutrients. 相似文献
77.
In this study, exotoxins produced by 62 Aeromonas salmonicida strains and the bacterium Haemophilus piscium were analysed. Enzymatic assays, zymograms and serological detection were used to monitor secretion by bacterial strains of the previously described exotoxins P1, GCAT and AsaP1 and also the extracellular P2 metallo-gelatinase and a serine caseinase, which is different from the P1 protease and has not yet been characterized. Based on the results, the strains were divided into five groups. One comprised the type strains for A. salmonicida ssp. masoucida, H. piscium and 36% of the atypical isolates, and another, a type strain for A. salmonicida ssp. smithia together with 14% of the atypical isolates. A second type strain of A. salmonicida ssp. smithia was grouped with 8% of the atypical isolates. The largest group contained the type strains for A. salmonicida ssp. achromogenes and 38% of the atypical isolates. The type strains for A. salmonicida ssp. salmonicida were in the last group with all the four typical strains and 4% of the atypical isolates. The combination of zymogram and serological detection used is recommended as the most reliable method for characterizing A. salmonicida strains according to their exotoxin secretion. 相似文献
78.
The quality of compliance: investigating fishers’ responses towards regulation and authorities 下载免费PDF全文
A substantial amount of scientific effort goes into understanding and measuring compliance in fisheries. Understanding why, how and when fishers follow or violate rules is crucial for designing effective fishery policies that can halt overfishing. Non‐compliance was initially explained almost exclusively with reference to economic and self‐interested motivations. More recently, however, most explanations involve a combination of economic, social, political and environmental factors. Despite this recent development towards more holistic explanations, many scientists continue to frame the issue in binary terms: fishers either follow rules, or they don't. In this article we challenge this binary interpretation and focus attention on the diversity of fishers’ dispositions and perceptions that underpin compliant behaviour. To this aim we construct a typology of fishers’ responses towards regulation and authorities, thereby developing conceptual tools to understand different motivations and attitudes that underlie compliance outcomes. For this purpose, we identify the motivational postures of ‘creativity’ and ‘reluctance’, and then highlight their empirical relevance with an interview study of Swedish fishers. Reasons for studying the quality and diversity of fishers’ motivations and responses are not purely academic. Conceptualizing and observing the quality of compliance can help policymakers and managers gauge and anticipate the potentiality of non‐compliant fishing practices that may threaten the resilience of marine ecosystems. 相似文献
79.
Tracking escaped Atlantic cod (Gadus morhua L.) aggregated around Norwegian farms: Considerations for management strategies 下载免费PDF全文
R. D. Hedger R. M. Serra‐Llinares P. Arechavala‐Lopez R. Nilsen P. A. Bjørn I. Uglem 《Fisheries Management and Ecology》2017,24(4):265-273
To examine site fidelity and potential for ecosystem impacts, 20 escapee cod (Gadus morhua L.) from fjord‐based cod farms were tagged with acoustic transmitters, released in the vicinity of the same farm, and their subsequent behaviour and survival monitored using a fixed array of acoustic receivers. Most individuals demonstrated high fidelity to their farm where tagged, and this increased during the polar night. Only five individuals (25%) were occasionally detected at another farm (4 km distance), and only three cod (15%) migrated out of the fjord during the 6‐month study period. Vertical movements were consistently greater in the hours around noon than at night, a behaviour inconsistent with that observed for wild fish. A high mortality rate (40%) was observed during the first 4 months post‐release, and actual mortality may have been even higher, limiting the potential for negative ecosystem effects from cod escapees. Findings from this study are used to discuss the development of recapture strategies for minimising potential impacts from cod escapees to ensure sustainable farming activity. 相似文献
80.
Björn Baßmann Harvey Harbach Stephan Weißbach Harry W. Palm 《Journal of the World Aquaculture Society》2020,51(1):183-199
We investigated the effects of plant density on the welfare of African catfish, Clarias gariepinus, in coupled aquaponics over 85 days. The moderate density (mpd) of basil, Ocimum basilicum, was compared with the high density (hpd) and control (n = 0). The behavior was analyzed by visual and video observations, and after the application of induced stressors, skin injuries, blood glucose, lactate, and plasma cortisol responses were considered. The hpd fish showed the least activity (control: visual 77.8%, video 81.6%; mpd: 74.6%, 82.6%; hpd: 63.2% [p < 0.05], 78.8%). High agonistic behavior (control: 5, 131; mpd: 4, 57; hpd: 1, 45) and the highest number of injuries (control: 3.9; mpd: 2.9; hpd: 3.4) were observed in the control. Glucose and lactate levels did not differ significantly (control: 5.5, 2.6 mmol/L; mpd: 5.6, 2.7 mmol/L; hpd: 5.3, 2.6 mmol/L); however, cortisol levels did (control: 18.8 ng/mL, mpd: 19.9 ng/mL, hpd: 25.8 ng/mL). pH adjustment led to additional stress, resulting in temporal cortisol alterations. While in the control and mpd, low cortisol levels were followed by acute responses and downregulation, the hpd fish showed prior elevation and lagged an acute response. However, comparing injuries and behavioral patterns with control, aquaponics with high basil density influenced African catfish positively. 相似文献