基于生化数据处理模型的测控软件开发策略

为改变国产生化分析仪器测控软件功能单一的落后状况,针对非生化检测专业的软件开发人员提出了一种基于生化数据处理模型的测控软件开发策略。详细地分析了软件总体结构、测试机理和数据库操作方面的策略内容,对于生化数据的处理和分析提出了三种数据处理模型,并设计了其中一种程序流程。     

我国水稻空间诱变育种研究动态与展望

简要地综述了对水稻空间诱变育种的研究概况、取得的成果及对SP后代遗传变异规律的有关研究,分析了水稻空间诱变育种的方法;指出空间诱变育种是水稻育种的一条重要途径。     

NATURAL LANDSCAPES AND STRATEGIES OF REGIONAL SUSTAINABLE DEVELOPMENT IN EBINUR LAKE REGION IN XINJIANG

m0uurn0NTheecologicalintegrityOfallworidisdependedonourcomPrehensi0nandrepetOthestrUctUre,fonchonandchangsOflandscaPe.However,dwtngndalPrachcePr0Cess,theunmpdeveloPmenandmaremadediswtanceusuallycausethetwadationofenvironmenquality,andalsocausethech~soforiginalcharacteriSticsoflandscare.TheObjechveofthisstudyistorebulltaho-.mogeruzelandscapeStrUctUreandanimProvedecologicalenvironnent.Ebinurlakeregion(includeEbinurlake--thesecondlargestlakeinXinjiang)locatesinBortalaMongOliaAutonomousS…     

Dosage assessment of valnemulin in pigs based on population pharmacokinetic and Monte Carlo simulation

To estimate the valnemulin pharmacokinetic profile in a swine population and to assess a dosage regimen for increasing the likelihood of optimization. This study was, respectively, performed in 22 sows culled by p.o. administration and in 80 growing‐finishing pigs by i.v. administration at a single dose of 10 mg/kg to develop a population pharmacokinetic model and Monte Carlo simulation. The relationships among the plasma concentration, dose, and time of valnemulin in pigs were illustrated as C_i,v = X₀(8.4191 × 10^‐4 × e^?0.2371t + 1.2788 × 10^?5 × e^?0.0069t) after i.v. and C_p.o = X₀(?8.4964 × 10^?4 × e^?0.5840t + 8.4195 × e^?0.2371t + 7.6869 × 10^?6 × e^?0.0069t) after p.o. Monte Carlo simulation showed that T_>MIC was more than 24 h when a single daily dosage at 13.5 mg/kg BW in pigs was administrated by p.o., and MIC was 0.031 mg/L. It was concluded that the current dosage regimen at 10–12 mg/kg BW led to valnemulin underexposure if the MIC was more than 0.031 mg/L and could increase the risk of treatment failure and/or drug resistance.     

发酵血粉代替豆粕对育肥猪的影响

试验采用单因子试验设计,选用40kg左右的肥育猪40头,随机分成4组,每组5个重复,每个重复2头,分别饲喂基础日粮(对照组)和用发酵血粉分别替代基础日粮中的豆粕50%(试验Ⅰ组)、75%(试验Ⅱ组)和100%(试验Ⅲ组)等4种试粮,进行60d试验。结果表明:与对照组相比,试验Ⅱ组和试验Ⅲ组的末重与日增重均有显著提高(P<0.05),其中日增重分别提高11.33%和14.34%;与对照组相比,试验Ⅱ组料重比显著降低(P<0.05),其它各组差异不显著(P>0.05)。代谢试验表明:与对照组相比,蛋白质的消化率,氮沉积量(g/d)、氮沉积率(g/kgBW0.75)都显著提高(P<0.05)。随着替代的比例增加,经济效益改善呈上升趋势,试验Ⅱ组和试验Ⅲ组分别比对照组多赢利23.11%和28.29%。     

沸石粉、寡糖及益生素在黄鸡饲料中的应用效果研究

选取体质健康的新兴黄鸡5000羽,随机分成5个组,第1组为对照组,第2、3、4、5组每吨饲料中分别添加50kg沸石粉、100g低聚木糖、50kg沸石粉＋100g低聚木糖和50kg沸石粉＋100g低聚木糖＋100g地衣芽孢杆菌.本试验主要研究沸石粉、低聚木糖和地衣芽孢杆菌在黄羽肉鸡生产中的应用效果.结果表明：添加低聚木糖和地衣芽孢杆菌组肉鸡的生产性能皆优于对照组,其中第4和第5组分别比对照组的日增重高出3.2%和3.4%,第4、5组的料重比都分别比对照组降低3.2%和5.4%.血清抗氧化指标显示,试验组的抗氧化效果都比对照组好,且单独添加低聚木糖组T-SOD酶活显著高于对照组（P≤0.05）,第2、3、4、5组的MDA含量分别比对照组低12.3%、4.5%、9.4%和11.2%.试验组肉鸡肌肉中的胆固醇浓度都比对照组低,其中第2和第4组分别比对照组低15.4%和17.0%,且差异显著（P≤0.05）.     

Efficacy of different dosages of ivermectin injectable against the Hypoderma spp. in yaks

A chemotherapy trial was conducted to determine the lowest dosage of injectable preparation of ivermectin against Hypoderma spp. infestation in yaks in Tibetan areas in Tianzhu county, Gansu province, in northwest of China. One hundred and sixty yaks were randomly divided into four groups of 40 yaks for the trial. The first three groups were treated by subcutaneous injection in the neck with 0.1% ivermectin (respectively, 1, 5, 10 microg/kg body weight). The fourth group was not treated and considered as control group. All the experiments were performed in November 2000 and the animals were examined for the presence of warbles in the next March and May. The results indicated that there was no warbles found on the back of treated animal while third stage larvae were palpated on back of some of the yaks in control group. It is concluded that dosage of 1 microg/kg ivermectin injectable was sufficient to kill or stop development of larvae of Hypoderma spp. in naturally infected yaks if administrated in November.     

微滴式数字PCR定量检测鸡毒支原体方法的建立

为建立微滴式数字PCR（droplet digital PCR,ddPCR）定量检测鸡毒支原体（Mycoplasma gallisepticum,MG）方法,以MG的mgpc基因序列为目的序列,在其保守区域内设计合成了1对特异性引物和1条探针,通过各反应条件优化,建立了检测MG的ddPCR方法,并测定了建立方法的特异性、敏感性以及重复性。结果显示：建立方法的最佳引物浓度为20 μmol/μL,探针浓度为10 μmol/μL,退火温度为55 ℃;该方法只检出MG,没有检出鸡滑液囊支原体、禽衣阿华支原体、禽流感病毒、鸡新城疫病毒、鸡传染性支气管炎病毒、鸡传染性喉气管炎病毒、禽脑脊髓炎病毒、禽偏肺炎病毒、禽呼肠孤病毒、鸡沙门氏菌和鸡大肠杆菌,且相互间没有交叉反应;本方法最低检测MG重组质粒标准品的检测限为3.9拷贝/μL。重组质粒标准品浓度在3.9~41 025拷贝/μL范围内,绘制的ddPCR绝对定量曲线与测得值呈良好的线性关系（R2 = 0.999）;3次重复检测试验结果的变异系数均小于5%。对采集的60份病鸡喉拭子、气囊拭子及肺样品进行MG检测,结果ddPCR方法的阳性检出率（15.0%）高于荧光定量PCR方法（13.3%）。结果表明,本研究建立的ddPCR方法定量检测MG特异性强,灵敏度高,重复性好,为绝对定量检测MG提供了技术手段。     

中蜂品系间杂种优势利用研究初报

经过冬蜜花期对以湖北鄂西中蜂为母本、广东粤东地中蜂为父本的杂交一代与本地中蜂进行比较，结果表明：杂交一代的繁殖力和产蜜量分别比本地中蜂高4．4％和11％。夏季乌桕花期对回交代进行对比结果表明：其繁殖力和产蜜量分别比本地中蜂高6％和21％。     

日粮中多不饱和脂肪酸对动物免疫功能影响的研究

随着人民生活水平的提高，人们对安全、绿色食品的需求量越来越多。而磺胺类和抗生素类药物在动物生产中的应用非常广泛，随之带来的就是因药物残留引起一系列的食品安全问题。在日粮中添加多不饱和脂肪酸（PUFA）可以提高动物的免疫功能，从而减少动物患病的可能及动物生产过程中用药的机会。因此，多不饱和脂肪酸的应用可以为安全食品的提供做出贡献。本文对PuFA对动物免疫功能的影响作一综述。