Genetic diversity analysis of Phalaenopsis ‘Frigdaas Oxford’ using SRAP markers with reference to those genes responsible for variations in the pigmentation of petals and sepals

Summary

A selfed progeny from Phalaenopsis ‘Frigdaas Oxford’, with yellow flowers and red-purple blotches, was established. Individual plants varied greatly in terms of the number, size, and distribution pattern of the red-purple blotches. Sequence-related amplified polymorphism (SRAP) markers were used to analyse 159 individual plants in the progeny, and 14 polymorphic primer combinations were selected from the 594 SRAP primer combinations tested. In total, 80 polymorphic bands were produced using these primer combinations. On average, each primer pair combination amplified 80.1% polymorphic bands. An UPGMA dendrogram and a principal coordinate analysis (PCA) showed that the 159 individual plants selected could be divided into 12 clusters, including a cluster that consisted of plants in which the petals and sepals were fully red-purple in colour. Plants in which the petals and sepals had large red-purple blotches were genetically closer to the latter cluster than were those plants in which the blotches were small, indicating that the SRAP markers could be used efficiently to identify genetic variation in a Phalaenopsis population with respect to flower colour. Furthermore, 45 unique genes identified by SRAP from the selfed progeny population were sequenced. These data suggest that SRAP markers for the pattern of pigmentation in the petals and sepals of Phalaenopsis may be used in breeding Phalaenopsis for specific patterns of flower pigmentation.     

紫枝玫瑰（Rosa glaucca pourr）的引种栽培

为丰富我国北方寒冷地区彩色植物品种,黑龙江省森林植物园于1989年从丹麦引进紫枝玫瑰种子并进行播种育苗。十年的栽培试验研究发现,紫枝玫瑰在引种地生长良好,性状稳定,抗寒性强,无病虫害,是一种优良的四季观赏灌木。可通过扦插或播种方法进行繁殖。     

创建优质园林工程 标准规范应先行

从创建优质园林工程的角度,分析了园林工程诸环节存在的问题及其对工程质量的影响,探讨了各环节工作的规范化对工程质量的重要性。建议要提高园林工程质量,当务之急是要有严格的资质管理制度并制订一套与工程质量相关的切实可行的标准规范。     

油罐大修应注意的问题

目前我国每年有几十座油罐进行大修，平均每座油罐维修费需几十万元，大型油罐高，达几百万元，而不同地区，不同单位的大修周期长短不一，每年的大修费用总计高达几千万元，因此，科学地稳定油罐大修周期具有十分重要的意义。     

经鼻小柱,鼻中隔,蝶窦入路行脑垂体瘤切除术

经鼻小柱、鼻中隔、蝶窦入路行鞍内肿瘤切除术６例的结果表明：经鼻小柱、鼻中隔、蝶窦入路不但可避免唇下入路的某些并发症，且手术疗效一致。指出磁共振成像在垂体微腺瘤的定位诊断与指导肿瘤切除中具有重要价值，倡导神经外科与鼻喉科密切合作。     

巴比妥类镇静剂的检测研究进展

本文对巴比妥类安眠镇静剂检测方法的研究进展进行了综述。重点讨论了样品前处理方法及各种衍生化方法,GC、GC-MS、HPLC、HPLC-MS等检测方法。     

康德的批判哲学和哲学的批判精神

作为人类哲学思想的“蓄水池”,康德不仅以其批判哲学实现对传统哲学的根本变革,而且以其新哲学理念深刻影响现当代西方哲学的总体发展。康德的批判哲学开德国古典哲学之先河,是马克思主义哲学的历史渊源,其贡献在于倡导并实践批判的精神。然而康德的批判是不彻底的。当前,要坚持和发展马克思主义哲学,必须高扬“对现存的一切进行无情的批判”的精神,“在批判旧世界中发现新世界”。由康德所奠定的那种高瞻远瞩的理性批判精神,是永远值得人们珍惜和深思的。     

如何提高食品试验设计课程教学效果的探讨

食品试验设计与统计分析是一门理论性和实践性较强的课程,针对该课程既难教也难学的特点,对提高该课程的教学效果进行了探讨。