野外连续两年增强UV-B辐射对8个割手密无性系叶片MDA含量的影响*

 研究了大田栽培和自然光条件下，连续两年模拟UV-B辐射（280～315 nm，5.00 kJ/m²）增强对8个割手密无性系叶片丙二醛（MDA）含量的影响。UV-B辐射对割手密叶片MDA含量有显著的影响，8个割手密无性系叶片MDA含量对增强UV-B辐射存在种内差异。5个无性系叶片MDA含量有明显增加的趋势，分别为I91-97，I91-38，Ⅱ91-116，93-25，90-22；1个无性系MDA含量变化不明显（Ⅱ91-93），2个无性系MDA含量有降低的趋势，分别为Ⅱ91-98，92-11。UV-B辐射对割手密叶片MDA含量的影响具有年际间差异。UV-B辐射对割手密叶片MDA含量的影响存在生育期之间的差异，为伸长期>成熟期>分蘖期。     

连续3年UV-B辐射对33个割手密无性系成熟期锤度的影响

在大田栽培和自然光条件下，研究了连续3年模拟紫外辐射（UV-B，280～315nm）增加对33个割手密无性系成熟期锤度的影响。结果表明：（1）UV-B辐射对割手密无性系锤度具有明显的影响;（2）33个割手密无性系锤度对UV-B辐射响应具有明显的差异，在2003年、2004年和2005年，锤度响应范围分别为-27.86%～69.90%、-28.88%～34.58%和-18.90%～26.21%;（3）割手密无性系锤度对UV-B辐射响应具有明显的年际间差异，从选择的10个无性系来看，从2003年、2004年到2005年，6个无性系锤度变化率增加，4个无性系锤度变化率下降。     

连续3年UV—B辐射对10个割手密无性系株高、茎径的影响

研究了大田栽培和自然光条件下，连续3年模拟紫外辐射（UV—B，280～315nm）增加对10个割手密无性系伸长期株高和茎径的影响。结果表明：（1）割手密耐性无性系株高和茎径对UV—B辐射响应具有明显的差异；敏感无性系的株高和茎径显著或极显著的下降。（2）无性系株高和茎径对UV—B辐射的响应具有明显的年际、生育期之间的差异，在伸长初期和中期最敏感。（3）割手密无性系90—15的耐性最强，无性系Ⅱ91—93最敏感。     

Relationship between Protein Accumulation Regulation and Yield Formation in Soybean

Three different genotypes soybeans were adopted in this experiment under three fertilizer levels. The object of this study was to investigate protein accumulation regulation of soybean cultivars under the condition of different nutrient levels, and their effects on soybean yield and quality, and to provide theoretical evidence for breed, cultivation and agricultural production, also man-powered controllable locations. The concentration of N in the leaves declined after seedling stage, then increased again at stage of early flowering, and started to decrease up to leaf senescence, declined rapidly from seed-filling season to stage of yellow ripeness. The concentration of N in the stems and pod walls declined with growth stage. High seed protein genotypes exhibited higher N assimilating and partitioning during whole growth stages. Pod walls were media of N partitioning. Protein was accumulated mainly during the later period of reproductive growth stage up to harvest, so plant growth after stage of yellow ripeness could not be neglected.     

The Effects of Different Fertilizing Methods on Nitrification and Denitrification in Black Soil in Songnen Plain

The paper compared the effects of application of farm manure with chemical fertilizers on nitrification and denitrification in black soil, the result showed that the numbers of nitrobacterias and denitrobacterias in farm manure treatment were both higher than that of other treatments. The intensity of denitrification in chemical treatment was higher than that of manure treatment. The content of organic matter in soil was correlated with the intensity of nitrification and denitrification, and the coefficients were resnectively 0.9981 and 0.8693.     

ENVI FLAASH和ERDAS ATCOR2的大气校正对比研究

研究分别用ENVI FLAASH模块和ERDAS ATCOR2模块对LANDSAT5-TM图像进行大气校正,进而对校正效果进行评价。结果表明,ENVI FLAASH和ERDAS ATCOR2均能很好地消除大部分大气的影响,满足试验要求。但从精度方面考虑,ENVI FLAASH要略胜于ERDAS ATCOR2。     

合成条件对氧气氧化合成水钠锰矿的影响

A pot experiment was conducted to determine the dynamics of soil microbial biomass in a rainfed soil under wheat cultivation at the University of Arid Agriculture, Rawalpindi, Pakistan. The treatments applied were: 1) a control (CK), 2) NPK (0.44-0.26-0.18 g pot^-1), 3) farmyard manure (FYM, 110 g pot^-1), 4)poultry manure (PM, 110 g pot^-1), 5) FYM (110 g pot^-1) NPK (0.44-0.26-0.18 g pot^-1), 6) poultry manure (PM, 110 g pot^-1) NPK (0.44-0.26-0.18 g pot^-1), 7) FYM (110 g pot^-1) NPK(S) (0.44-0.26-0.18 g pot^-1 one half of the NPK at sowing and the other half one month after sowing), and 8) PM (110 g pot^-1) NPK(S) (0.44-0.26-0.18 g pot^-1, one half of the NPK applied at sowing and the other half one month after sowing). The experiment was laid out using a completely randomized design with three replications. Microbial biomass C, N and P contents increased continuously from the beginning of the experiment up to the three-leaf stage. A slight decline was observed at the tillering stage in all treatments except with the organic manures NPK(S) treatments. After tillering there was an increase in all treatments to the recorded maximum point at the full heading stage in all treatments except with the organic manures NPK(S) treatments. In the FYM NPK(S) and PM NPK(S) treatments; however, there was a continuous increase in microbial biomass up to the heading stage. At the harvesting stage a sharp decline was noted in all treatments. The C:N ratio of microbial biomass in tested soil ranged from 7.8 to 11.3, while C:P ratio of microbial biomass in the tested soil ranged from 22.6 to 35.1 throughout all growth stages of the wheat crop.     

一株抗灰霉病解淀粉芽孢杆菌的筛选鉴定及抑菌蛋白的分离

【目的】为研究一株筛选自大黑山林间土壤下的生防细菌,以形态学、16S rDNA进行鉴定。同时对其抑菌蛋白进行分离。【方法】采用对峙平板法进行初筛、复筛,用抑菌圈法测定抑菌效果,并镜检菌丝效果。通过形态学观察,生理生化及16S rDNA基因序列分析研究其分类。以SephacryI S-200分离抑菌蛋白。【结果】生防细菌K1对灰霉病具有稳定的抑菌效果,抑菌直径达到45 mm;16S rDNA鉴定为解淀粉芽孢杆菌。无菌发酵液通过SephacryI S-200分离得到一种抗菌蛋白。SDS-PAGE显示其分子量为55k Da。【结论】筛选出一株生防解淀粉芽孢杆菌K1,分离得到一种分子量为55k Da的抗菌蛋白。     

磷素水平对不同大豆品种氮素营养的影响

选用近年来黑龙江省推广面积比较大并具有代表性的3个大豆品种作为试验材料,采用盆栽,在N、K肥 施用量一定的基础上,设4个P处理,利用凯氏定氮法测定了不同大豆品种各器官氮素含量。结果表明,施磷对大 豆植株及各器官氮素含量有较大影响, 3个品种不同处理全株氮素含量从分枝期逐渐增加,成熟期达到高峰;同一 品种不同处理表现为适宜的磷水平有利于氮素的积累, 3个品种花期后都是P5 (每千克土施P2O5 0. 033g)处理全 株氮素含量最高,说明适宜的施磷有利于促进氮素积累。同一处理不同品种间高蛋白品种的氮素含量多于中间型 品种和高油品种,说明高蛋白品种需氮量多于中间型品种和高油品种。植株氮素含量高表现为单株产量和蛋白质 含量高,而脂肪含量却不是最高,氮素对脂肪形成影响较小。     

井灌水稻池内水温分布规律与灌水方法的研究

井灌水稻池内温度分布特征是靠近进水口处形成一低温区 ;灌水过程中温度变化是一个降温过程 ,停止灌水后水温迅速上升 ;采用单排单灌、间歇灌水、并且更换进水口的灌水方法 ,有利于降低因灌水造成池口附近低温区的不利影响 ;护胎水的管理以日间补水为宜