Effect of uric acid on the maturation and the biological function of BMDCs

AIM: To investigate the effect of uric acid on the maturation and the biological function of murine bone-marrow derived dendritic cells (BMDCs) in vitro.METHODS: BMDCs were cultured with GM-CSF, IL-4, LPS and uric acid. Cell phenotypes were analyzed by flow cytometry. MTT was used to detect the effect of uric acid on the function of BMDCs in stimulating the proliferation of T cells. IL-12 released by BMDCs was also detected. RESULTS: BMDCs were cultured and identified. Uric acid at concentrations of 200 mg/L and 400 mg/L increased the expression of the molecules (CD11c, CD83, CD86, IA/IE) on BMDCs surface and the IL-12 level in the culture supernatants (P<0.05), promoted the proliferation of T cells at the T: DC rate 5∶1, 10∶1, 20∶1 (P<0.05). However, uric acid at concentration of 70 mg/L had no effect on above molecule expression (P>0.05), no effect on T cell proliferation with BMDCs (P>0.05) was observed. CONCLUSION: Uric acid promotes the differentiation, maturation, the expression of co-stimulatory molecules, the IL-12 production in BMDCs and enhances the ability of BMDCs to stimulate the proliferation of T cell in a specical dose range.     

Changes of PKAC-β, c-Fos and BDNF in cerebral cortex after intracerebral hemorrhage in rats treated with WIN55-212-2

AIM: To observe the effect of cannabinoid receptor (CB1R) agonist WIN55-212-2 on the expression of brain-derived neurotrophic factor (BDNF), c-Fos and protein kinase A beta-catalytic subunit (PKAC-β) in cerebrum cortex after intracerebral hemorrhage (ICH) in rats. METHODS: The intracerebral hemorrhage model of rat was made by the injection of collagenase Ⅶ, and WIN55-212-2 was intraperitoneally (ip) injected 30 min later. The rats were killed for sampling the brain tissues as specimens 24 h after ICH. The methods of immunohistochemical analysis and Western blotting were adopted to detect the expression of PKAC-β and BDNF. The mRNA expression of PKAC-β, c-Fos and BDNF was determined by RT-PCR. RESULTS: WIN55-212-2 obviously improved some nervous deficit symptoms and increased the expression of BDNF at mRNA and protein levels with upregulating the mRNA expression of c-Fos and downregulating the expression of PKA at mRNA and protein levels in the ipsilateral cerebral cortex. The proteins of PKAC-β, c-Fos and BDNF were expressed on the membrane or nucleus of the neuron or in the cytoplasm of glial cells, respectively. CONCLUSION: The expression of BDNF is induced not only by upregulation of c-Fos, but also by downregulation of PKA in WIN55-212-2 treated rats.     

双孢菇漆酶酶学性质及染料脱色初探

对双孢菇漆酶的酶学性质及对染料的脱色性能进行了研究。结果表明:双孢菇漆酶的最佳反应温度和保存温度都是25℃,最佳反应pH和保存pH都是5.0,对大多金属离子不敏感,但Al3+对其有明显抑制作用。对8种工业染料中的4种脱色效果比较明显,其中弱酸艳蓝-RAWL的脱色率达到79%。     

甜瓜重组自交系群体第1雌花开花期遗传分析

以美国早花厚皮甜瓜品系WI998为母本（P1）,中国晚花薄皮甜瓜品系3-2-2为父本（P2）杂交所得的包括185个家系的重组自交系（RIL）群体为试验材料,通过RIL群体主基因+多基因混合遗传模型分析法,研究不同季节第1雌花开花期（DFF）的遗传特点。结果表明：春秋两季第1雌花开花期遗传均受2对重叠性主基因+加性多基因模型（E_1_8）控制,春季主基因遗传率为77.05%,多基因遗传率为22.44%;秋季主基因遗传率为72.38%,多基因遗传率为27.52%。表明甜瓜DFF主基因遗传力大于多基因遗传力,DFF主要受主基因控制,并在不同季节遗传稳定。     

Relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats

AIM:To elucidate the relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats (SHR).METHODS:Intracellular free calcium concentrations were measured by Fura 2 methodology and left ventricular function quantitated by cardiac catheterization in 20 SHR aged 10, 22, and 34 weeks and 20 age-matched Wistar-kyoto (WKY) rats.RESULTS:(1) The systolic blood pressure(SBP), intracellular calcium concentrations and left ventricular mass / body weight index (LVM/BW) were significantly higher in all three age groups of SHR than the corresponding groups of WKY; (2) Compared with age-matched WKY groups, the peak left ventricular pressure descending rate(-dp／dt_max) decreased while left ventricular relaxation time constant (τ)increased significantly in SHR aged 22 and 34 weeks. The peak left ventricular pressure ascending rate(dp／dt_max) and the left ventricular contractility index were significantly increased only in the 34 weeks SHR; (3) Intracellular calcium concentrations showed a positive correlation with LVM/BW,SBP,-dp／dt_max and τ(r=0.47-0.83,P<0.01)and a negative correlation with dp／dt_max and the left ventricular contractility index (r=-0.46,P<0.05 and r=-0.81, P<0.01).CONCLUSION:Intracellular calcium overload is one of the potential mechanisms in the induction of left ventricular hypertrophy as well as of systolic and diastolic dysfunction.     

LAMP检测无乳链球菌方法的建立及应用

以无乳链球菌纤连蛋白fbs基因为主要研究对象,采取环介导等温扩增技术(Loop-Mediated Isothermal Amplification,LAMP),针对fbs基因的6个区域设计4条特异性引物,利用一种链置换DNA聚合酶(Bst DNA polymerase)在63℃保温1 h,通过荧光显色即可完成对无乳链球菌的检测工作.结果显示,LAMP方法能够特异性检测fbs基因,其检测灵敏度是常规PCR方法的100倍,并与实时荧光定量PCR方法相当.所建立的针对无乳链球菌fbs基因的LAMP检测方法具有高度的特异性及稳定性,结果可靠,非常适合无乳链球菌的快速检测.     

黄秋葵品种比较试验

为筛选出适合萍乡地区种植的黄秋葵优良品种,为黄秋葵的进一步应用奠定资源基础,对10个黄秋葵品种进行了品种综合性状鉴定。结果表明:赣葵1号、赣葵3号、赣葵4号、枫桥秋葵生长势强,果实品质好,产量较高,2年平均667 m~2产量均在1 800 kg以上,可以大面积推广。赣葵4号、萍乡红葵的果实颜色鲜艳,可兼作观赏品种。     

温阳复方对肥胖大鼠模型糖代谢的影响

为考察温阳为主中药复方对肥胖大鼠糖代谢的影响,采用高脂饲料喂养12周建立肥胖大鼠模型,将造模成功的 SD 肥胖大鼠随机分成3组,分别为模型组、二甲双胍组、温阳复方组,并设正常对照组。连续灌胃8周,在给药第4、8周测其空腹血糖,给药 8周后测口服糖耐量与肌糖原、肝糖原、糖化血红蛋白含量。结果显示,给药 8周后,温阳复方组空腹血糖和糖化血红蛋白含量降低,肌糖原、肝糖原含量升高。结果表明,温阳复方能够明显纠正肥胖大鼠糖代谢紊乱,有效预防和延缓早期糖尿病的发生。     

AHP在联网审计系统中的应用

联网审计是依托网络平台才得以进行的审计形式,是互联网高速发展这个大环境下的必然产物.一个联网审计系统逻辑上包括3个部分:数据采集、数据传输和数据存储.被采集的数据需要经过网络传输,可采用的传输技术种类繁多,如SDH、ATM、帧中继FR和无源光网络APON、GPRS等,通过全面深入地研究,本文采用基于运筹学理论的层次分析法AHP对各种传输技术进行决策性分析,严谨地完成其数学计算,确立最佳的传输方案.