迪卡配套系猪RYR1的PCR分析及其利用的研究

试验从鸡东县永安猪场随机抽取迪卡配套系猪B系和E系42头，通过PCR扩增技术和酶切鉴定．采用克隆技术进行测序，来检测是否含有氟烷基因序列。经过以上技术检测这42头猪均没有氟烷基因。     

棉花冠层对不同灌水量的反应及其产量形成研究

通过探讨新疆北部棉区推广棉种新陆早6号与新陆早8号,在不同灌水量条件下冠层结构特征的差异性,得出新陆早6号对限量滴灌反应不敏感,而新陆早8号对限量滴灌反应较敏感。因此,在限量滴灌条件下,选择新陆早6号种植较适宜。叶面积指数低,叶片平均倾斜角度大,群体散射辐射透过系数和群体直射辐射透过系数增加,光能的截获率降低,由此得出,不同品种对灌水量的反应表现及理想群体冠层结构的合理指标,为增加棉花产量和提高水分利用效率提供了理论依据。     

王先谦汉学研究与书院传播

王先谦是晚清一位重要的汉学大师。受道光以后晚来湖湘汉学之风和督学江南时江浙考据学派的影响 ,王先谦的治学呈现明显的汉学倾向 ;在湖湘理学传统和晚清经世思潮的影响下 ,王先谦的汉学又不同于传统的乾嘉汉学 ,已走出纯粹的考据而呈现出既趋重经史考据 ,又能调和汉宋、兼采古今、求实致用的多途取向。王先谦一生治学讲学 ,始终没有离开过书院。正是借由如南菁、思贤、城南、岳麓等书院 ,王先谦为传播汉学做出了重要贡献。     

日粮锌、硒水平对肉鸡肠道黏膜屏障结构的影响

为了探讨微量元素锌和硒相互作用对肉鸡肠道黏膜屏障结构的影响，将24只1日龄AA肉鸡随机分3组，分别饲喂添加有高锌高硒(锌1000mg／kg、硒5mg／kg)、低锌低硒(锌34mg／kg、硒0．08mg／kg)或常锌常硒(锌50mg／kg、硒0．15mg／kg)的日粮45d后，观察肠黏膜上皮细胞、上皮内淋巴细胞和盲肠扁桃体的形态结构变化。结果表明：高锌高硒或低锌低硒组肉鸡的肠黏膜结构有明显的损伤，表现为肠黏膜上皮细胞萎缩，绒毛长度下降，上皮内淋巴细胞数量减少；盲肠扁桃体的弥散淋巴组织和淋巴小结中，淋巴细胞数量减少，细胞出现肿胀，有的核消失，结缔组织增生，淋巴小结萎缩。尤其是高锌高硒组的损伤最为严重。而常锌常硒组肉鸡肠黏膜和盲肠扁桃体的形态结构正常。结论：日粮中按锌50mg／kg、硒0．15mg／kg的比例添加，对于维持肠道黏膜的正常屏障结构是合适的。过高或过低的锌和硒对小肠黏膜有毒性作用，破坏其屏障功能；而且高锌和高硒可相互促进以增强其毒性作用。     

猪CD3∈分子的cDNA克隆与序列分析

应用RT-PCR和3′RACE技术,本研究从猪外周血淋巴细胞总RNA中扩增克隆了猪CD3ε基因并进行了序列分析。序列分析结果表明,猪CD3εcDNA序列长1241nt,其中5′非翻译区80nt,3′非翻译区570nt,开放阅读框591nt,该开放阅读框编码196个氨基酸残基的CD3ε前体蛋白(无糖基化位点);在猪CD3ε蛋白的胞外区,Cys49、Cys91、Cys108和Cys111为保守性氨基酸残基,它们与CD3ε免疫球蛋白样结构的形成有关。在猪CD3ε蛋白胞浆区,存在免疫受体酪氨酸活化基序(ITAM)、内质网潴留基序和1个SH3结合基序,其中ITAM基序含有2个SH2结合基序和2个潜在酪氨酸磷酸化位点Tyr177和Tyr188,这些基序是猪CD3分子参与T细胞活化信号转导和TCR-CD3复合体装配的结构基础。推导氨基酸序列分析结果表明,猪与人、鼠、狗、牛和绵羊CD3ε蛋白的氨基酸同源性分别为61.2%、58.7%、58.7%、65.1%和65.6%。     

加强高校领导班子思想政治建设的若干思考

党的十六届四中全会作出了《关于加强党的执政能力建设的决定》,第十三次全国高校党建工作会议提出了全面加强高校领导班子的思想政治建设的要求。加强高校领导班子思想政治建设,必须紧紧抓住理想信念、政治方向、立场、宗旨观念、治校能力、自身修养等关键环节,不断提高领导干部的自身素质和领导水平,增强领导班子的凝聚力、创造力、战斗力。     

竹材刨花板用作混凝土模板的试验

在实验室条件下研究了混凝土浇筑过程和重复周转使用对竹材刨花板性能的影响。试验表明,采用竹材刨花板作为混凝土模板是可行的,重复周转使用4次是安全的;竹材刨花板作为底模板,在浇筑混凝土的过程中,受弯曲的挠度在前20 h左右呈现增长,20~60 h为下降阶段,60 h后恢复到略高于初始的挠度;重复周转使用6次后,静曲强度衰减了31.7%、弹性模量衰减42.4%、内结合强度衰减65.1%、厚度膨胀率增长到3.1%。     

水胺硫磷在早熟苹果辽伏中的残留习性

对早熟苹果辽伏喷布40％水胺硫磷1000倍液。喷后27d,果皮及果肉中残留量分别降至0.182mg·1~(-1)和0.028mg·1~(-1)。果皮中水胺硫磷残留含量一直呈下降趋势,但果肉中水胺硫磷含量在喷后前7d却在增加,第7天之后才呈下降。经对果皮及果肉中水胺硫磷含量的差比分析认为,果肉中水胺硫磷含量在喷后前7d增加,是果皮中农药向果肉中渗透大于果肉中农药消解所致。     

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AIM: To investigate the contribution of angiotensin-converting enzyme inhibitor (ACEI) to the regulation of calpain system in infarcted myocardium. METHODS: Rat myocardial infarction (MI) model was established by permanent ligation of the left coronary artery. The treatment with the ACEI inhibitor rampril (1 mg·kg-1·d-1) was started 7 days prior to surgery. On day 1, 3, 7 and 14 after MI, protein levels of calpainⅠ, Ⅱ and calpastatin were determined in left ventricular free wall (LVFW), interventricular septum (IS) and right ventricule. RESULTS: CalpainⅠprotein level was increased in IS 14 d post MI, whereas the protein level of calpainⅡ was maximally increased in LVFW 3 d post MI. Rampril decreased protein up-regulation of calpainⅠ and Ⅱ, and reduced infarct size and interstitial fibrosis. Calpastatin protein expression was not affected by ACEI. CONCLUSIONS: CalpainⅠ is involved in cardiac remodelling in the late and calpainⅡ contributes to cardiac tissue damage in the early phase of MI. The heart protective effect of ACEI may be related to the inhibition of calpain system in the pathogenesis of myocardial infarction.     

Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats

The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis.