不同隔离层材料对‘兰引Ⅲ号’结缕草无土草皮质量的影响

以‘兰引Ⅲ号’结缕草为试材,进行无土栽培试验,研究了4种不同的隔离层(遮阳网、无纺布、地膜、砖块)材料对‘兰引Ⅲ号’结缕草草皮质量的影响;通过定期测定草坪盖度、密度、颜色及成坪后的草皮强度,以便筛选出适合的无土草皮生产的隔离材料。结果表明:建植70d后遮阳网隔离层草皮的盖度为92.78%、100cm2密度为81.34枝、色泽为6.67,均高于其余隔离层材料,生长过程整体表现为遮阳网砖块无纺布地膜;砖块的草皮强度为5.98kg,显著高于其余3种隔离层材料,但砖块隔离层与遮阳网隔离层相比建设成本太大。综合考虑,认为遮阳网隔离层比较适合‘兰引Ⅲ号’结缕草无土草皮的生产,而地膜的排水透气能力较差,不适宜作为‘兰引Ⅲ号’结缕草无土草皮生产的隔离层材料。     

日光温室果树台田式限根栽培温度变化规律的研究

通过对日光温室台田式限根槽栽培方式的地温与气温变化规律研究.结果表明:温室内气温和地温最低值出现时间一致;气温变化快,日变幅大;地温受气温影响,变化明显滞后;随着土层加深,地温日变幅变小;升温初,台田较平作地温上升快.覆盖白色和黑色地膜都能明显提高地温.土层越浅并且距离限根槽边缘越近,地温受气温影响越大;随土层加深各部位地温差减小.台田式限根栽培有利于迅速提高地温,协调地温和气温关系.     

茶树CsSPMS基因全长cDNA克隆和时空表达分析

为探究精胺合成酶（spermine synthase,SPMS）与茶树（Camellia sinensis）耐寒性的关系,以茶树品种‘迎霜’为试验材料,利用简并引物PCR扩增技术结合5′/3′RACE方法,获得与低温胁迫相关的CsSPMS片段cDNA全长序列（GenBank登录号为KJ580429）。该序列全长1 374 bp,包含1 113 bp的完整开放阅读框（ORF）,编码371个氨基酸,预测分子量41.28 kD;构建亚细胞定位载体pJIT166-GFP/SPMS,亚细胞定位结果显示CsSPMS定位在细胞核上。荧光定量PCR分析表明CsSPMS在根、茎、叶、芽、花和果中都有表达,在根、茎中表达量较高;低温胁迫处理下不同组织CsSPMS的表达量,在叶片中2 h达到峰值,而在根中12 h达到高峰;在低温条件下4个茶树品种的耐寒性与CsSPMS表达量密切相关。     

外源一氧化氮对盐胁迫下山葡萄叶片叶绿素荧光特性的影响

采用营养液水培试验,研究了外源一氧化氮(NO)供体硝普钠(SNP)处理对盐胁迫下山葡萄‘双丰’1a生扦插苗叶片叶绿素荧光特性的影响。结果表明:外源NO提高了盐胁迫下山葡萄叶片PSⅡ的最大光化学效率(Fv/Fm)、PSⅡ实际光化学效率(ΦPSⅡ)和光化学猝灭系数(qP),降低了PSⅡ的激发能压力(1-qP);另外,在一定程度上提高了盐胁迫下最大潜在电子传递速率(rETRm)、半饱和光强(Ik)和快速光曲线的初始斜率(α)。表明外源NO通过减少过剩激发能的压力,提高了光合电子传递效率,减轻不可逆光抑制,进而缓解盐胁迫对山葡萄光合机构的伤害,提高其耐盐性。     

Effect of transplantation of hMSCs modified by BDNF and GDNF gene on injured brain in rat MCAO model

AIM：To study the therapeutic effect of human mesenchymal stem cells (hMSCs) modified by brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) gene transfer with liposome on middle cerebral artery occlusion (MCAO) model rats. METHODS：The nonviral expression vector was constructed and transfected into the hMSCs by liposomal method. The rat brain injury model was established by the method of MCAO. The gene-modified hMSCs, control hMSCs or PBS was transplanted into the rats 24 h after MCAO by femoral venous injection. The neurological function score, the change of the body weight and the behavior test were used to evaluate the damage of the brain in the rats. The degree of the damage and the migration of the cells 15 d after transplantation were analyzed by observing the histological changes of the brain tissues. RESULTS：The expression levels of BDNF and GDNF in gene-modified hMSCs were much higher than those in control hMSCs. The transplantation of BDNF and GDNF gene-modified hMSCs promoted the functional recovery and reduced the infarct size in the rats after MCAO. A few exogenesis cells only survived in the infarct area of the brain in the MCAO rats, and the cells showed no differentiation. CONCLUSION：Transplantation of BDNF and GDNF gene-modified hMSCs by nonviral expression vector is effective in treating cerebral ischemia. The effect may result from the action of the cytokines secreted by these cells, reducing the injuries induced by the brain ischemia and accelerating the nerve repair following the injury.     

Effects of exosomes derived from hypoxia-preconditioned umbilical cord mesenchymal stem cells on function of endothelial cells

AIM To investigate the effect of exosomes derived from hypoxia-preconditioned human umbilical cord mesenchymal stem cells (hUCMSCs) on proliferation, migration and tube formation of human umbilical vein endothelial cells (HUVECs). METHODS hUCMSCs and HUVECs were isolated, cultured and identified. Exosomes derived from hUCMSCs were extracted by ultracentrifugation. The morphological change of exosomes was observed under transmission electron microscope. The particle size and concentration of exosomes were detected by nanoparticle tracking analysis, and the surface specific marker proteins of exosomes were determined by Western blot. hUCMSCs were divided into normoxia group and hypoxia group. The viability of hUCMSCs was measured by CCK-8 assay. HUVECs were divided into control group, normoxic exosome group and hypoxic exosome group. The proliferation of HUVECs was detected by EdU assay. The migration ability was detected by cell scratch assay and Transwell experiment. Tube formation ability was evaluated by tube formation experiment. RESULTS Compared with normoxia group, hypoxia pretreatment enhanced the viability and exosome release of hUCMSCs. Compared with normoxic exosome group, hypoxic exosomes enhanced the proliferation, migration and tube formation of HUVECs. CONCLUSION Exosomes derived from hUCMSCs under hypoxia enhances the proliferation, migration and tube formation of HUVECs.     

玉米间作菊花脑高效栽培方法对玉米杂草的影响

研究了玉米间作菊花脑的高效栽培方法对玉米杂草的影响,以探究此间作方法对杂草的防效及间作经济效益,为在生产实践中进一步推广应用提供理论依据.结果表明,玉米菊花脑间作处理(T)较玉米单作处理(CK),玉米产量增加,每667 m2产值增加2496元,增收效果明显;间作处理的玉米维生素C、粗淀粉、可溶性糖、可溶性蛋白含量分别提...     

282例成人泌尿生殖道支原体感染调查及药敏分析

目的调查分析我院解脲支原体(Uu)和人型支原体(Mh)感染泌尿生殖道情况及9种抗生素耐受性变化,为临床合理用药提供科学的参考依据。方法应用支原体培养鉴定药敏试剂盒对泌尿生殖道感染患者生殖泌尿道拭子进行体外支原体培养、鉴定及药物敏感试验。结果282例标本中99份标本培养出支原体,阳性率为35.11%。其中单纯Uu阳性90例,阳性率为31.91%;单纯Mh阳性1例,阳性率为0.36%;Uu合并Mh阳性8例,阳性率为2.84%。女性感染支原体的阳性率为37.69%,明显高于男性的4.55%(P<0.01)。单纯Uu感染株对交沙霉素、强力霉素和原始霉素敏感度相对高于其他抗生素。结论支原体是导致性传播泌尿生殖道感染常见的病原体之一;我院支原体感染以Uu为主,药敏以大环内酯类药物效果最好,以交沙霉素、强力霉素和原始霉素为首选。     

槟榔间作香露兜对土壤养分和养分吸收的影响

为了研究槟榔间作香露兜对土壤养分和养分吸收的影响,采用盆栽试验的方法,对槟榔单作、槟榔间作香露兜和香露兜单作3种种植模式的土壤理化性质和养分吸收进行比较研究。结果表明：槟榔间作香露兜后,相对于单作槟榔,土壤EC值和碱解氮含量显著降低47.15%和25.74%,土壤速效磷含量显著增加32.32%,其中碱解氮与过氧化氢酶和过氧化物酶极显著负相关,速效磷与总根长、根系总表面积、根体积、根数目、脲酶和酸性磷酸酶显著正相关,速效磷与过氧化氢酶和过氧化物酶极显著正相关;槟榔地上部N浓度以及香露兜地上部P和K浓度分别显著增加41.56%、26.56%和25.69%;而槟榔和香露兜根系以及地上部N、P和K养分含量均高于单作,其中根系槟榔P和K含量和香露兜K含量显著增加;同时,间作后槟榔和香露兜对N的吸收效率均高于单作,香露兜的P和K的吸收效率高于单作并略高于槟榔的吸收效率,其中P和K的吸收效率与总根长、总表面积、根体积、根数目均显著正相关,氮的吸收效率与根系形态无显著相关关系。综上所述,槟榔间作香露兜增加了土壤中速效磷含量,促进了槟榔和香露兜对氮、磷和钾养分的吸收,土壤速效磷含量的增加、养分吸收效率的提高均与根系形态显著正相关。     

我国区域现代农业发展途径与战略重点

发展现代农业是推进农业现代化的必然要求和新农村建设的首要任务,我国幅员辽阔,不同区域应有相应的区域发展重点与布局。本文从剖析国家四大战略对区域农业发展的定位入手,指出区域农业战略制定必须服务于国家的区域目标,在此基础上,深入探讨了未来区域现代农业发展的思路、战略目标、功能定位、战略重点与战略布局。