全文获取类型
收费全文 | 22874篇 |
免费 | 1446篇 |
国内免费 | 2294篇 |
专业分类
林业 | 1959篇 |
农学 | 1808篇 |
基础科学 | 1417篇 |
2775篇 | |
综合类 | 9887篇 |
农作物 | 1518篇 |
水产渔业 | 976篇 |
畜牧兽医 | 3599篇 |
园艺 | 1537篇 |
植物保护 | 1138篇 |
出版年
2024年 | 161篇 |
2023年 | 416篇 |
2022年 | 1113篇 |
2021年 | 1133篇 |
2020年 | 1164篇 |
2019年 | 1058篇 |
2018年 | 776篇 |
2017年 | 1143篇 |
2016年 | 886篇 |
2015年 | 1217篇 |
2014年 | 1302篇 |
2013年 | 1430篇 |
2012年 | 1932篇 |
2011年 | 1991篇 |
2010年 | 1898篇 |
2009年 | 1684篇 |
2008年 | 1519篇 |
2007年 | 1410篇 |
2006年 | 1077篇 |
2005年 | 899篇 |
2004年 | 494篇 |
2003年 | 279篇 |
2002年 | 310篇 |
2001年 | 300篇 |
2000年 | 255篇 |
1999年 | 164篇 |
1998年 | 71篇 |
1997年 | 72篇 |
1996年 | 89篇 |
1995年 | 57篇 |
1994年 | 42篇 |
1993年 | 48篇 |
1992年 | 50篇 |
1991年 | 32篇 |
1990年 | 29篇 |
1989年 | 26篇 |
1988年 | 28篇 |
1987年 | 19篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 6篇 |
1963年 | 2篇 |
1962年 | 2篇 |
1956年 | 11篇 |
1955年 | 4篇 |
1905年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
51.
试验旨在构建能表达牛病毒性腹泻病毒(Bovine viral diarrhea virus,BVDV)E2抗原蛋白的重组乳酸乳球菌(Lactococcus lactis),为进一步研制BVDV乳酸菌口服活载体疫苗奠定基础。将BVDV E2基因克隆后测序,根据乳酸乳球菌的密码子偏嗜性进行优化,再将优化的基因片段插入表达载体pNZ8148中,并电转化乳酸乳球菌NZ9000感受态细胞,构建重组乳酸菌pNZ8148-E2/NZ9000,经1 ng/mL乳链菌肽诱导表达后,对菌体物进行了SDS-PAGE和Western blotting分析。将重组乳酸菌pNZ8148-E2/NZ9000口服免疫6~12月龄健康犊牛,在免疫后不同时间点采集血液样品并分离血清,用间接ELISA方法检测抗体水平。结果显示,PCR扩增到了1 149 bp的目的片段,乳酸菌密码子偏嗜性优化后,GC含量从45.28%变为34.30%。重组质粒pNZ8148-E2经酶切鉴定插入片段与预期大小相符,在菌体裂解物中出现大小约42 ku的条带,与预期蛋白大小一致,且该蛋白可与BVDV E2抗体反应。在免疫犊牛的血清中检测到特异性抗BVDV E2蛋白的抗体。本研究结果表明,表达BVDV E2蛋白的重组乳酸菌口服免疫可诱导犊牛产生特异性的体液免疫反应,该重组菌具有较好的免疫原性。 相似文献
52.
53.
为探明拔节期氮肥运筹对不同滴灌量下冬小麦光合特性及产量的影响,在大田滴灌技术条件下,以‘新冬41号’为试验材料,研究4种拔节期氮肥运筹处理[N0(0 kg/hm2)、N1(90 kg/hm2)、N2(180 kg/hm2)、N3(270 kg/hm2)]对3种不同滴灌量[W1(1500 m3/hm2)、W2(3000 m3/hm2)、W3(3450 m3/hm2)]下冬小麦叶绿素含量(SPAD值)、叶面积指数(LAI)、叶片光合特性及产量的影响。结果表明,在3种滴灌量下各氮肥处理冬小麦拔节至乳熟期叶片SPAD值均呈“先增后降”的变化规律,最大值均在灌浆期;LAI呈现“先增后降”的变化规律,在孕穗期达最大。在同一施氮水平下,滴灌量增加,叶片的净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)均增加,而胞间CO2浓度(Ci)则降低。滴灌量较低时(W1处理),增加拔节期施氮量能有效提高滴灌冬小麦的有效穗数、穗粒数、千粒重、产量和收获系数;水分充足时(W2、W3处理),增加拔节期施氮量反而不利于产量的提高。综合考虑各项产量指标及氮肥利用效率得出,在滴灌量3000 m3/hm2条件下,拔节期施氮量180 kg/hm2时,滴灌冬小麦籽粒产量较高,氮肥利用率最大,可供生产参考。 相似文献
54.
55.
AIM: To investigate the effect of microwave radiation at different intensities on the rat myocardium and its possible mechanism.METHODS: The rats were radiated by the intensity of 500, 1 000, 1 500 and 2 000 W/m2 with 2 450 MHz microwave for 6 min. The heart tissue was collected 6 h after microwave radiation. ATP and mitochondria complex Ⅳ and Ⅴ were measured. The changes of the tissue structures were observed under transmission electron microscope. The apoptosis of the myocardial cells was detected by a cell analyzer. The protein level of cleaved caspase-3 was determined by Western blotting.RESULTS: The concentration of ATP and activity of mitochondria complex Ⅳand Ⅴ signi-ficantly decreased compared with control group in the cardiac tissues. The decreased number, morphological abnormalities such as dissolved cavitation, matrix and obvious tumefaction of mitochondria were observed under transmission electron microscope. The microwave radiation induced the apoptosis of myocardial cells in the rats. The cell apoptotic rate and the protein level of cleaved caspase-3 increased with increasing intensity of microwave radiation(P<0.05).CONCLUSION: Microwave radiation has obvious injury effect on the rat heart, which can cause cardiac energy metabolism dysregulation and cardiac myocyte apoptosis. 相似文献
56.
57.
5 male Small-tail Han sheep with the body weight of 45 kg at 1.5 years old,fitted with permanent rumen fistula,to study of the effect of the supplement of the untreated commercial lysine on the rumen microbiocoenosis,rumen metabolism and whole body metabolism by 5×5 Latin square design (5 groups:control,control with low urea (equal nitrogen),low lysine,control with high urea (equal nitrogen),high lysine).The amounts of lysine-HCl supplement were 0,4.0 and 8.0 g/kg diet (as DM basis) in each group respectively,with two urea supplement control groups of equal amount of nitrogen.The results showed that by supplement of 4.0 and 8.0 g/kg of lysine-HCl diet (as DM basis) respectively,the voluntary intake of dry matter of sheep were increased by 5.5%(P>0.05) and 11.8%(P<0.05) respectively.The total amount of rumen bacteria were increased by 18.9%(P<0.01) and 23.9%(P<0.01) respectively,as the amount of rumen Coccus was increased by 21.2%(P<0.01) and 30.1%(P<0.01) respectively.The amount of rumen big Bacillus was decreased by 16.7%(P<0.05) and 33.3%(P<0.01) respectively.The total VFA of was increased by 9.6%(P<0.05) and 12.2%(P<0.01) respectively,as the butyric acid was increased by 12.8%(P>0.05) and 20.2%(P<0.01) respectively.The apparent digestibility of organic matter was increased by 6.4%(P>0.05) and 10.0%(P<0.05) respectively,the apparent digestibility of crude protein was increased by 7.0%(P>0.05) and 18.3%(P<0.01) respectively;And nitrogen retention was increased by 46.4%(P>0.05) and 110.7%(P<0.01) respectively.However,there was no effect of lysine supplement on the amounts of the rumen protozoa and fungi,and the concentration of rumen ammonia nitrogen.It was concluded that the supplement of untreated commercial lysine to adult sheep would increase the voluntary intake,digestibility,nitrogen retention the rumen microbiocoenosis and the total amount of rumen bacteria,especially the rumen Coccus of adult sheep.The effect of lysine supplement could not be replaced by urea supplement completely. 相似文献
58.
59.
60.