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71.
The biological effects of ELFMES including proliferation and apoptosis of cells as well as the damage of DNA are initially evaluated with the treatment of plant cells and human pulmonary fibroblasts in low frequency magnetic fields with certain intensity and different time intervals in vitro. Cell culture, counting in vitro, fluorescent dyeing analysis and single cell gel electrophoresis are adopted. The results demonstrate that the electromagnetic field with the intensity of 3mT can affect the proliferation and apoptosis of cells significantly. 相似文献
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‘金美’是从云南野外美味猕猴桃资源中选育出的绿肉高糖新品种。果实为卵形,果面黄褐色硬毛,果形整齐;平均单果质量60 ~ 80 g。果肉绿色或黄绿色,质嫩多汁,风味浓甜。软熟果实含可溶性固形物24.1%,可溶性总糖16.2%,总酸1.25%,维生素C 1 180 mg • kg-1,钾 342 mg • kg-1,钙84.2 mg • kg-1。在武汉地区8月果实成熟,此时果实采后7 d开始软熟,1 ~ 2 ℃低温和95%相对湿度下可存放100 ~ 120 d。盛果期产量22 t • hm-2。 相似文献
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梧桐花黄酮的提取及其抑菌、抗病毒效果 总被引:1,自引:0,他引:1
以10倍原料质量的60%乙醇在75℃条件下对梧桐花进行浸提,并对总黄酮的含量进行了测定.为了研究梧桐花总黄酮对多种常见畜禽病原的体外抑菌、抗病毒作用,采用培养基打孔法,观察梧桐花总黄酮对致病菌的抑菌作用;采用试管法,检测梧桐花总黄酮的最小抑菌浓度;采用细胞培养技术,检测梧桐花总黄酮对新城疫病毒(NDV)和法氏囊病病毒(IBDV)在细胞内增殖的抗病毒作用.结果显示,梧桐花总黄酮对供试菌均具有抑菌作用,其中对四联球菌、八叠球菌、大肠杆菌、禽波氏杆菌的抑菌效果最好,抑菌直径D>16 mm;梧桐花总黄酮在3.125 g/L时能够100%抑制NDV在CEF内增殖;梧桐花总黄酮在1.563 g/L时能够100%抑制IBDV在CFE内增殖;梧桐花总黄酮在体外质量浓度为1.563 g/L时可直接灭活所有NDV;梧桐花总黄酮在体外质量浓度为0.781 g/L时可直接灭活所有IBDV.结果表明,梧桐花总黄酮具有体外抑菌和抗病毒作用,为将其研制成新型、绿色环保的中草药制剂提供了试验基础. 相似文献
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[目的]探讨不同施氮方式和灌水量对甘蔗生长、养分累积及产量的影响,为合理利用水肥资源实现甘蔗高产高效栽培提供参考依据.[方法]以新台糖22号(ROC22)为试验材料,设不施氮肥、不灌水(CK),氮肥作基肥一次性施用、灌水750 mm(T1),氮肥作基施一次性施用、灌水1000 mm(T2),氮肥作基施一次性施用、灌水1250 mm(T3),氮肥分3次施用(基肥30%、分蘖肥30%、攻茎肥40%)、灌水750 mm(T4),氮肥分3次施用、灌水1000 mm(T5)和氮肥分3次施用、灌水1250 mm(T6)7个处理,测定各处理甘蔗农艺性状、各部位养分含量、产量和蔗汁锤度等指标,研究不同施氮方式和灌水量对甘蔗生长与产量的影响.[结果]中、高灌水量(1000和1250 mm,下同)时,分3次施氮处理甘蔗的株高高于一次性施氮处理,在5~7月差异显著(P<0.05).在甘蔗成熟期,茎干重表现为T1>T2>T3、T6>T4>T5、T1>T4、T2>T5和T3T4、T2>T5和T3T2>T3、T6>T4>T5、T1>T4、T2>T5和T3T1>T2、T4>T6>T5、T1相似文献
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介绍了使用多普勒天气雷达对濮阳2005年7月22日暴雨天气过程进行连续监测的情况,利用基本反射率、组合反射率和回波顶、垂直积分液态水、基本速度等雷达产品进行分析。结果表明,此次降水回波持续时间较长,有"人"字形回波特征。径向速度产品图上,逆风区与辐合区出现、发展,预示强风暴的出现。速度场的强弱变化早于回波强度变化,可更早得到风暴的发展情况,追踪多普勒径向速度场上的逆风区、辐合区的运动路径,可以大致确定强降水的落区,从而提前预报出灾害性天气。垂直积分液态含水量相对稳定,大值区与强回波区有一定的对应关系。通过分析得出此次暴雨的一些雷达回波特征,可为今后暴雨识别、预报提供参考和依据。 相似文献
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AIM:To observe the expression of tumor necrosis factor α (TNF-α) and nuclear factor κB (NF-κB) in the renal tissue of the rats with contrast-induced nephropathy (CIN). METHODS:Male Sprague-Dawley rats (n=96) were randomly divided into control group (n=48) and CIN group (n=48). The model rats in CIN group were intravenously injected with iodinated contrast media (76% compound diatrizoate injection,10 mL/kg), while the rats in control group were injected with the same volume of saline. Six rats in each group were sacrificed at 6 h, 12 h, 24 h, 48 h, 72 h, 5 d, 10 d and 15 d after intravenous injection, respectively, and the blood and kidney samples of the rats were obtained. The renal tubular injury was assessed by histological examination (HE staining). The expression of kidney injury molecule-1 (KIM-1), TNF-α and NF-κB at mRNA and protein levels in the renal tissues were semiquantitatively measured by the methods of RT-PCR and immunohistochemistry, respectively. The correlations between the expression of TNF-α, NF-κB and tubular injury score, KIM-1 expression in renal tissue of CIN group were analyzed. RESULTS:The levels of serum creatinine (SCr) and blood urea nitrogen (BUN) in control group were not changed between different time points (P>0.05). The levels of SCr and BUN in CIN group displayed significant increases at different time points (except 15 d) compared with control group (P<0.05). The renal tubular injury score in CIN group was significantly higher at all time points than that in control group (P<0.05). The expression of KIM-1, TNF-α and NF-κB at mRNA and protein levels up-regulated significantly at 6 h and the peaking of KIM-1 expression was at 24 h, while the peaking of TNF-α and NF-κB expression was at 48 h in CIN group. The expression of KIM-1,TNF-α and NF-κB was significantly increased in CIN group compared with control group except at 15 d (P<0.05). The expression of TNF-α and NF-κB at mRNA and protein levels showed close correlations with renal tubular injury score (r=0.843, 0.758, 0.743 and 0.707, P<0.05). The expression of TNF-α and NF-κB at mRNA and protein levels was also positively correlated with KIM-1 expression (r=0.863, 0.807, 0.839 and 0.855, P<0.05). CONCLUSION:The expression of TNF-α and NF-κB at mRNA and protein levels in the renal tissues of CIN group is up-regulated and is closely related with renal tubular injury, indicating that the inflammatory response is involved in the pathogenesis of CIN. 相似文献