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AIM: To investigate the contribution of angiotensin-converting enzyme inhibitor (ACEI) to the regulation of calpain system in infarcted myocardium. METHODS: Rat myocardial infarction (MI) model was established by permanent ligation of the left coronary artery. The treatment with the ACEI inhibitor rampril (1 mg·kg-1·d-1) was started 7 days prior to surgery. On day 1, 3, 7 and 14 after MI, protein levels of calpainⅠ, Ⅱ and calpastatin were determined in left ventricular free wall (LVFW), interventricular septum (IS) and right ventricule. RESULTS: CalpainⅠprotein level was increased in IS 14 d post MI, whereas the protein level of calpainⅡ was maximally increased in LVFW 3 d post MI. Rampril decreased protein up-regulation of calpainⅠ and Ⅱ, and reduced infarct size and interstitial fibrosis. Calpastatin protein expression was not affected by ACEI. CONCLUSIONS: CalpainⅠ is involved in cardiac remodelling in the late and calpainⅡ contributes to cardiac tissue damage in the early phase of MI. The heart protective effect of ACEI may be related to the inhibition of calpain system in the pathogenesis of myocardial infarction.     

板栗优良新品种‘黄棚’

 ‘黄棚’是20世纪90年代初从海拔300 m以上的山地栗园选出的实生变异优株。经多年高接鉴定和丰产栽培试验表明, 该品种早实, 丰产, 抗旱, 耐瘠薄, 品质优良, 为中早熟、适应范围广的炒食型板栗新品种。     

高效阴离子交换色谱法测定毛头鬼伞多糖中的单糖组成

本文采用高效阴离子交换色谱—脉冲安培检测器（HAPEC-PAD），建立了一种测定多糖中单糖比例的方法。以NaOH为淋洗液、CarboPac^TM A20预处理柱，CarboPac^TMPA20分离柱，金工作电极，Ag/AgCl参比电极，8种自然界中常见单糖标准品做混合标样，探索方法可行性。在淋洗液浓度为2．5mmol／L时，各种单糖组分得到有效分离，其线性和重现性均良好并在此基础上测定了毛头鬼伞多糖中单糖比例；与传统的方法相比，此方法具有前处理简单、灵敏度高、节省时间和试剂等优点。     

冬春茬日光温室苦瓜主要光合特性研究

冬春茬日光温室苦瓜的主要光合作用特性,不同叶位间净光合速率明显不同,幼叶和老叶的光合能力较低,健壮叶片净光合速率最高.净光合速率日变化表现为双峰曲线,有明显的"午休"现象.中午空气湿度低,叶表蒸气压亏缺急剧升高造成气孔导度下降是造成光合"午休"的主要原因.温度明显影响光合作用,苦瓜光合作用最适温度为23℃～34℃.     

红星苹果和肥城桃果实软化的酶学基础

以红星苹果穴Malusdomesticacv.Starking雪和肥城桃穴Prunuspersicacv.Feicheng雪果实为试材,结合乙烯利和1-甲基环丙烯(1-MCP)处理,分别研究了2者软化的酶学基础,并就其差别作了探讨。结果表明:红星果实软化中起主要作用的酶为内切和外切多聚半乳糖醛酸酶(endo-和exo-PG);肥城桃果实软化的前20d则以纤维素酶(EGase)为主,20d后软化以endo-PG为主。研究同时发现,脂氧合酶(LOX)在这2种果实软化的启动阶段都起了明显的促进作用。1-MCP通过对红星果实中PG、肥城桃果实贮藏前期EGase和后期endo-PG活性的抑制,以及对2者软化启动阶段LOX活性的抑制,延缓了果实硬度的下降。     

西瓜核型雄性不育两用系POD同工酶研究

用PAGE(聚丙烯酰胺凝胶)电泳技术,研究了西瓜核型雄性不育两用系不同器官的POD(过氧化物酶)同工酶酶谱差异。试验结果表明,不育株与可育株主要差异在雄花蕾,表现为不育株较可育株多2条活性强的酶带;其他器官差异小,仅表现为酶谱带强弱不同。并对POD同工酶与雄性不育性的相关性进行了研究。     

吉林省荒地资源生态适宜性评价

本文从吉林省荒地资源的实际出发 ,在荒地资源生态适宜性评价基本理论的指导下 ,采用地理信息系统方法 ,对吉林省荒地资源进行了生态适宜性评价。通过空间数据和属性数据的融合、分析、处理 ,可以直观获得荒地资源的生态适宜度等级 ,从而提高评价效率 ,为荒地资源的合理开发利用提供科学依据。     

新疆石河子地区沙漠增温效应对绿洲农业影响的研究

由于沙漠 ,绿洲 ,空气的比热等热性能不同 ,形成沙漠夏季温度明显高于绿洲 ,进而对绿洲地区增温 ,称沙漠增温效应。石河子地区 1 0 0 km范围内沙漠对绿洲的增温平均 1 .76℃ ( 1～ 2℃ ) ;根据绿洲距沙漠的远近与气温间存在的线性关系 ,回归方程为 :y=2 7.5 2 97- 0 .0 335 x。 r=- 0 .881 3*。沙漠增温效应对绿洲农业有多方面作用 ,形成夏季富照高温、秋季大日较差以及促成棉花在夏季的“冷害”等 ,深刻地影响绿洲特色农业、栽培技术及人们生活     

Association of polymorphism of Fc receptor γ chain gene at position-29 in promoter with the susceptibility to systemic lupus erythematosus in southern Chinese

AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P＜0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P＞0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis.     

The mechanism of neuronal injury and repair after focal cerebral ischemia

AIM:To explore the mechanism of neuronal injury and repair by investigating the expression of caspase-3 and apurinic/apyrimidinic endonuclease (APE/Ref-1) after focal cerebral ischemia. METHODS:A model of middle cerebral artery occlusion in rats was performed. The expression of caspase-3P₂₀ and APE/Ref-1 was examined by immunohistochemistry staining, TUNEL was applied to detected DNA damage, and double labeling with TUNEL and APE/Ref-1 was used to determine the relationship between APE/Ref-1 and DNA damage. RESULTS:The active subunit P₂₀ of caspase-3 was predominantly expressed within ischemic penumbra. The peak time of caspase-3P₂₀ positive cells preceded the appearance of TUNEL. With aggravation of cerebral ischemia, APE/Ref-1 immunoreactive cells in penumbra were significantly decreased. CONCLUSION:The activation of caspase enzymatic cascade following cerebral ischemia leads to degradation in DNA, meanwhile, decrease in DNA repair molecules or the failure of DNA repair may deteriorate the course.