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61.
Cytochrome P450 monoxygenase converts arachionic acid to four epoxyeicosatrienoic acid regiosomes: 5, 6-EET (epoxyeicosatrienoic acid); 8, 9-EET; 11, 12-EET and 14, 15-EET. Recent studies show that EETs are involved in signal transduction. EETs open Ca2+-sensit ive K+ channel and inhibit Na+ channel, Ca2+-sensitive Cl- channel and so on. What is more, EETs have been demonstrated to activate PP60c-src and initiate a tyrosine kinase cascade that mediates mitogenic effects. 相似文献
62.
AIM: To investigate the effect of berberine (Ber) on the activation and proliferation of T lymphocytes and its mechanism of action. METHODS: Whole peripheral blood from normal subjects was stimulated with phytohemagglutinin (PHA) or phorbol ester (PDB) plus ionomycin (Ion) and the expression levels of CD69 and CD25 were evaluated with flow cytometry after the staining with appropriate fluorescent monoclonal antibody. The distribution of cell cycles was analyzed by propidium iodide staining and dead cells by 7-aminoactinomycin live staining. RESULTS: 100 μmol/L and 50 μmol/L of Ber had significant inhibition of the expression of CD69 on T cells stimulated with PDB plus Ion or PHA, while effect of 25 μmol/L Ber was not significant. And as time of action extended, the extent of inhibition decreased. For the expression of CD25, Ber at the concentrations as above all exerted significant inhibitory effect in a dose-dependent manner. Moreover, Ber could block lymphocytes cell cycle progression from G0/G1 phase to S and G2/M phase without phase specificity. Besides, live staining analysis revealed that Ber did not have significant cytotoxicity on lymphocytes. CONCLUSIONS: Ber significantly inhibits the expression of early and mid activation antigens of T cells and also blocks the progression of lymphocytes cell cycles. These results suggest that Ber exerts immunosuppression effect through inhibiting the activation and proliferation of T cells. 相似文献
63.
AIM: To study the electrophysiological characteristics of ion channels of stem cell derived cardiomyocytes(SCDC) of mouse. METHODS: Embryonic stem cells of D3 line(ES-D3) were cultured on the MEF feeder layer with BRL conditioned medium, and fetal mouse heart cells(FMHC)were cultured in vitro. Then ES-D3 cells were induced to differentiate into many kinds of cells. SCDC were harvested on day 12 after differentiation initiating and identified by electro-microscope and immunocytochemistry. SCDC and FMHC were prepared for the patch-clamp research. Sodium and calcium currents together were elicited and compared between SCDC and FMHC. RESULTS: The current characteristics of sodium and calcium channels of SCDC were very similar to FMHC. CONCLUSION: The functional expression of ion channels occurred during ES-D3 cells differentiation and the electrophysiological characteristics of sodium and calcium channels of SCDC are very similar to FMHC. 相似文献
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研究了两种β-环糊精的衍生物甲基-β-环糊精(MCD)和羟丙基-β-环糊精(HPCD)对甲基对硫磷的增溶作用和对紫外光降解的影响。结果表明:MCD和HPCD能增强甲基对硫磷的水溶性,在25℃下,20 g/L的MCD和HPCD溶液中,甲基对硫磷溶解度比在纯水中分别提高了21.91和17.92倍;另外,3 g/L、6 g/L MCD和HPCD分别处理的甲基对硫磷,其光降解速率分别加快了4.87~6.85倍。增溶作用和光敏效应主要是由于MCD和HPCD与甲基对硫磷形成包合物引起的。 相似文献
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粳稻品种抗稻瘟病性与其生物学性状的相关关系研究 总被引:2,自引:0,他引:2
对342份粳稻材料进行抗稻瘟病性(抗叶瘟、穗颈瘟)与其生物学性状(株高、穗长、每穗粒数、分蘖率、单株有效分蘖数、空秕率、着粒密度,以及成熟期等)相关关系研究,结果不同品种(系)间存在着一定差异。抗叶瘟性与株高、穗长、每穗粒数、单株有效分蘖数、着粒密度呈显著正相关,相关系数依次为:0.409、0.491、0.426、0.657、0.471;与空秕率呈显著负相关,相关系数为-0.591。抗穗颈瘟性与株高、每穗粒数、单株有效分蘖数呈显著正相关,相关系数分别为:0.717、0.462、0.362;与空秕率、分蘖率、着粒密度呈显著负相关,相关系数依次为:-0.343、-0.332、-0.476。试验表明,株高、单株有效分蘖率、每穗粒数、空秕率等性状,可做为主要标记,在粳稻品种(系)抗稻瘟病性选鉴上应用 相似文献
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