Characteristics of 18F-FDG and 18F-FDOPA PET in an 8-year-old neutered male Yorkshire Terrier dog with glioma: long-term chemotherapy using hydroxyurea plus imatinib with prednisolone and immunoreactivity for PDGFR-β and LAT1

An 8-year-old neutered male Yorkshire Terrier dog presented with head pressing, vestibular ataxia, neck tenderness, and no oculocephalic reflex. A demarcated lesion in the pons was identified on MRI. The patient was tentatively diagnosed with a glioma and was treated with hydroxyurea plus imatinib and prednisolone. After 30 days of therapeutic treatment, the patient showed a clear improvement in neurological signs, which lasted for 1117 days. On day 569 after the initiation of treatment, ¹⁸F-fluorodeoxyglucose (FDG)-positron emission tomography (PET) was performed with no significant findings on visual analysis. The average and maximal standardized uptake values (SUVs) were 1.92 and 2.29, respectively. The tumor-to-normal-tissue (T/N) ratio was 0.97. The first evidence of clinical deterioration was noticed on day 1147. On day 1155, 3,4-dihydroxy-6-[¹⁸F]-fluoro-l-phenylalanine (¹⁸F-FDOPA)-PET was performed. High uptake of ¹⁸F-FDOPA was observed in the intracranial lesion. The mean and maximal SUVs of the tumor were 1.59 and 2.29, respectively. The T/N ratio was 2.22. The patient was euthanized on day 1155 and histopathologic evaluations confirmed glioma (astrocytoma). This case shows that chemotherapy with hydroxyurea plus imatinib may be considered in the treatment of canine glioma. Furthermore, this is the first case describing the application of ¹⁸F-FDG and ¹⁸F-FDOPA in a dog with glioma.     

一起野禽H5N8亚型禽流感疫情的处置与思考

2021年1月,江苏省连云港市云台山景区云台街道西隅水库发生一起疑似野禽H5N8亚型禽流感疫情,共17只鸳鸯、大雁感染死亡。江苏省动物疫病预防控制中心迅速组织相关人员,指导连云港市按照国家相关方案和规范要求,开展现场调查、实验室诊断和应急处置工作。经江苏省动物疫病预防控制中心初诊,国家禽流感参考实验室确诊,诊断为H5N8亚型高致病性禽流感疫情。同时,指导当地政府划定疫点、疫区和受威胁区,封锁相关区域,开展应急监测、紧急流行病学调查、全面消毒等应急处置工作。经果断处置,疫情得到了有效控制,未造成扩散和蔓延。本次疫情提示：野禽疫情的早期发现和及时控制对于降低疫情扩散风险至关重要;林业和农业农村部门的沟通协作要继续加强,日常的禽流感监测力度需要进一步加大,家禽的禽流感疫苗免疫要更加科学,群众的禽流感防控意识要持续提高。     

‘清水’苜蓿与‘WL168’杂交后代的表型特征及生理特性

本研究以‘清水’紫花苜蓿（Medicago sativa L.‘Qingshui’）与‘WL168’紫花苜蓿（Medicago sativa L.‘WL168’）的杂交后代选育系RSA-01,RSA-02和RSA-03为研究对象,于种植第5年对其表型特征及生理特性进行观测分析。结果表明：RSA-01,RSA-02地上生物量显著低于‘WL168’（P<0.05）,而RSA-03显著高于‘清水’;RSA-02,RSA-03茎粗显著高于‘清水’和‘WL168’,而茎叶比均低于‘清水’;各形态指标中,茎粗变异系数最小,且RSA-03值最小,为2.17%;通径分析表明,RSA-01,RSA-02和RSA-03的茎叶比、茎粗与其地上生物量的直接通径系数分别为-0.649和0.709,-0.700和0.422,-0.858和0.189,茎叶比、茎粗是影响地上生物量的关键因素;RSA-01,RSA-03的过氧化物酶活性显著低于‘清水’和‘WL168’。与亲本‘清水’相比,RSA-03的地上生物量和茎粗都具有显著的杂种优势,但其抗逆性减弱,该研究结果为后期产量改良及抗逆性研究提供理论依据。     

Rapidly quantitative detection of Nosema ceranae in honeybees using ultra-rapid real-time quantitative PCR

BackgroundThe microsporidian parasite Nosema ceranae is a global problem in honeybee populations and is known to cause winter mortality. A sensitive and rapid tool for stable quantitative detection is necessary to establish further research related to the diagnosis, prevention, and treatment of this pathogen.ObjectivesThe present study aimed to develop a quantitative method that incorporates ultra-rapid real-time quantitative polymerase chain reaction (UR-qPCR) for the rapid enumeration of N. ceranae in infected bees.MethodsA procedure for UR-qPCR detection of N. ceranae was developed, and the advantages of molecular detection were evaluated in comparison with microscopic enumeration.ResultsUR-qPCR was more sensitive than microscopic enumeration for detecting two copies of N. ceranae DNA and 24 spores per bee. Meanwhile, the limit of detection by microscopy was 2.40 × 10⁴ spores/bee, and the stable detection level was ≥ 2.40 × 10⁵ spores/bee. The results of N. ceranae calculations from the infected honeybees and purified spores by UR-qPCR showed that the DNA copy number was approximately 8-fold higher than the spore count. Additionally, honeybees infected with N. ceranae with 2.74 × 10⁴ copies of N. ceranae DNA were incapable of detection by microscopy. The results of quantitative analysis using UR-qPCR were accomplished within 20 min.ConclusionsUR-qPCR is expected to be the most rapid molecular method for Nosema detection and has been developed for diagnosing nosemosis at low levels of infection.     

内蒙古贺兰山马鹿的种群数量及种群结构

为了掌握内蒙古贺兰山国家级自然保护区内马鹿(Cervus elaphus)的种群数量和种群结构,维护内蒙古贺兰山的生态平衡。于2017年11—12月,2018年4—6月、11—12月,2019年4—6月,在内蒙古贺兰山国家级自然保护区内,利用样线法对保护区内的马鹿进行调查,采用Distance R进行数据分析,估测种群数量和种群密度,并对种群结构进行探讨。研究发现,马鹿在2018年冬季种群数量最高约为2452(1678—3578)只,种群密度为3.705(2.539—5.048)只/km²,遇见率为1.943只/km。遇见率年际间变化不明显(F=0.12,P=0.986);混合群出现的次数最高,雄性群出现的次数最低,不同集群类型在不同季节的差异极显著(P<0.001);群大小在不同季节的差异不显著(P=0.132);雌雄比在不同季节中没有太大变化。     

Starch digestion rates in multiple samples of commonly used feed grains in diets for broiler chickens

In this study the starch digestion rates in broiler chickens from 18 samples of 5 commonly used feed grains (sorghum, wheat, maize, barley, triticale) were determined. The methodology to determine starch digestion rates in poultry is detailed herein. Starch digestion rates were not significantly different (P = 0.128) across the 18 feed grains, which reflects the wide variations that were observed within a given feedstuff. Nevertheless, starch digestion rates in broiler chickens offered wheat-based diets were significantly more rapid by 56.0% (0.117 versus 0.075 min⁻¹; P = 0.012) than their sorghum-based counterparts on the basis of a pair-wise comparison. In descending order, the following starch digestion rates were observed: wheat (0.117 min⁻¹), barley (0.104 min⁻¹), triticale (0.093 min⁻¹), maize (0.086 min⁻¹), sorghum (0.075 min⁻¹). The implications of these findings are discussed as they almost certainly have implications for poultry nutrition and the development of reduced crude protein diets for broiler chickens.     

小型多功能底盘稻麦割晒机研究

针对贵州山区田块小而分散,提出设计稻麦割晒机时应采用适应多种田间作业需要的通用底盘。小型多功能底盘主要由多功能底盘、发动机、动力传动系统、操作手柄及前后连接支架组成,多功能底盘上安装前后连接支架,根据田间农作物作业需要,与通用底盘快速挂接,可完成旋耕整地、开沟起垄、播种施肥、灌水施药及稻麦割晒等作业。该底盘可构建多功能作业机组,扩大机具使用范围和利用率,解决丘陵山地小田块机械化收割问题。     

烟草田间起垄机作业适应性研究

对开沟式和盒式的1WG-6.3型自走式小型起垄机进行田间试验,并对其作业效益、作业效率及作业效果等指标进行测量及性能分析对比。结果表明:盒式起垄机每公顷平均油耗较低,作业效率较高,稳定性好,起垄效果较好,能满足烤烟种植起垄效果的农艺要求;开沟式起垄机能满足烤烟种植垄底宽、垄高的农艺要求,但不能满足烤烟种植垄基宽的农艺要求,且每公顷平均油耗较高,作业效率较低,稳定性较差,垄形一般。因此,盒式起垄机更能适应烟草田间起垄作业。     

ZigBee和线阵CCD技术在西藏智能温室中的应用

利用西藏地区太阳能丰富的优势,采用太阳能资源为主要供能方式,为智能温室系统构建基于Zig Bee协议的无线传感网络和nRF2401无线传感器的双层无线通信网络,以减少西藏地区有线布线成本和解决温室群控制问题。同时,在传统智能温室中结合线阵CCD技术,为智能温室系统提供实时、直观的作物生长状况数据,有效提高对温室内环境因素的监控效率及温室智能自动化控制程度,以满足现代化高效节约型农业的发展形势需求,以及西藏地区特定的地理环境对于农业自动化的需求。     

Cloning and Sequence Analysis of HA and NA Genes of One Strain of H6N6 Subtype Avian Influenza Virus Isolated from Guizhou Province

To investigate the epidemic situation of H6N6 subtype avian influenza virus (AIV) in Guizhou province,A/duck/Guizhou/013/2014 was isolated from Sansui duck in live poultry market of Guizhou in 2014,the hemagglutinin (HA) and neuraminidase (NA) genes of DK/GZ/14 were subjected to clone and sequence analysis.The results showed that HA gene had the highest nucleotide homologies (97.5%) with the duck-origin H6N6 subtype AIV isolated from Eastern China in 2009,and the strains of HA gene proteolytic cleavage sites was P-Q-I-E-T-R-G,which accordeol with the molecular characteristic of low pathogenic AIV (LPAIV).However,NA gene of A/duck/Guizhou/013/2014 had the highest nucleotide homologies (98.2%) with the duck-origin H6N6 subtype AIV isolated from Fujian in 2007.The phylogenetic tree showed that A/duck/Guizhou/013/2014 and Hunan strains located in the same branch,while three duck-origin H6N6 subtype AIV isolated from Guizhou in 2007 and A/duck/Guizhou/013/2014 located in the different branch for HA and NA genes in genetic evolution,which suggested that A/duck/Guizhou/013/2014 was far with the local H6N6 subtype.The results also clearly indicated that duck-origin H6N6 subtype AIV had genetic diversity in duck population in Guizhou.