草生栽培对(木奈)园温湿度和果实品质的影响

1999- 2 0 0 1年进行了草生栽培对园温湿度和果实品质影响的试验。结果表明 :草生栽培可以有效地改善果园生态环境 ,降低久旱暴雨后的裂果率 ,有利于提高果的商品质量和经济效益。     

Protective effect of onychin on the human umbilical vein endothelial cells injured by menadione

AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO₂^-／NO₃^- concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO₂^-／NO₃^- concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway.     

Effect of cocaine on caspase-3 in myocardiac cells of male rats in different age

AIM: To study the effect of cocaine on caspase-3 in myocardiac cells of male rats in different age. METHODS: Three-week-old(n=16), six-week-old(n=16) and twelve-week-old (n=16) male Sprague-Dawley rats were all divided into control groups and experiment groups randomly, each group had eight animals, experiment groups were given cocaine hydrochloride (15 mg·kg^-1 body weight) subcutaneously daily for four weeks. The ratio of heart weight to body weight (HW/BW, mg/g) were measured. DNA fragmentation of myocardia cells was determined by gel electrophoresis, and caspase-3 activity in myocardia cells was tested by flow cytometry (FCM). RESULTS: In three experiment groups, the DNA isolated from myocardial cells displayed clear ladder pattern. The HW/BW and the caspase-3 activity were increased significantly than those of control groups (P<0.01). CONCLUSIONS: Cocaine induced apoptosis in rat myocardial cells. The increase in caspase-3 activity may be the one of important pathways related to the induction of apoptosis.     

额尔齐斯河流域生态系统格局及变化

本文首先对额尔齐斯河流域气候，地貌，水文等环境因子特征进行了评述，进而阐述了流域生态系统的特征和格局以及在自然和人为干扰作用下的变化。分析结果表明在自然干扰作用下流域生态系统的格局与流域水文情势变化相适应，处于准稳定变化状态，而在人为对流域水文情势扰动下，流域生态系统的格局间接地受到影响。从而产生新的格局，其作用的强度，持久性和生态系统的格局变化程度过错大于大于自然干扰作用下的变化。引额调水工程对额尔齐斯河流域河谷生态系统内部单元格局变化具有重要影响。     

腐败组织中毒鼠磷的GC／NPD、GC／ECD检验

本文初步建立了一套混合溶剂提取、中性层析氧化铝小柱净化及GC／ECD和GC／NPD分析的腐败生物样材中毒鼠磷的检验方法。GC／ECD的检出限为1ng;GC/NPD的检出限为20ng。     

巴旦杏在甘肃河西地区引种培育问题的探讨

该文通过分析巴旦杏在甘肃河西地区引种培育过程中出现的问题,总结出用容器育苗和大田开沟点播相结合来培育接穗,以山桃为砧木嫁接巴旦杏的引种驯化技术.结果表明,容器培育接穗比直接大田点播出苗率、成活率分别提高了10%和15%;巴旦杏与山桃嫁接表现出极好的亲和性,嫁接成活率为90%.     

长豇豆品种同功酶的遗传多样性分析

用电泳技术分析了长豇豆21个品种种苗的过氧化氢酶(CAT)和淀粉酶(AMY)的同功酶酶谱.从中分析了3个表现出品种之间多样性的位点.根据这些位点上带型的差异,可以区分供试的所有长豇豆品种.图谱上品种之间的差异位点,可以用于资源品种亲缘关系分析和纯度鉴定.     

粉蕉、大蕉和龙牙蕉的AFLP分类研究

 应用AFLP 技术, 对32 个粉蕉、大蕉、龙牙蕉品种进行了遗传多样性分析及分类研究。在分子水平上, 可将供试品种聚为6 个独立群体, 其中包括3 个分别由单一品种组成的群体(群体Ⅳ: 酸大蕉;群体Ⅴ: 孟变; 群体Ⅵ: 千旁培) ; 可以将群体Ⅰ (大蕉) 和群体Ⅱ (粉蕉) 分别划分为3 个品种群, 将群体Ⅲ (龙牙蕉) 划分为2个品种群。参照Simmonds 的形态学分类方法, 研究结果显示粉蕉、大蕉以及龙牙蕉各个品种之间存在丰富的遗传多样性, 将其划分为AAB 或ABB 等类型, 不能反映品种间的亲缘关系; 粉蕉与大蕉两个类群之间遗传距离差异明显, 将其简单的归为ABB 类型是不妥当的。     

灰树花不同菌株液体深层培养蛋白质含量的测定

在液体培养条件下测定了发酵液中5个灰树花菌株蛋白质含量的变化。结果表明，接种后第6天发酵液中蛋白质含量最高，Gf961菌株发酵液中的蛋白质含量为0.58%，Gf962菌株发酵液中的蛋白质含量最低，为0.30%，随着培养时间的延长，发酵液中蛋白质含量逐渐下降，接种后第9天最低，随后以呈上升趋势。但上升的幅度较小。试验为确定从发酵液中提取蛋白质的最佳时期提供了一定的理论依据。     

苹果粗皮病与锰含量的关系

对胶东半岛部分发生苹果粗皮病(IBN)的果园的土壤和植株进行了分析测定,结果表明:发生苹果粗皮病的果园土壤有效锰含量较高,而且粗皮病严重果园高于粗皮病较轻果园。发生粗皮病的苹果树花器官中锰含量较高,粗皮病最严重的姜格庄果园苹果树花器官中锰平均含量比观水镇高出139.28mg/kg,比季家埠重病园和轻病园分别高122.34和182.19mg/kg。从4月到10月,苹果粗皮病逐渐加重,叶片中锰含量逐步增高。10月份姜格庄果园最高锰含量达1717.85mg/kg,平均锰含量达1119.69mg/kg,而粗皮病较轻的季家埠果园则仅306.03mg/kg,前者比后者高265.88%。对不同部位锰含量测定表明,锰含量在叶片中最高,其次是韧皮部,木质部最低。表明胶东半岛苹果粗皮病的发生是因为锰过量。可能是土壤锰含量高,pH值低,降雨多,施肥不合理等,造成土壤锰有效性增加.导致树体吸收过量而出现粗皮病害。