Acorn hoarding by the field mouse,Apodemus speciosus Temminck (Rodentia: Muridae)

We studied population changes of the field mouse,Apodemus speciosus Temminck, by live trapping in a mixed stand of cypress and broad-leaved trees at Tama Forest Science Garden during the period from October 1991 to March 1994. We also used radiotelemetry to investigate acorn hoarding. The number of mice captured in 1992 was three to ten times higher than that in either 1991 or 1993. The home ranges of resident mice overlapped greatly, but a path and differences in the vegetation structure may have affected the home range of individuals. From June 1992 to April 1993, mice actively transported and hoarded acrons. Individuals hoarded 70% of the acorns in their home ranges within one day after release. This species was a typical scatter-hoarder and hoarded a single acorn at each cache site. Mice buried acrons in the soil at the first hoarding, but recovered and carried them into their nests later. Although more than 50% of the acorns were buried 0–5 cm deep, where the condition for germination seemed to be good, mice recovered and ate all cached acorns. Mice relocated acorns farther from the original food station as they repeated hoarding. Mice transported acorns an average of about 15–18 m (range: 2.2–49.8 m) before they ate them. From May 1993 to March 1994, the hoarding behavior of mice was not active. Most acorns were left at food stations or cache sites for more than three months. Acorn hoarding byA. speciosus probably contributes little to the dispersal and regeneration of acorn-producing trees in years with a high rodent density.     

Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)

Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.     

Evaluation of the management practice for controlling herbicide runoff from paddy fields using intermittent and spillover-irrigation schemes

Two water management practices, an intermittent irrigation scheme using automatic irrigation system (AI) and a spillover-irrigation scheme (SI), were compared for the fate and transport of commonly used herbicides, mefenacet (MF) and bensulfuron-methyl (BSM) in experimental paddy plots. Maximum mefenacet concentrations in paddy water were 660 and 540 μg L⁻¹ for AI and SI plot, respectively. The corresponding values for bensulfuron-methyl were 46.0 and 42.0 μg L⁻¹. Dissipation of the herbicides in paddy water appeared to follow the first-order kinetics with half-lives (DT₅₀) of 1.9–4.5 days and DT₉₀ (90% mass dissipation) of 7.8–11.3 days. The AI plot had no surface drainage, hence no herbicide was lost through paddy-water discharge. However, SI plot lost about 38 and 49% of applied mefenacet and bensulfuron-methyl, respectively. The intermittent irrigation scheme using automatic irrigation system with a high drainage gate was recommended to be a best management practice for controlling the herbicide losses from paddy fields. The paddy field managed by spillover-irrigation scheme may cause significant water and herbicide losses depending on the volume of irrigation and precipitation. The water holding period after herbicide application was suggested to be at least 10 days according to the DT₉₀ index.     

Natal dispersal of hazel grouse Bonasa bonasia in relation to habitat in a temperate forest of South Korea

Natal dispersal and habitat selection of hazel grouse Bonasa bonasia juveniles were assessed by radio tracking in a temperate forest of South Korea during June 2003–November 2006. The birds used in the dispersal analysis (n = 43), 88% of birds dispersed. The average dispersal of hazel grouse from the natal area was 2231 ± 494 m (mean ± SD); range 134–6267 m. There were no significant differences in dispersal rate and distance between sexes. Hazel grouse’ habitat use differed from random use of available natal dispersals. In this natal dispersal scale of habitat selection, natural deciduous forest ranked highest, followed by mixed forest, coniferous plantation, deciduous plantation and others (rock and bare lands). We found that in general, the hazel grouse is an active disperser. Knowledge of aspects of habitat use that are relevant to dispersal may provide a better assessment of the fitness effects of dispersal in proximate and ultimate terms.     

Optimum feeding rates in juvenile olive flounder,Paralichthys olivaceus,at the optimum rearing temperature

Two feeding trials were carried out to determine the optimum feeding rates in juvenile olive flounder, Paralichthys olivaceus, at the optimum rearing temperature. Fish averaging 5.0 ± 0.11 g (mean ± SD) in experiment 1 and 20.2 ± 0.54 g (mean ± SD) in experiment 2 were fed a commercial diet at the feeding rates of 0%, 3.0%, 4.0%, 4.25%, 4.5% and 4.75% body weight (BW) day^?1 and satiation (5.52% BW day^?1) in experiment 1 and 0%, 1.0%, 2.0%, 3.0% and 3.5% BW day^?1 and satiation (4.12% BW day^?1) in experiment 2 at 20 ± 1 °C. Both feeding trials lasted for 2 weeks. Results from experiment 1 indicated that weight gain (WG) and specific growth rate (SGR) of fish fed to satiation were significantly higher than those of fish fed at other feeding rates while feed efficiency (FE) and protein efficiency ratio (PER) of fish fed at 4.25% BW day^?1 were significantly higher than those of fish fed to satiation and fish fed at 3.0% BW day^?1 (P < 0.05). In experiment 2 WG, SGR and PER leveled out after the feeding rate of 3.5% BW day^?1 whereas FE reached a plateau at 3.0% BW day^?1. anova of FE indicated that the optimum feeding rates in 5.0 and 20 g juvenile olive flounder could be 4.25% and 3.0% BW day^?1, respectively. Broken line analysis of WG suggested the optimum feeding rates of 5.17% and 3.47% BW day^?1 in 5.0 and 20 g fish, respectively. Therefore, these results indicated that the optimum feeding rates could be >4.25 but <5.17% BW day^?1 for 5.0 g, and it could be >3.0 but <3.47% BW day^?1 for 20 g size of juvenile olive flounder at the optimum rearing temperature.     

Culture-based fisheries in small reservoirs in northern Vietnam: effect of stocking density and species combinations

This study was conducted in 20 reservoirs, ranging in size from 4 to 30 ha, in the mountainous, northern region of Vietnam, in ThaiNguyen and YenBai provinces, over two growth cycles in 2002/03 and 2003/04. The reservoirs are leased by farmers for fishery activities from the provincial administration, and the trials were managed by the lessee farmers. Three species combinations in ratios (by fingerling weight) of grass carp: silver carp: bighead carp: common carp: mrigal 1:2:1:1:3 (A), 1:3:1:1:2 (B) and 1:2:1:1:2 (C) were used as seed stock. The overall mean yield of stocked fish in 2002/03 and 2003/04 growth cycles in reservoirs in ThaiNguyen and YenBai provinces was 165 (±21) and 190 (39), and 287 (±22) and 325 (±24) kg ha^?1 respectively. The yield in reservoirs in both provinces, in both growth cycles and irrespective of the species combinations, increased in relation to stocking density. In reservoirs in ThaiNguyen Province, the species combination B gave the lowest yield (both growth cycles and overall), and differed significantly (P<0.05) from combinations A and C. The stocking efficiency (ratio of the yield of stocked fish in kg ha^?1 to the weight of the stocked fish in kg ha^?1) in reservoirs in ThaiNguyen Province ranged from 2.9 to 5.1 over the two growth cycles and that in YenBai from 2.8 to 3.9. There was no discernible trend between growth cycles and/or between species combinations. The major cost incurred was for fingerling procurement. In all instances, a net profit was accrued. The mean (±standard error (SE)) net profit ha^?1 (in 10³VN dong; approximately 15 500 VND=1 US$) was 885 (±270) and 864 (±214), and 1322 (±176) and 1600 (±150) for the growth cycles 2002/03 and 2003/04 for reservoirs in ThaiNguyen and YenBai provinces respectively. Between the two growth cycles, the net profit increased in eight and three reservoirs from YenBai and ThaiNguyen, respectively, the maximum increase being recorded in Khuan Gio (165%) and Dong Man (39%) reservoirs.     

Protective Effects of Platycodon grandiflorum Aqueous Extract on Thioacetamide-induced Fulminant Hepatic Failure in Mice

The aim of the present study was to evaluate the protective activity of aqueous extract from Platycodon grandiflorum (BC703) on thioacetamide (TA)-induced hepatotoxicity in mice. We found that BC703 significantly decreased mortality and the change in serum transaminase following TA administration. The group treated with BC703 at doses of 1, 5, and 10 mg/kg produced significant hepatoprotective effects against TA-induced liver damage by decreasing the activities of serum enzymes, nitric oxide and lipid peroxidation in dose-dependent manners. Histopathological studies further substantiated the protective effect of BC703. These results show the hepatoprotective activity of aqueous extract from Platycodon grandiflorum on thioacetamide-induced fulminant hepatic failure.     

Estimation of individual leaf area,fresh weight,and dry weight of hydroponically grown cucumbers (Cucumis sativus L.) using leaf length,width, and SPAD value

Non-destructive and mathematical approaches of modeling can be very convenient and useful for plant growth estimation. To predict individual leaf area, fresh weight, and dry weight of a cucumber (Cucumis sativus L.), models were developed using leaf length, leaf width, SPAD value, and different combinations of these variables. Eight regression equations, commonly used for developing growth models, were compared for accuracy and adaptability. The three nonlinear models developed were as follows: individual leaf area (LA) = −210.61 + 13.358W + 0.5356LW (R² = 0.980^***), fresh weight (FW) = −2.72 + 0.0135LW + 0.00022LWS (R² = 0.956^***), and dry weight (DW) = 0.25 − 0.00102LS + 0.000077LWS (R² = 0.956^***), where L is the leaf length, W the leaf width, S the SPAD value, and LWS = L × W × S. For validation of the model, estimated values for individual leaf area, fresh weight, and dry weight showed strong agreement with the measured values, respectively. Leaf dry weight, especially, was estimated with a higher degree of accuracy through the use of a SPAD value, as well as leaf length and width. Therefore, it is concluded that models presented herein may be useful for the estimation of the individual leaf area, fresh weight, and dry weight of a cucumber with a high degree of accuracy.     

Simulation of mefenacet concentrations in paddy fields by an improved PCPF-1 model

An improved simulation model (PCPF-1) has been evaluated for the prediction of the fate of mefenacet in an experimental paddy field. This model simulates the fate and transport of pesticide in paddy water and the top 1 cm of paddy soil. Observed concentrations of mefenacet in the paddy water and the surface soil exponentially decreased from their maximum concentrations of 0.70 mg litre(-1) and 11.3 mg kg(-1), respectively. Predicted mefenacet concentrations both in the water and surface soil were in excellent agreement with those measured during the first 2 weeks after herbicide application, but concentrations in paddy water were appreciably overestimated thereafter. The model simulated mefenacet losses through runoff, percolation and degradation to be respectively 41.9%, 6.4% and 57.3% of applied, and the mass balance error was about -6%. The model simulation implied that drainage and seepage control, especially shortly after application when herbicide concentrations are high, is essential for preventing pesticide losses from paddy fields. In focusing on pesticide concentrations in this early period the PCPF-1 model can be a beneficial tool for risk assessment of pesticide losses and in the evaluation of agricultural management for reducing pesticide pollution associated with paddy rice production.