Study on Proliferation and Apoptosis of EBL Cells Induced by P48 Protein of Mycoplasma bovis

The aim of this study was to investigate the effect of P48 protein on proliferation and apoptosis of embryonic bovine lung (EBL) cells.In this study,samples which co-incubated with P48 protein and EBL cells in different concentrations at different time points were collected,the proliferation rate of the cells was detected by MTT method,and the changes in the nuclear morphology of EBL cells were observed by DAPI staining method.Meanwhile,flow cytometry was used to detect the apoptosis rate of EBL cells induced by P48 protein.Real-time quantitative PCR was used to detect the changes in mRNA level of apoptotic marker genes,and Western blotting tested the Bax and Beclin-1 protein expressions.The results showed that under the condition of 72 h and 10 μg/mL of protein concentration treatment,P48 extremely significantly inhibited EBL cells proliferation (P<0.01),while 0.1 and 0.5 μg/mL protein concentration had no inhibitory effect (P>0.05).The nuclear morphology showed no significant change after protein induction for 12 h,but wrinkled and condensed at 24 h.The nucleus was fragmented,and a sprouted apoptotic body was appeared at 48 and 72 h.Apoptosis related genes expression showed no obvious increase at 2 and 12 h at mRNA level,but gradually increased at 24,48 and 72 h,and it showed a time-dependent manner.Accordingly,the expression of apoptosis marker proteins Bax and Beclin-1 significantly increased.Flow cytometry analysis showed that the apoptosis rate of EBL cells induced by P48 protein was 48.44%.In conclusion,P48 recombinant protein of Mycoplasmas bovis inhibited the proliferation of EBL cells and promoted their apoptosis,which provided reference for revealing the pathogenic mechanism of Mycoplasmas bovis.     

霜冻害对苗圃育苗质量的影响分析

分析了苗圃育苗霜冻害产生的原因,提出预防霜冻害的措施。     

生活垃圾填埋场渗滤液水质变化研究

对生活垃圾渗滤液的水质特性进行了研究,并研究了填埋时间等环境条件对渗滤液水质变化的影响,表明了渗滤液成分的复杂性.     

从我国木材资源的变化看普通刨花板的发展

分析了国内普通刨花板及其成套生产设备的现状及与国际先进水平的主要差距。通过对我国木材资源结构变化状况的论述，阐明了普通刨花板产业在我国的适应性以及发展趋势。介绍了苏福马公司在普通刨花板成套生产线设备发展中的实践及发展目标。     

东北老工业基地振兴与东四盟生态建设

东四盟是东北老工业基地振兴的重要组成部分,是国家重要的后备森林资源基地,是国家林业建设的重点地区,是我国北方地区的生态屏障。东北老工业基地振兴必然带动东四盟市快速发展,东四盟市经济快速发展和生态环境改善必然推动和支持东北老工业基地的长期繁荣。     

浙江省药用植物资源利用现状及开发利用途径

在实地调查的基础上,介绍了浙江省药用植物资源利用现状、分析了资源利用和生产经营过程中存在的问题与不足,指出了浙江省药用植物资源开发利用途径和科研方向.     

萃取柿叶精油的工艺研究

通过单因素试验和正交试验研究了超临界流体萃取柿叶精油的影响因素,并对生产工艺进行了优化设计.试验结果表明,萃取压力、萃取温度、CO2流量、萃取时间对萃取得率的影响程度依次递减.最佳工艺条件为,100 g柿叶在萃取压力18 MPa、萃取温度50 ℃、CO2流量20 kg/h、萃取时间2.5 h,得率为3.1 %.用超临界CO2萃取柿叶精油比水蒸气法蒸馏的柿叶精油得率提高了55 %,萃取物纯度高,有浓郁的柿果香味,色淡黄.     

光照强度对雷竹无性系生长的影响

通过野外实验，对雷竹无性系在不同光照强度下的生长进行了对比分析，结果表明，随着光照强度的降低，雷竹无性系分株数、分株高度、分株基径、无性系根茎总长度、分株根茎长度显著降低，而间隔子长度不断增加；在不同光照条件下，根茎节间长度和分枝角度没有显著变化；随着光照强度的降低，无性系单位长度根茎生物量下降。并对雷竹无性系根茎生长对光照强度的适应对策进行了讨论。     

把林火灾害问题纳入风险管理范畴的探讨

风险管理正在成为全球关注的新学科。森林火灾不可避免,灾害风险不容回避,把林火灾害纳入风险管理范畴,是预防、减少和控制森林火灾的重要手段和措施。文中阐明了林火灾害风险及风险管理的概念和含义,明确了风险判识、风险分析、风险评估和风险控制特别是风险评估的内容与方法。