Impact of serial activation of embryonic pax2 and WT1 genes on transdifferentiation of renal tubular epithelial cells

AIM: To investigate the mechanisms of transdifferentiation of renal tubular epithelial cells by the observation of serial activation of embryonic pax2 and WT1 genes in chronic renal failure model of 5/6 nephrectomized injury. METHODS: Embryo of mice, cultured renal tubule cells and chronic renal failure rat model of 5/6 nephrectomized injury were established. The expressions of paired Box gene (pax2) and Willm’s tumor gene (WT1), as well as α-smooth muscle actin (α-SMA), a phenotype of mesenchymal cells, were detected by RT-PCR and immunohistochemistry techniques. RESULTS: (1) Expressions of pax2 and WT1 mRNA began at 11.5 d and 14 d of embryo and increased gradually, and expression in trace quantity at 2 weeks after birth. Immunohistochemistry analysis revealed that WT1 only expressed in the glomerular podocytes and expressions of pax2 and WT1 in adult renal tubular cells were negative. (2) Deno-expression of pax2 and WT1 in some tubular cells appeared at week 2 and peaked at week 4 in 5/6 nephrectomized rats, both showing a same trend of expression. However, pax2 showed another peak at week 10 afterwards. (3) Re-expressions of pax2 and WT1 in TEC at 0.5 and 24 h after treated with IL-1α (10 μg/L) or AngII (10-9 mol/L) were observed respectively, followed by upregulation of α-SMA expression, and mesenchymal cells characters were shown. The effects were inhibited by Ang II receptor 2 antagonist and WT1 antibody, respectively. CONCLUSION: Adult renal tubule cells acquire re-activation of embryonic pax2 and WT1 genes and phenotypes of mesenchymes when challenge with certain injuries. Deno-expression of pax2 and WT1 genes closely relates with high concentration of bioactive facors, such as AngII and IL-1, which are involved in the mechanisms of renal tubule cells transdifferentiation.     

低温处理对不同油豆角品种幼苗的影响

将7个品种的油豆角进行5℃低温处理,处理7 d,通过对它们的根系活力、脯氨酸含量、丙二醛含量、过氧化物酶活性及叶绿素含量的测定,来比较研究低温处理对不同品种油豆角的影响.研究表明:不同品种的低温反应不同,太空的耐低温性最强,超纯的耐低温性是最弱的,其余品种,介于它们之间.     

棉籽壳不同预处理与平菇子实体中游离棉酚的含量

通过在100 ℃和126 ℃下灭菌及堆积发酵等处理的含87%棉籽壳的培养料来栽培平菇,然后测定平菇子实体中棉酚的含量,结果发现棉酚含量均在国家安全标准(≦0.02%即200 mg/kg)以内,其中以堆积发酵18 d的培养料栽培得到的平菇子实体棉酚含量最低,只有24.00 mg/kg.     

盐碱地低盐水池塘网箱套养漠斑牙鲆技术研究

根据漠斑牙鲆(Paralichthys lethostigma)适广温、广盐和较强的抗逆性等优良生态习性,采用地下卤水、盐碱地渗水,通过合理淡水配兑使其适合漠斑牙鲆生理生长需求,在我国中纬度地区利用盐碱地低盐水养殖过洋、暖温性漠斑牙鲆。2004-2005年,利用盐碱地低盐水池塘虾池设置网箱套养漠斑牙鲆349.5m2,产鱼3.749t,产虾55.065t,其中2005年虾池设置网箱套养漠斑牙鲆306m2,产鱼3.245t,平均10.6kg/m2,平均全长34.0cm,体重527.4g,养殖成活率为90.5%,产虾49.003t,平均408.4kg/亩,平均规格11.9cm,养殖成活率为71.23%。虾池设置网箱套养漠斑牙鲆,可充分利用水体空间,促使池塘高产高效,提高养殖经济效益。     

调控反刍家畜瘤胃甲烷生成的措施

本文通过对反刍家畜瘤胃甲烷气体生成机理及控制其产量的措施的概述,以求提供有效的方法,在不显著影响反刍家畜的生产性能的情况下达到减轻瘤胃甲烷排放对大气环境造成污染的目的。     

PASSAT领驭2.0自动变速箱偶尔出现紧急运行状况检修分析

结合PASSAT领驭2.0轿车偶尔出现自动变速箱Ⅲ挡运行的紧急运行状况故障,分析了故障码、有关传感器电路,以及故障产生的原因,据此找出了故障点,并排除了故障。     

基于ADAMS的农用汽车前悬架仿真试验研究

为解决农用运输车前悬架存在振动过大等问题,基于ADAMS软件对农用汽车前悬架进行模拟仿真试验,以指导农用运输车前悬架的优化设计,使其具备更好的动力学性能。优化结果：上横臂长度、上横臂在汽车横向平面的倾角为332.6mm、12.5°,下横臂长度、下横臂在汽车横向平面的倾角为516.5mm、6.5°,车轮接地点的侧向滑移量由初始的19.783mm下降为3.2911mm。     

辽西北矿区矸石废弃地造林关键技术研究

矸石是在煤炭生产过程中产生的固体废弃物。我国是煤炭生产和消费的大国,全国每年矸石排放量约1．7亿t,已形成矸石山1500余座,压占大量土地,污染环境。文章在分析辽西北矿区矸石废弃地立地生态特征及研究该地区造林适用原则基础上,对造林关键技术进行研究分析,提出相应栽植技术要点,对该地区矸石废弃地植被恢复、改善生态环境具有实际指导意义。     

花生EMS诱变体系的探索及突变体鉴定

突变体创制对花生遗传改良和功能基因组学研究具有重要意义。为获得最佳诱变效果,本研究对不同基因型花生品种的EMS诱变条件进行摸索,发现花育22号、花育71、花育9301、狮头企和伏花生的最适诱变浓度分别为0.4%、0.5%、0.3%、0.5%和0.3%。通过对最适EMS浓度诱变处理下苗期芽长、株高、根长及根数目分析,发现5个品种虽然都能正常成苗,但均受到较严重的损伤。在预实验基础上,用浓度为0.4%的EMS溶液处理了大约5000粒花育22号种子,M1及M2世代性状调查显示突变群体表型丰富,出现株高、株型、荚果、籽仁、分枝数、叶形等表型变异株系,表型变异率为12.9%。本研究为花生遗传改良和功能基因组学研究提供了丰富的种质材料。     

不同肥料对春玉米干物质积累及转运的影响

以西辽河平原地区主推品种伟科702为供试品种,通过2015、2016两年田间试验,研究侧深施复合肥+硫包衣尿素(FS)、缓释肥+硫包衣尿素(HS)和常规施肥(CK)施肥方式下,春玉米干物质积累和转运变化规律。结果表明,缓释肥+硫包衣尿素处理,春玉米产量、器官干物质积累量及完熟期子粒干物质分配比例均高于复合肥+硫包衣尿素处理和常规施肥方式;转运率及子粒贡献率均低于复合肥+硫包衣尿素处理和常规施肥方式。缓释肥+硫包衣尿素施肥及复合肥+硫包衣尿素处理方式较常规施肥处理少追肥1次,综合考虑增产效果和成本因素,一次性侧深施缓释肥+硫包衣尿素,可作为研究地区玉米推荐施肥模式。