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941.
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944.
AIM: To investigate the expression of long non-coding RNA maternally expressed gene 3(MEG3) in colorectal cancer(CRC) cells, and to observe the effect of MEG3 on the invasion and migration of CRC cells. METHODS: The levels of MEG3 in human normal colon cell NCM460 and CRC cells SW48 and LoVo were detected by real- time PCR. MEG3 was over-expressed by plasmid transfection, and the effects of MEG3 on the invasion and migration of SW48 and LoVo cells were analyzed by Transwell assay and wound healing assay. The expression of matrix metalloproteinase(MMP) family proteins was determined by Western blotting. RESULTS: The level of MEG3 was down-regulated in CRC cells compared with normal colon cell NCM460. The invasion and migration of CRC cells were reduced after MEG3 over-expression. Transwell invasion and migration assays showed that the numbers of transmembrane SW48 and LoVo cells were smaller in MEG3 over-expression group than control group(CONCLUSION: The expression of MEG3 is down-regulated in CRC cells. Over-expression of MEG3 inhibits the invasion and migration of CRC cells. TIMP-2, MMP-2 and MMP-9 might play an important role in this regulation. 相似文献
945.
946.
在盆栽条件下对百里香和小花溲疏叶片的气孔特征、叶绿素含量及气体交换日变化进行了比较研究,结果表明:百里香的气孔密度明显高于小花溲疏,且气孔的长与宽比小花溲疏要小;百里香的叶绿素含量比小花溲疏高;百里香和小花溲疏都具有比较典型的双峰型光合速率日进程曲线,它们的气孔导度、蒸腾速率的日变化曲线与净光合速率的日变化基本一致;在一日内百里香净光合速率、气孔导度和蒸腾速率始终大于小花溲疏;百里香叶片气孔的结构特点、叶绿素含量的生理基础以及百里香植株的生物学特点是其净光合速率始终高于小花溲疏的主要原因,也使得百里香较小花溲疏更适应旱生环境. 相似文献
947.
在盆栽条件下对百里香和小花溲疏叶片的气孔特征、叶绿素含量及气体交换日变化进行了比较研究,结果表明:百里香的气孔密度明显高于小花溲疏,且气孔的长与宽比小花溲疏要小;百里香的叶绿素含量比小花溲疏高;百里香和小花溲疏都具有比较典型的双峰型光合速率日进程曲线,它们的气孔导度、蒸腾速率的日变化曲线与净光合速率的日变化基本一致;在一日内百里香净光合速率、气孔导度和蒸腾速率始终大于小花溲疏;百里香叶片气孔的结构特点、叶绿素含量的生理基础以及百里香植株的生物学特点是其净光合速率始终高于小花溲疏的主要原因,也使得百里香较小花溲疏更适应旱生环境。 相似文献
948.
不同粒型花生品种衰老过程中生理特性的变化 总被引:1,自引:0,他引:1
以不同粒型的花生品种为材料,研究衰老过程中叶片的生理特性及产量性状。结果表明:后期叶片NR、PAL的活性呈下降趋势,但大粒型品种较小粒型品种下降速度慢;开花50d以后,大粒型品种AsA-POD的变化呈抛物线下降(Y=-0.0492X^2+5.5791X-146.82);小粒型品种呈直线下降(Y=-0.553X+39.302)。MDA含量在开花后60—65d迅速上升,至成熟期,大粒型品种MDA含量较小粒型品种降低44.9%,百仁重、百果重平均分别增加46.8%、61.4%,产量提高33.2%。 相似文献
949.
ZHANG Rong-Yue WANG Xiao-Yan YANG Kun SHAN Hong-Li CANG Xiao-Yan LI Jie WANG Chang-Mi YIN Jiong LUO Zhi-Ming LI Wen-Feng HUANG Ying-Kun 《作物学报》1962,47(2):376-382
The aim of this study was to assess brown rust resistance of new sugarcane varieties bred in China and main cultivated varieties in sugarcane planting area, and screen the elite new brown rust resistant varieties for popularization and application in production. In total, 60 new varieties in the national regional test of new sugarcane varieties were tested in Kaiyuan and Lincang, and 34 main cultivated varieties were studied in Lincang, Puer, and Yuxi, Yunnan province, and Yizhou, Guangxi province, China, where the incidence of brown rust was particularly high. The resistance of these sugarcane varieties to brown rust was investigated under natural inoculation and molecular marker-assisted identification was used to detect the brown rust resistance gene Bru1. The results of field survey showed that 66 (70.21%) of the 94 new and main cultivated varieties were highly resistant to moderately resistant, and 28 (29.79%) were susceptible to highly susceptible. Molecular detection indicated that Bru1 gene was found among 54 (57.45%) of the 94 new and main cultivated varieties. Some main cultivated varieties that were currently planted across large areas such as Guitang 29, Guitang 44, Dezhe 03-83, Liucheng 03-1137, Yuetang 60, and Guitang 46 were highly susceptible to brown rust, and 31 new varieties such as Yuegan 48, Funong 09-2201, Guitang 08-120, Liucheng 09-15, Zhongzhe 1, Yunzhe 08-1609, Yunrui 10-187, and Zhongtang 1201 were resistant. Therefore, in the sugarcane planting areas with high incidence of brown rust and wet and rainy climates, more effort should be eliminated the main susceptible varieties and promoted the application of new resistant varieties. This will help to achieve a reasonable distribution of varieties, fundamentally control the outbreak of disease in sugarcane planting areas, and provide security for the high-quality development of sugarcane industry in China in the future. 相似文献
950.
浅析了贵州茶叶加工业与国内外比较的现状、存在问题、茶叶产业发展优势和加工生产潜力,提出了贵州茶叶加工业发展的对策建议和主要措施,通过确立发展目标和规划,建立优势区域原料生产基地和地方特色茶产业,以达到加快发展贵州茶叶加工业的目的。 相似文献