印度洋东部黄鳍金枪鱼的渔业生物学

根据在印度洋东部海域的延绳钓生产实践 ,对所渔获的黄鳍金枪鱼 (Thunnus albacares)群体的构成、繁殖等基本生物学特征进行了初步探讨。结果显示 ,渔获黄鳍金枪鱼纯重范围为 10～75 kg,叉长范围为 80～ 15 0 cm,纯重、叉长关系为 W=1.5 2 7× 10 -5L3,渔获构成月间变化明显 ,不同年龄个体性成熟与繁殖节律差异显著     

液相色谱-串联质谱法测定水产品中孔雀石绿的残留量

建立了液相色谱-串联质谱测定水产品中孔雀石绿及其代谢物残留的检测方法。样品经乙酸铵盐溶液和乙腈提取,经二氯甲烷萃取,PRS阳离子交换柱净化,然后选用电喷雾离子源,在正离子、多反应监测方式模式下进行定性,以内标法进行定量。方法的检出限为0.10μg·kg-1,工作曲线的线性范围为0.10~4.00μg·kg-1。在添加浓度为0.10~4.00μg·kg-1的范围内,孔雀石绿和隐色孔雀石绿的平均回收率均分别为83.4%和95.0%,相对标准偏差分别为6.23%和6.74%。     

翘嘴鳜反季节人工繁殖及苗种培育试验

对来源于鄱阳湖和高邮湖的两个野生翘嘴鳜群体,分别在水泥方池降低温度和减低光照培育以及在池塘网箱按常规培育。结果显示,水泥方池培育的雌鱼,成熟度正常、过熟和流产的比例,鄱阳湖群体为36.7%、40.0%和23.3%,高邮湖群体为40.0%、43.3%和16.7%,而雄鱼精子活力均正常;在池塘网箱培育的雌鱼均已过熟或流产,46.7%的雄鱼精巢完全退化。对成熟度正常和过熟的雌鱼各挑选6尾进行催产试验,正常组的催产率、单位体重产卵量、受精率、孵化率和出苗率均显著高于过熟组。试验共孵化出苗118.7万尾,育成1.3 cm的鱼苗96.3万尾和3~5 cm的鱼种68.1万尾。试验结果表明,采取降低温度和光照,能将翘嘴鳜在江苏地区的人工繁殖期推迟至7月底,从而实现高温期的批量育苗,为鳜鱼的反季节养殖奠定了基础。     

金枪鱼延绳钓船混合动力推进系统研究与分析

渔业节能减排和保护环境对促进国家海洋经济发展将起到重要作用。与传统的船舶动力系统相比,混合动力推进系统的电力推进具有调速范围广、驱动力大、易于正反转、体积小、布局灵活、安装方便、便于维修、振动和噪音小等优点。金枪鱼延绳钓船在作业时间内,主机处于低转速低负荷运行状态,燃油消耗率升高,出现积炭,导致增压器背压增加,可能引起喘振;主机受最低稳定转速限制,易造成航速忽快忽慢,不利于生产作业。根据金枪鱼延绳钓船作业方式和特点,提出分段驱动并联结构机电混合动力推进系统应用于金枪鱼延绳钓船,形成机电混合推进系统,降低了金枪鱼延绳钓船在航行及作业工况下对主机的依赖性,提高船舶推进系统冗余度,提高船舶放钓、收钓作业工况下的操纵灵活性,大幅度提高船舶操纵性和综合推进效率。通过经济适用性和环境保护性指标体系分析,与常规金枪鱼延绳钓船相比较,在放钓、巡钓和收钓作业等相同工况下,综合耗油量降低20%,系统可靠性,安全性和综合推进效率有一定提高。     

施肥对普陀樟苗木生长及养分吸收利用的影响

为了解不同施肥水平对普陀樟Cinnamomum japonicum var.chenii苗木生长及养分吸收利用情况的影响,设置了3个水平的田间试验,即不施肥(ck),常规施肥(T50,50 g·m-2)和增量施肥(T100,100 g·m-2),于2012年6月至9月观测苗高和地径,定期采集分析土壤和植物样品。结果表明:普陀樟苗木的苗高、地径、根部和茎部生物量均随施肥量的增加而增加(P<0.05);而叶生物量和总生物量在6月时随施肥量增加而升高(P<0.05),到9月时则呈先升后降的趋势(P<0.05)。苗木体内养分质量分数和单株养分含量随施肥量的增加而增加(P<0.05),不同处理均以叶片养分质量分数最高;养分吸收及利用率随着施肥的增加而降低(P<0.05),T50和T100处理各元素的吸收利用效率均为氮>磷>钾。到试验结束时,T50处理土壤养分质量分数与试验开始时持平,T100处理钾积累,ck引起钾亏缺。从移栽成活来看,T50苗木体内养分积累,有利于移栽成活,保持土壤养分平衡;而T100养分浪费。综合判断,普陀樟苗木最适需养量应为50 g·m-2到100 g·m-2,且较接近50 g·m-2。     

氮素形态对紫花苜蓿盛花期生长及结瘤固氮的影响

在完全营养液条件下,采用砂培法,研究了3种氮素形态配比NO3--N,NH4+-N和NO3--N∶NH4+-N为1∶1和0、105、210mg/L 3个氮素水平对紫花苜蓿盛花期生长及结瘤固氮的影响。结果表明:不同形态氮素处理下紫花苜蓿的株高、生物量、根瘤数、根瘤重、固氮酶活性和全氮含量均显著高于CK。同一氮素水平下,株高表现为NO3--N和NH4+-N混合培养下效果最好,NO3--N培养下次之,NH4+-N培养下最低;生物量、根瘤数、根瘤重、固氮酶活性和全氮含量NO3--N和NH4+-N混合培养下效果最好,NH4+-N培养下次之,NO3--N培养下最低;茎叶比则是NO3--N培养下最大,NH4+-N培养下次之,NO3--N和NH4+-N混合培养下的最低,各氮素形态处理间差异显著(P0.05)。随着氮素水平的增加,各形态配比下紫花苜蓿的株高、生物量、根瘤数、根瘤重、固氮酶活性和全氮含量呈现增加的变化趋势,茎叶比呈现减小的变化趋势。NO3--N+NH4+-N的浓度为210mg/L时,紫花苜蓿的株高、生物量、根瘤数、固氮酶活性和全氮含量取得最大值,茎叶比取得最小值,根瘤重则在NO3--N+NH4+-N的浓度为105mg/L时取得最大值,紫花苜蓿在混合态氮培养下生长最好。     

Role of ACSL3 expression in suppressing prostate cancer cell metastasis

AIM:To analyze the difference of long-chain acyl-CoA synthetase 3 (ACSL3) expression between normal prostate epithelial cells and prostate cancer cells.METHODS:ACLS3 mRNA expression between normal prostate epithelial cells and prostate cancer cells was compared using RT-PCR. Meanwhile, ACSL3 gene was amplified from prostate cancer cells, and the eukaryotic expression plasmid pCDNA3.1(+)-Flag-ACLS3 and lentivirus Lenti-ACLS3 were constructed. After transfection of ACSL3-plasmid and lentivirus into the prostate cancer cells, ACSL3 expressive was detected by RT-PCR and Western blotting, and then Matrigel invasion assay was performed to investigate the alteration of the invasive ability of the prostate cancer cells with over-expression of ACSL3. RESULTS:A significant difference of ACSL3 mRNA level between normal prostate epithelial cells and prostate cancer cells was observed. ACSL3 was highly expressed in localized prostate cancer cells compared to metastatic prostate cancer cells, while ACSL3 expression was higher in androgen-dependent prostate cancer cells than that in androgen-independent prostate cancer cells. Furthermore, the eukaryotic expression plasmid and lentivirus containing ACLS3 gene were successfully constructed. The prostate cancer cell line which stably over-expressed ACLS3 was established. Up-regulation of ACSL3 inhibited the invasive ability of prostate cancer cells. CONCLUSION:ACSL3 plays an antagonistic role in invasiveness of prostate cancer.     

《动物寄生虫》课程考核方式的改革探讨

为了全方面地进行课程改革,使课程改革取得更好的效果,金华职业技术学院主要从理论考核、实践考核以及结合适当的论文写作等三方面入手,针对《动物寄生虫》课程考核方式的改革进行了探讨,以期更好地服务于课程全面改革的实践。     

投喂频率对凡纳滨对虾生长和水质的影响

研究了不同投喂频率(1,2,3,4和5次·d-1)对凡纳滨对虾生长、存活率和水质的影响。试验在室内2m3的圆形水泥池(d=1.6m)中进行,采用全封闭养殖模式,凡纳滨对虾(初始重量为0.24g)养殖密度为50尾·m-3,投喂人工配合饲料(粗蛋白含量为42.98%)。通过7周的试验,结果表明,投喂频率为1～4次·d-1时,随着投喂频率的增加,对虾增重率显著增加;投喂频率由4次·d-1增加到5次·d-1时,对虾增重率略有增加,但差异不显著。凡纳滨对虾的成活率在1次·d-1时最低,2次·d-1时最高,2～5次·d-1时随着投喂频率的增加而降低(投喂频率为1和2次·d-1之间、3和4次·d-1之间差异显著)。投喂频率为3次·d-1时饲料系数最低,蛋白质效率最高。随着投喂频率1～3次·d-1增加,饲料系数逐渐显著降低,蛋白质效率显著递增;而投喂频率从3～5次·d-1增加时,饲料系数增大,蛋白质效率逐渐下降(3和4次·d-1差异显著;4和5次·d-1之间差异不显著)。投喂频率对水环境中的pH值、H2S含量、COD、盐度等水质指标没有显著影响;在养殖后期水环境中投喂频率3～5次·d-1的NH 4和NO-2浓度较投喂频率1～2次·d-1时高。     

Effect of dietary iron supplement on growth, haematology and microelements of juvenile grouper, Epinephelus coioides

An experiment was conducted to investigate the effect of dietary iron supplement on growth, haematology and microelements of juvenile grouper, Epinephelus coioides. Casein–gelatine‐based diets supplemented with 0, 50, 100, 150, 200 and 250 mg kg⁻¹ iron from ferrous sulphate were fed to grouper (mean initial weight: 21.0 ± 0.2 g) for 8 weeks. Weight gain was highest in fish fed the diet supplemented with 100 mg kg⁻¹ iron, intermediate in fish fed diets with 50, 150, 200 and 250 mg kg⁻¹ iron and lowest in fish fed the basal diet. Feed efficiency followed a similar trend except that the lowest value was in fish fed the basal diet and the diet supplemented with 250 mg kg⁻¹ iron. Hepatic iron was highest in fish fed diets supplemented with iron ≥100 mg kg⁻¹, followed by fish fed diet with 50 mg kg⁻¹ iron and lowest in fish fed the basal diet. The whole‐body iron was lowest in fish fed the basal diet but not significantly different from other groups, as judged by anova . Iron supplement to the basal diet had no significant effect on haematological parameters (red blood cell count, haematocrit and haemoglobin), hepatic copper concentration or manganese, zinc concentration in liver and whole body. Broken‐line analysis of hepatic iron indicated that iron supplementation of 100 mg kg⁻¹ satisfied the hepatic iron storage and that further supplementation did not expand the iron status.